A separation container for extracting a portion of a sample for use or testing and method for preparing samples for downstream use or testing are provided. The separation container may include a body defining an internal chamber. The body may define an opening, and the body may be configured to receive the sample within the internal chamber. The separation container may further include a seal disposed across the opening, such that the seal may be configured to seal the opening of the body, and a plunger movably disposed at least partially inside the internal chamber. The plunger may be configured to be actuated to open the seal and express the portion of the sample.

A separation container for extracting a portion of a sample for use or testing and method for preparing samples for downstream use or testing are provided. The separation container may include a body defining an internal chamber. The body may define an opening, and the body may be configured to receive the sample within the internal chamber. The separation container may further include a seal disposed across the opening, such that the seal may be configured to seal the opening of the body, and a plunger movably disposed at least partially inside the internal chamber. The plunger may be configured to be actuated to open the seal and express the portion of the sample.

A portable apparatus useful for collection and processing of human biological material, such as adipose or cancellous bone material, to prepare a concentrated product (e.g., stromal vascular fraction). The apparatus has a container with a containment volume with a tissue retention volume and a filtrate volume separated by a filter and with a pellet well for collecting concentrate product in the form of a pellet phase from centrifuge processing. The pellet well is accessible only from above when the apparatus is in an access orientation. Collected pellet phase material may be removed from the pellet well by direct aspiration, without suspending the material in a suspension liquid within the container. Access ports may be configured for access only from above the container. The apparatus may include a tissue collector disposed in the disuse retention volume to engage and collect collagen or other stringy tissue. A method of processing adipose tissue to concentrate leuko stromal vascular cells includes multi-step processing using a portable container.

Methods are provided for extracting bone marrow cells from bone obtained from deceased donors, for preparing the bone marrow for cryopreservation and for obtaining desired cells from cryopreserved and fresh bone marrow.

An automatic cell isolation and collection system for sorting out target cells from a sample includes a crush module, a centrifuge module, a transport module, and a control unit. The centrifuge module includes centrifuge tubes and a magnetic mechanism providing a magnetically attractive force to at least one of the centrifuge tubes. The control unit electrically communicates with the crush module, the centrifuge module, and the transport module, and controls operation of the crush module, transport of the sample via the transport module after the sample is crushed by the crush module, centrifugation of the centrifuge module, and operation of the magnetic mechanism for providing the magnetically attractive force.

A centrifuge device is provided for the sizing and separation of constituents of a biologic mixture, e.g., adipose tissue. The device provides for the mechanical breaking down of the fibrous structure in the tissue by centrifugation causing the tissue to pass through a mesh element, or a sizing helix, or an extrusion element, whereupon the material is reduced to a slurry. This processed material may then be separated by centrifugation into its constituents, in order to harvest the fraction containing the multipotent cells. These multipotent cells may be utilized for various medical procedures to stimulate healing and tissue regeneration.

A stem cell culturing method includes a second bone marrow aspirate cultured along with a culture medium with first stem cells being fixed on a first culturing vessel bottom surface. When a total surface area of the first stem cells reaches a first target ratio with respect to the bottom surface area of the first culturing vessel, the first stem cells are extracted from the first culturing vessel. Top layer second stem cells are extracted from the first stem cells that are separated into layers and the second stem cells are cultured along with a culture medium. The second stem cells are fixed on a second culturing vessel bottom surface and, when a total surface area of the second stem cells reaches a second target ratio with respect to the bottom surface area of the second culturing vessel, the second stem cells are extracted from the second culturing vessel.

A system and method for automatically digesting, culturing and growing a biopsy. The digester is a closed fluid transfer system, including disposable and durable parts, that receives a biopsy, washes the biopsy tissue, digests the tissue to separate the cells from each other, and seeds the cells in a culture flask. In one use, the system receives cells from a patient, expands and multiplies them, and returns them to the patient. A controller coordinates the motion of a plurality of pumps and the status of a plurality of valves, the pumps and the plurality of valves moving the fluid throughout the system. A sensor system assists the controller in volume and flow rate control of the fluids in the system, and in controlling the position of a tube providing air and fluids to a biopsy vial that includes the biopsy.

Disclosed is an integrated urine anaerobic collection and culture apparatus, comprising: a catheter, at a front end of which a circular arc-shaped guide part and a first through hole are arranged, where a piston part is arranged inside the front end of the catheter; a first branch tube and a second branch tube, which are connected with the catheter, the first branch tube is connected with a first negative pressure vessel, and the first branch tube and the second branch tube are respectively provided with a first valve and a second valve; an aseptic centrifuge tube, which includes a tube body and a first cover body, where the first cover body is provided with a first check valve and a second check valve; a second negative pressure vessel. The present disclosure can realize the whole anaerobic operation of urine collection and culture in the bladder.

A method of preparing a fat graft includes introducing fat tissue into an internal chamber of a vessel of a cell or tissue isolation, purification, and storage device through a first opening of the vessel. The method also includes introducing an amount of a wash solution effective to wash the fat graft or an amount of a cryoprotectant effective to cryopreserve the fat graft into the internal chamber of the vessel through a second opening of the vessel and mixing the fat tissue with the wash solution or the cryoprotectant. The method further includes either: when wash solution is introduced, centrifuging the device containing the fat graft to separate the fat graft from the wash solution and drawing the wash solution from the first end of the device; or, when cryoprotectant is introduced, cooling the fat graft to a temperature below 0? C.