Aspects of the present disclosure relate to a method including receiving, from a user interface on a display device of a computing apparatus, a dilution profile that includes a plurality of profile fields relating to a series of test instances for a plurality of culture devices. The method includes storing the dilution profile in a memory of a computing apparatus. The method includes receiving a request to form a worklist for reading the culture device. The worklist comprises a plurality of worklist fields that correspond to at least some of the plurality of profile fields in the dilution profile. The method includes determining that the dilution profile applies to the worklist and populating, responsive to the determination that the dilution profile applies and by the computing apparatus, at least some of the plurality of worklist fields with the plurality of profile fields in the dilution profile from the memory.

A microphysiological platform described herein includes a fluidic synthesizer with a first fluid input selectively coupleable to a source of a first input fluid solution and a second fluid input selectively coupleable to a source of a second input fluid solution. The fluidic synthesizer further includes a fluid output. The microphysiological platform further includes a fluid addressing system with a fluid input fluidically coupled to the fluidic synthesizer fluid output. The fluid addressing system further includes a first fluid output and a second fluid output. The microphysiological platform further includes a first microphysiological device with a fluid input fluidically coupled to the first fluid output of the fluid addressing system and a second microphysiological device with a fluid input fluidically coupled to the second fluid output of the fluid addressing system.

Spectral data indicating an intensity of electromagnetic waves, which have been emitted to a cell suspension including a cell and a culture solution and have been subjected to an action of the cell suspension, for each wave number or wavelength is acquired. Preprocessing is performed on the spectral data. A soft sensor, which receives processed data obtained by the preprocessing as an input and outputs state data indicating a state of the cell or the culture solution, is constructed by machine learning using a plurality of combinations of the processed data and the state data as training data. The processed data for the spectral data acquired for a cell suspension including a cell which is being cultured is input to the soft sensor, and the state data output from the soft sensor is acquired.

Systems and methods to provide low carbon intensity (CI) transportation fuels through one or more targeted reductions of carbon emissions based upon an analysis of carbon emissions associated with a combination of various options for feedstock procurement, feedstock refining, processing, or transformation, and fuel product distribution pathways to end users. Such options are selected to maintain the total CI (carbon emissions per unit energy) of the transportation fuel below a pre-selected threshold that defines an upper limit of CI for the transportation fuel.

Systems and methods are provided for evaluating a tissue that utilize a resistance to represent pressure of a fluid or gas passing through the tissue and a capacitance to represent compliance of the tissue.

Systems and methods for rapid determination of microorganism growth and antimicrobial agent susceptibility and/or resistance are disclosed.

In one aspect, a method for automated analysis of samples from a bioreactor is provided herein, the method including: drawing at least one sample from a bioreactor; pressurizing the drawn at least one sample into a sample flow; purifying at least one target protein in the sample flow using a first liquid chromatography apparatus to create a purified sample flow; splitting the purified sample flow into a purified sample fraction flow and an effluent flow; and, analyzing the at least one target protein in the purified sample fraction flow using a second liquid chromatography apparatus. Advantageously, the subject invention provides for an automated two-step liquid chromatography process utilizing first dimension liquid chromatography for purification and second dimension liquid chromatography for analysis.

Devices for treatment of cells are disclosed. The devices include an elongated housing and at least one hollow fiber semi-permeable membrane positioned within the housing having a plurality of pores dimensioned to prevent passage of the cells to be treated. Systems for treatment of cells including the device are disclosed. Methods of treating cells, including transducing cells and activating cells, are also disclosed. The methods include introducing a biosample with cells to be treated into the device, introducing media to suspend and release treated cells into the device, and discharging the treated cells from the device.

The invention relates to a system (100) for monitoring deviations of a state of a cell culture in a bioreactor (104, 106) from a reference state of a cell culture in a reference bioreactor (102). The bioreactor comprises the same medium (M1) as the reference bioreactor. The system comprises: •—a storage medium (114) comprising: •a PACO-reference profile (116) indicative of a deviation of a CO2 off gas rate (ACO.sub.R-M-.sub.ti) measured in the reference bioreactor from a predicted CO2 off gas rate (ACO.sub.R-EXP-ti) of the reference bioreactor; •a data object comprising a medium-specific relation (136) between the pH value of the medium (M1) and a respective fraction of CO2 gas in a gas volume when said medium is in pH-CO2 equilibrium state with said gas volume and lacks the cell culture; •—an interface (128) for receiving (212) a current CO2 off gas rate (ACO.sub.Bi-M-ti, ACO.sub.B2-M-.sub.t i) and a current pH value (pH.sub.Bi-ti) of the medium of the bioreactor (104, 106); •—a comparison unit (130) configured for computing (214, 216): •a PACO value (PACO.sub.B1-tir PACO.sub.Bi-ti) the PACO-value being indicative of a deviation of a CO2 off gas rate (ACO.sub.Bi-M-ti, ACO.sub.B2-M-.sub.ti) measured in the bioreactor from a predicted CO2 off gas rate (ACO.sub.B1-EXP-ti, ACO.sub.B2-E xp-.sub.t i). a difference between the computed PACO value (PACO.sub.Bi-ti, PACO.sub.B2-ti) and a respective reference PACO value (PACO.sub.R-ti) in the PACO-reference profile (116).

A liquid filtration system comprising a syringe pump comprising a gas chamber and a movable plunger, wherein the gas chamber has an aperture at a first end and the plunger forms a seal within the internal walls of the chamber; a liquid chamber having two openings, the openings positioned at opposite ends of the chamber and the first opening connected to the aperture of the gas chamber; a bioreactor in fluidic communication with the second opening of the liquid chamber; a filter arranged to filter liquid passing between the bioreactor and the liquid chamber, the filter comprising a permeate outlet for removing filtered liquid; wherein, in use, the plunger may be moved in a reciprocating motion causing a corresponding movement of gas which drives liquid alternately between the liquid chamber and the bioreactor such that liquid passes through the filter and filtered liquid may be removed via the permeate outlet.