An automated method of T cell scale down processing. The method including: activating T cells by automatically contacting the T cells with one or more activation reagents; transducing the T cells by automatically contacting the T cells with a recombinant viral vector; automatically inoculating T cells; automatically expanding the T cells; optionally, automatically debeading the T cells; and automatically harvesting the T cells. A system for an automated method of T cell scale down processing.

The present disclosure relates to compositions and methods for combinatorial assessment of nanoscale droplets, as specifically exemplified by massively parallel assessment of spatially-directed (while agnostic as to precise droplet content) combinations of droplets harboring distinct and independently identifiable microbial types and/or chemical compounds or mixtures. More particularly, the disclosure relates to a platform and methodologies for identifying advantageous (including synergistic, additive, etc.) microbial interactions and/or chemical compound or mixture interactions with microbes in a manner that allows for binary, trinary, etc. combinatorial assessments to be performed across a range of many discrete input types of microbes (e.g., 6-16 or more discrete input microbial types), to an extent capable of approaching comprehensive sampling and measurement of microbial community combinations from a selected panel of microbial inputs, optionally also in the presence of chemical compounds or mixtures (e.g., test compounds or mixtures for antimicrobial effect).

Provided is a non-linear model predictive control (NMPC) system for automatic and optimum start-up of an anaerobic digestion (AD) system. The NMPC provides an optimum set of values of manipulated variables for controlling some of the key AD process variables during start-up. The NMPC based automatic start-up system was evaluated against a virtual AD process plant scenario involving a high rate AD reactor treating a readily biodegradable carbohydrate based substrate.

A cell culture system includes: a plurality of cell culture channels each of which includes a fluidic device in which cells are cultured, a pump which makes a liquid flow into the fluidic device, a detection unit which detects a state of the cells that are cultured, and a controller which controls the pump and the detection unit; a measurement unit that measures culture environment of the cells which are cultured in the plurality of cell culture channels; and an information processing apparatus that adjusts the culture environment such that an absolute value of a difference between evaluation values for the states of the cells that are cultured in the plurality of cell culture channels is a threshold value or less, on the basis of the state of the cells detected by the detection unit and the culture environment of the cells measured by the measurement unit.

There is provided a cell evaluation device comprising an acquisition unit that acquires an image set of a plurality of cell images in which a plurality of cells having a protrusive structure are imaged in time series during culture; a reception unit that receives designation of a cell of interest which is a cell of interest to a user, the cell being at least one cell among the plurality of cells which are shown in one cell image of interest other than an oldest cell image in the image set; a specifying unit that captures a growth process of the protrusive structure based on the image set to specify a protrusive structure of interest, which is the protrusive structure extending from the cell of interest in the cell image of interest; and a display control unit that performs control to display the protrusive structure of interest in a display form different from that of other protrusive structures in the cell image of interest.

An apparatus for measuring an electrophysiological signal of a three-dimensional (3D) cell and a method of fabricating the apparatus. The apparatus includes a flexible substrate including a plurality of protrusions, and a conductor formed on the plurality of protrusions, and regions other than the plurality of protrusions are partially removed from the flexible substrate.

A biocontainment vessel includes a vessel structure including a structural composition and an enhancement composition associated with the structural composition. The enhancement composition includes a co-polymer. The co-polymer is a poly(glycerol sebacate) or a poly(glycerol sebacate urethane). The enhancement composition may also include an augmentation agent associated with the co-polymer. The enhancement composition is located with respect to the structural composition such that the enhancement composition benefits biological cells contained in the biocontainment vessel. A composition includes a co-polymer and an augmentation agent contained by the co-polymer. A method of containing biological cells includes placing the biological cells in an augmented biocontainment vessel and storing them in the augmented biocontainment vessel under predetermined conditions. An augmented substrate includes a substrate and an enhancement composition coating a surface of the substrate.

A system and method for growing and maintaining biological material including producing a protein associated with the tissue, selecting cells associated with the tissue, expanding the cells, creating at least one tissue bio-ink including the expanded cells, printing the at least one tissue bio-ink in at least one tissue growth medium mixture, growing the tissue from the printed at least one tissue bio-ink, and maintaining viability of the tissue.

The present invention aim at providing a novel animal cell growth promoter. Specifically, a culture supernatant of an embryonic membrane derived from an avian or reptilian fertilized egg is use as an animal cell growth promoter. For example, by adding, to an animal cell culture medium, an animal cell growth promoter comprising, as an active ingredient, a culture supernatant of an embryonic membrane derived from an avian or reptilian fertilized egg, the animal cell growth can be promoted.

A high-throughput, three-dimensional microelectrode array for in vitro electrophysiological applications includes a 3D printed well plate having a top face and bottom face, and a plurality of culture wells formed on the top face of the well plate. Each culture well includes a plurality of vertical microchannels on the top face and microtroughs formed on the bottom face and communicating with the microchannels. A conductive paste fills the microtroughs and the microchannels and forms self-isolated microelectrodes in each culture well and conductive traces that communicate with the self-isolated microelectrodes.