The present invention provides a method for separating a stromal vascular fraction (SVF) from adipose tissue by using a non-contact ultrasonic device comprising a power supply unit, an ultrasonic oscillation unit, and an ultrasonic vibration unit; and a non-contact ultrasonic device used therefor. Specifically, the present invention provides a method for separating a stromal vascular fraction (SVF) from adipose tissue, and a non-contact ultrasonic device used therefor, in which the stromal vascular fraction can be extracted from adipose tissue with high efficiency while minimizing cell contamination and destruction by using a non-enzymatic method and a non-contact ultrasonic device.

The invention generally relates to cells and compositions comprising same for use in cell therapy, to methods of obtaining same, and to use of same in cell therapy. In one aspect, the invention provides a method for forming a cell composition from a tissue sample, the method comprising: providing a tissue sample comprising cells; contacting the sample with a polymer in binding conditions, said binding conditions being conditions that enable binding of cells in the sample to the polymer, so that said cells are bound to the polymer; culturing the cells bound to the polymer under conditions and for a time that allows the cell number to increase; providing conditions to induce a phase change of the polymer; thereby forming a cell composition from a tissue sample.

Provided herein is technology relating to processing biological samples and particularly, but not exclusively, to systems and apparatuses for dissociating biological tissues into viable cells.

The present invention concerns a solids discharge module (1) for discharging solids out of devices (100) carrying mixed fluid, in particular comminuting devices for solids contents in a mixed fluid, comprising a solids inlet (3), coupling means (31) for connecting the solids inlet (3) to a device (100) carrying a mixed fluid, a solids outlet (5), a closing member (9) which is arranged at the solids outlet (5) and is reciprocable between a release position and a blocking position, and a conveyor chamber (7) extending from the solids inlet (3) to the solids outlet (5). According to the invention it is proposed that a filling state detector (13) is provided which is adapted to detect and display a solids accumulation in the conveyor chamber (7).

Disclosed are methods and devices for processing lipoaspirate that include mechanically-processing harvested lipoaspirate in a liposuction filter canister. In some embodiments, the devices are liposuction devices that include a lipoaspirate processing unit for mechanically-processing lipoaspirate. The mechanical processing reduces the average size of adipose tissue pieces in the lipoaspirate without substantially rupturing lipocytes therein.

The present disclosure generally relates to a method for controlled pretreatment of lignocellulosic biomass. The method comprises the steps of: Pretreating (S10) a lignocellulosic biomass material in a pretreatment arrangement, the pretreating comprising impregnating (S10A) the lignocellulosic biomass with an SO2 feed in an impregnation vessel of the pretreatment arrangement; collecting (S20) a number of process parameters of the pretreatment, which process parameters include at least a feed parameter related to the total amount of lignocellulosic biomass input to the pretreatment arrangement, and a dry matter parameter related to the dry matter content of lignocellulosic biomass input to the pretreatment arrangement; and adjusting (S30) the SO2 feed in response to the process parameters.

A system that easily and stably separates various living cells from a living body-derived tissue, the system including: a mincing unit that minces the living body-derived tissue based on a parameter; a measurement unit that acquires information regarding the living body-derived tissue being minced; and an analysis unit that calculates a ratio of impurities to the living body-derived tissue being minced from the information acquired by the measurement unit. Methods for separating various living cells from a living body-derived tissue are also disclosed.

A deagglomerator for use in resizing masses of cells. The deagglomerator may include a plurality of apertures defined by a plurality of front and back edges. The masses of cells may be passed through the plurality of apertures from the front to the back, and from the back to the front, repeatedly. The deagglomerator may also include a plurality of blades that may aid in the deagglomeration of the cell masses. The deagglomerator may be configured between two syringes so that the tissue may be passed back and forth from the first syringe through the device to the second syringe, and then back again from the second syringe through the device and to the first syringe. In this way, the masses of cells may be properly deagglomerated.

The present invention concerns a single use aseptic kit comprising: a disaggregation module for receipt and processing of material comprising solid mammalian tissue; and a stabilisation module for storing disaggregated product material, wherein each of said modules comprises one or more flexible containers connected by one or more conduits adapted to enable flow of the tissue material there between; and wherein each of said modules comprises one or more ports to permit aseptic input of media and/or reagents into the one or more flexible containers. The invention further relates to an automated device for semi-automated aseptic disaggregation and/or enrichment and/or stabilisation of cells or cell aggregates from mammalian solid tissue comprising a programmable processor and the single use aseptic kit. The invention further relates to a semi-automatic aseptic tissue processing method.

A method for processing biological material containing stringy tissue in a container having a tissue collector disposed in a tissue retention volume on one side of an internal filter includes washing biological material contained in the tissue retention volume with wash liquid to the tissue retention volume and allowing the wash liquid and rotating the tissue collector disposed in the tissue retention volume relative to the container in a first direction of rotation about an axis of rotation to sweep the teeth positioned on the tissue collector through the biological material and to collect stringy material on the tissue collector.