Methods of treating animal organic material are disclosed herein. The methods include diluting the animal organic material to produce an organic material slurry, anaerobically digesting the organic material slurry to produce a biogas and a digestate, separating the digestate to produce a digestate solids and a filtrate, removing ammonia from the filtrate, removing organic contaminants and divalent anions from the filtrate, concentrating the filtrate to produce a retentate and a permeate, combining the digestate solids and the retentate to produce a solids product, and returning the permeate to dilute the animal organic material. Systems for treatment of animal organic material are also disclosed herein. The systems include a dilution tank, an anaerobic digester, a first solids-liquid separation subsystem, an ammonia-reducing column, a second solids-liquid separation subsystem, a production water storage tank, and a solids product tank.

Disclosed herein are methods, systems, and compositions for the uniform pretreatment of biomass within seconds with low inhibitor formation. The pretreatment process is used to convert biomass to a fuel, sugars, or other useful chemicals by subjecting the feedstock to a rapid retention time under pressure and temperature and/or chemical reactant. The system includes at least one high pressure, steam impermiable plug and a continuously-operating valve discharge apparatus to discharge pretreated feedstock while maintaining uniform pressure on the pretreatment system.

Moisturized organic material is treated by communicating with a vacuum pump (44) to selectively reduce pressure within a vessel (12) below atmospheric pressure and to a vaporization point, such as at 30 to 17.8° C., for a period of time sufficient to create an internal pressure to rupture cell walls. While being subjected to vacuum below atmospheric pressure, the vessel (12) is rotated to homogenize the moisturized organic material. The organic material can be moisturized by non-potable water including moisture collected by a moisture collector (44b) in the conduit (44a) between the vessel (12) and the vacuum pump (44) and by chilled feed water acting as the seal fluid in the vacuum pump and heated while flowing through a heat exchanger (148) of the moisture collector (44b). Heat can be added by a heater (46) to raise the temperature of low ambient temperature moisturized organic material.

A method and apparatus of aseptic processing and production of cells in a non-sterile enclosure apparatus without chemical biocides is disclosed, by controlling the level of humidity throughout the enclosure to 25% relative humidity (RH) or less, and preferably 20% or 15% or less RH. In addition, the temperature is controlled to 37° C., and consistent gas flow is maintained the enclosure. Colony forming units from microbial contamination detected by environmental monitoring within the enclosure are significantly reduced in this method.

fermelnters or other processes for the treatment or conversion of organic substances, and also for improving viscosity, in which specifically required, technological or biological procedures are combined in one mixing and combi-hydrolysis tank. The device has one or more descending walls for separating the gas chamber in the top part of the tank, one descending wall of which separates the gas chamber of the tank opening from the other tank, and optionally a further descending wall, the lower end of which is permanently located above the lowest fluid level and which separates the gas chamber of the pump chamber from the other tank. The device optionally has an ultrasonic module for treating a recirculated material from an advanced fermentation stage or from the device according to claim 1, and also a gas supply system in the lower zones of the feed and/or pump chamber for hydrogenous gases, produced in the process or supplied externally, for stimulating methane formation by means of hydrogen-oxidising archaea.

A method and apparatus of aseptic processing and production of cells in a non-sterile enclosure apparatus without chemical biocides is disclosed, by controlling the level of humidity throughout the enclosure to 25% relative humidity (RH) or less, and preferably 20% or 15% or less RH. In addition, the temperature is controlled to 37° C., and consistent gas flow is maintained the enclosure. Colony forming units from microbial contamination detected by environmental monitoring within the enclosure are significantly reduced in this method.

A process for producing ethanol from lignocellulosic biomass includes adding at least one of sulfur dioxide and sulfurous acid to the lignocellulosic biomass to provide an equivalent sulfur dioxide loading of at least 10 wt % sulfur dioxide to dry lignocellulosic biomass. The acidified lignocellulosic biomass is pretreated at a temperature above about 185° C. and for a pretreatment time less than about 10 minutes, to provide a pretreated biomass composition wherein the biomass is readily hydrolyzed by enzymes. Advantageously, sulfur dioxide from at least one of the flash stream and a stream derived from the flash is recovered and recycled back into the process.

An organic waste digester system is provided. The system includes a heated hopper unit within the housing to receive organic waste. An agitation mechanism mixes the organic waste along with a microbe mixture to aid breakdown of the waste. Liquefied organic waste is discharged through an outlet and conveyed to a drying unit downstream of the hopper unit. The drying unit includes a microwave dryer unit.

The disclosure relates to an incubation device comprising a first incubation chamber, wherein a sample carrier holder and an examination device for examining a sample that is arranged in the sample carrier holder are arranged in the first incubation chamber, wherein the first incubation chamber has a connection opening for connecting to a second incubation chamber and an inlet opening for introducing the sample into the first incubation chamber, wherein a connection device is arranged at the connection opening, and wherein the connection device can be connected to a connection device which is arranged at a connection opening of a second incubation chamber. The disclosure additionally relates to a modular incubation system comprising the incubation device.

A dry grind biochemical production process and system with front end milling method is provided for improving biochemical and/or by-product yields, such as oil and/or protein yields. In one example, the process includes grinding corn kernels into particles then mixing the corn particles with a liquid to produce a slurry including oil, protein, starch, fiber, germ, and grit. Thereafter, the slurry is subjected to a front end milling method, which includes separating the slurry into a solids portion, including fiber, grit, and germ, and a liquid portion, including oil, protein, and starch, then milling the separated solids portion to reduce the size of the germ and grit and release bound starch, oil, and protein from the solids portion. The starch is converted to sugar, and a biochemical is produced therefrom then recovered. Also, the fiber can be separated and recovered. Oil and protein may be separated and recovered as well.