A perfusion module and a perfusion culture system are provided, including: a bioreaction module, including at least one bioreactor; a perfusion module, including at least one perfusion chamber, each perfusion chamber is connected to a pressure regulating pipe for inflow or outflow of an airflow; when there are multiple perfusion chambers, every two adjacent perfusion chambers are connected to each other; at least one bioreactor, the perfusion module, the filtration module are communicated in sequence, if there are more than two perfusion chambers, all of perfusion chambers are set side by side; a cell culture fluid in at least one bioreactor flows into at least one perfusion chamber, flows out from at least one perfusion chamber into at least one filter, and a permeate from at least one filter flows into a harvesting device. The present disclosure avoids high shear forces in the perfusion culture system.

The invention relates to a fermenter vessel (1) of a biogas plant, having at least one height-adjustable agitator (8), wherein the agitator (8), in the fermenter vessel (1), is guided with a guide element (17) on a guide rail (9) oriented approximately vertically in a vertical axis direction and is held in height-adjustable fashion by means of a traction cable (10). According to the invention, a traction force measuring device (22; 25) is provided for directly and/or indirectly measuring the traction force on the traction cable (10) in accordance with a cable tension, such that, by means of the traction force measuring device (22; 25), in the case of a preferably controlled height adjustment of the agitator (8), varying traction force measurement signals are generated in accordance with varying traction forces and are supplied to an evaluation device (20).

A docking device for receiving a portable device. The docking device includes a base including a substantially planar upper surface for supporting a portable device. The docking device includes an array of a plurality of engagement members. Each engagement member in the plurality of engagement members includes a fixed body protruding upwardly from the substantially planar upper surface of the base. Each engagement member includes a spring including a fixed end portion and a free end portion for engagement with the portable device. The fixed end portion interfaces with the fixed body. The free end portion protrudes from the fixed end portion obliquely towards the substantially planar upper surface of the base. The free end being movable along a deformable gap between a first surface of the fixed body and a second surface of the spring.

The present invention relates to a laboratory device, wherein the laboratory device has an outer housing which defines an interior of the device, wherein the laboratory device is designed to assume an operating state at which a pressure in the interior of the device is lower than an ambient pressure in the environment of the laboratory device. The present invention also relates to the use of the laboratory device in a clean room.

The bioreactor can use a number of different culture growing structures for culturing cells including a lattice structure and a support matrix structure. The culture growing structure whether it be a support matrix using wicking and/or a lattice structure may be coated with a thermal responsive polymer. The material of interest growing on the culture growing structure can be removed by changing the temperature that the thermal responsive polymer on the surface of the culture growing structure is exposed to and thus in some cases release the thermal responsive polymer along with the material of interest from the remainder of the culture growing structure.

Example monitoring devices, systems and methods for use are provided. The devices can comprise one or more paths. In an embodiment, a first fluid path is connected to a sensor manifold, the sensor manifold comprising one or more sensors for sensing one or more characteristics of a fluid, and a second fluid path connected to a second sensor component, the second sensor component comprising a density sensor for sensing the density of the fluid.

The present invention is directed towards a microfluidic device comprising a first compartment comprising an inlet that is connectable to a fluidic control unit and a second compartment, wherein the first and second compartments are connected by a micrometer channel so as to allow fluid communication between the two compartments. The device also comprises an air-lock element in fluid communication with the second compartment and the air-lock element is configured so that in use the internal atmosphere of the device is sealed from the external atmosphere and so that when fluid is introduced or withdrawn from the first compartment via the inlet the air-lock element maintains an overall constant pressure within the device.

The present invention is also directed towards a method of manufacturing the microfluidic device, a kit-of-parts comprising the microfluidic device and a method of using the microfluidic device for accommodating, growing, culturing, isolating, treating and/or processing cells.

The present disclosure provides a cell separation apparatus for a bioreactor, which comprises: a cell separation component, which is arranged in a tank and immersed below the liquid level of the mixture and includes a filter membrane and a liquid guiding groove; and a tangential flow driving component, which includes one or more blades. The cell separation apparatus implements a low shear force and the filter membrane is not blocked easily. The present disclosure also provides a stirring system including a central shaft and a multi-layer paddle component. The multi-layer paddle component includes an upper paddle component, an intermediate paddle component and a bottom paddle component arranged around the central shaft.

A system for incubating one or more cells and/or organotypic cultures for biological investigation, in particular for toxicology assessment, comprising a bio-incubator and a pressure system fluidly connected with the bio-incubator. The pressure system is a cyclic gas pressure system configured for cyclically varying the gas pressure in the bio-incubator between a negative pressure and a positive pressure compared to the atmospheric pressure, so as to reproduce the pressure conditions in lungs of a living mammal. The system is remarkable in that the cyclic gas pressure system comprises a feedthrough with a pipe configured to deviate the air influx from the one or more cells and/or organotypic cultures.

Embodiments described herein relate generally to devices, apparatuses, and systems with embedded electrodes for rowing, maintaining, and/or using 3D tissues in vitro. The devices, apparatuses, and systems described herein can provide scalable, automated tissue stimulation.