A system for an automated production of high cell density seed culture, HCDC, preparations, for cryo-preservation comprising a cell expansion device for growing a HCDC in a medium inside a device volume of the cell expansion device, at least one sensor for collecting data by at least temporally monitoring at least one parameter being indicative of the physiological state of the HCDC; and a control unit for processing the collected data to determine whether at least one predetermined criterion is fulfilled by the monitored parameter to control, based on whether the at least one predetermined criterion is fulfilled by the monitored parameter, at least a partial exchange of the medium by a cryo-protective agent, CPA.

A method for analysis of yeast includes: receiving a microscopic image of yeast by a cloud server (2901), the microscopic image including a scaling pattern for determining a magnification; determining the magnification by the cloud server based on the scaling pattern (2902); and analyzing, by the cloud server, the microscopic image based on the magnification to obtain an analysis result (2903).

Systems (100) and methods for perfusion control in a bioreactor (120) are provided. The system (100) comprises a media container (110) connected to the bioreactor (120) and weighing scales (113, 123) measure weight of the bioreactor (120) and the media container (110). A plurality of controllers (225, 245) are connected to the weighing scales and configured to continuously feed the media from media container (110) to the bioreactor (120) at a user defined rate using a motor pump (112). A filter (130) is provided to receive feed from the bioreactor (120) through a recirculation line (121) and a permeate flow line (141) is connected to the filter (130) to flow out the permeate from the filter (130). When weight of the bioreactor (120) goes beyond the upper (U) or lower (L) permissible weight limit, a controller (225) connected to the weighing scale of the bioreactor (120) sends a signal to operate the permeate motor pump (142) either to flow out the permeate from the filter (130) or to stop the motor pump (142).

A device for simulating a function of a tissue includes a first structure, a second structure, and a membrane. The first structure defines a first chamber. The first chamber includes a matrix disposed therein and an opened region. The second structure defines a second chamber. The membrane is located at an interface region between the first chamber and the second chamber. The membrane includes a first side facing toward the first chamber and a second side facing toward the second chamber. The membrane separates the first chamber from the second chamber.

The present disclosure provides an ultrasound therapy system and a dose control method. The system includes: a control device; a first ultrasound irradiation device configured to generate multiple groups of ultrasound irradiation doses driven by the control device and conduct ultrasound irradiation on a cell culture device with multiple groups of abnormally proliferating living cells; a characterization image capture device configured to capture performance characteristic data of living cells in the cell culture device, where the control device is further configured to determine an ultrasound irradiation dose corresponding to at least one group of abnormally proliferating living cells with a cell target characteristic characterization as a target ultrasound irradiation dose according to the performance characteristic data; and a second ultrasound irradiation device configured to conduct ultrasound irradiation of the target ultrasound irradiation dose on a living organism with abnormal proliferation.

Disclosed herein is a device comprising a microelectrode comprising cells cultured on a surface of the microelectrode and a porous membrane comprising an upper surface comprising cultured cells. Further, devices and methods for in in-vitro models of the blood-brain barrier (BBB) and for modeling the transport across this barrier are disclosed.

A method and system for studying cell viability and protein aggregation. In one aspect, the method relies on speckle information to analyze cells or other structures present in a fluid. A probe for measuring in situ structures includes a tip section having a sample detection region and a camera provided with image sensors.

The purpose of the present invention is to efficiently dispense cells while reducing the risk of incorporating bacteria, etc. A cell dispensing device 1 includes: a first tube 23a which connects a container 21 and a dispensing container 22; a syringe pump 24 attached to a second tube 23b connected to a branching portion 31 formed on the first tube 23a; and a buffer tank 25 provided between the syringe pump 24 and the branching portion 31. In addition, a cell suspension contained in the container 21 is temporarily stored in the buffer tank 25 by means of the syringe pump 24, and the cell suspension stored in the buffer tank 25 is delivered to the dispensing container 22.

A controlling system is provided for operating an examination system configured for microscopic examination of a sample. The examination system includes a microscope, an incubation environment conditioning unit connected to the microscope, and a user interface. The examination system provides an incubation mode in which a sample chamber is incubated by supplying an incubation atmosphere generated by the incubation environment conditioning unit. The controlling system is configured to receive a target setpoint of at least one examination parameter upon user input via the user interface, select, based on the received at least one target setpoint, predefined adjustment setpoints for at least one incubation environment parameter of the incubation mode and for at least one microscope parameter, and operate the incubation environment conditioning unit and the microscope based on the selected adjustment setpoints.

An apparatus, that relates to the field of in vitro fertilization, is provided for the combined incubation and vitrification of a biological material. The apparatus can be configured to allow for automatic incubation and vitrification of a viable biological material. Thereby predetermined protocols for handling the biological material can be performed precisely and accurately thus avoiding errors and deviations from the intended protocol, as caused by manual human intervention.