Process for carrying out an in vitro biochemical reaction for preparing a cell suspension suitable for application to the skin of a patient.

A steam separation unit for separation of steam from lignocellulosic biomass material in a hydrolysis process includes a treatment vessel including a separation section and a biomass collection section. The separation section is arranged with at least one inlet for receiving at least partly hydrolyzed biomass material mixed with steam and at least one control outlet for discharging the steam from the vessel. A biomass collection section arranged to be filled at least partly with liquid during operation and coupled to the separation section to collect the biomass material after separation from steam, wherein the biomass collection section includes a mixing element for mixing the biomass material with liquid and at least one control valve for discharging biomass material mixed with liquid. A system for hydrolysis of lignocellulosic biomass material including such a separation unit is also presented.

Anaerobic digestion apparatus comprises a first chamber for retaining organic matter before and/or during anaerobic digestion and a second chamber for retaining organic matter during anaerobic digestion. The anaerobic digestion apparatus is configured to refrigerate or heat the first chamber to suppress methanogenesis in the first chamber. The anaerobic digestion apparatus comprises a controller programmed to regulate the anaerobic digestion process and to thereby reduce system perturbations. The flow of organic matter to the second chamber where methanogenesis is regulated. There is disclosed an inoculum for anaerobic digestion comprising Acetobacterium woodii and Methanosaeta concilii.

A renewable energy microgeneration apparatus is disclosed that includes a mixing tank that mixes waste with a liquid, a buffer tank that receives and pre-warms the mixed waste, a pasteurization tank that pasteurizes on the pre-warmed mixed waste, a digestion tank that performs anaerobic digestion on the pasteurized waste, a de-watering device that separates liquid digestate and removes salt from the liquid, sensors that measure salinity and biogas quality, and a controller. The controller causes the transfer of digestate from the digestion tank to the pasteurization tank to the dewatering device, causes the de-watering device to separate the liquid and remove the salt from the liquid, monitors the salinity of the liquid and the quality of biogas using the sensors, and causes the mixing of the liquid with the waste and adjusts the feed rate of the waste to reduce the salinity of the waste and increase methane production.

There is disclosed a method for preparation of substrate for use in anaerobic digestion of organic waste. The method can be executed in a substrate pretreatment system, the method comprising: loading of a substrate into the substrate pretreatment system, mixing the substrate with liquid; heating the substrate and liquid to a pre-determined processing temperature; exposing the mixture to radiation; transferring the processed substrate to a bioreactor, during the heating, the method further comprises: degassing the mixture using vacuum extraction; and circulating the mixture past an ultrasonic hydrodynamic radiator.

A thermal pretreatment method for organic solid waste based on forced hot air convection is performed as follows. Experimental materials are grouped into an experimental group and a control group. The experimental group is subjected to thermal pretreatment in a thermal pretreatment device, and then removed. The experimental group and the control group are subjected to enzymatic hydrolysis and physicochemical characterization, and the analysis results are compared. A thermal pretreatment device for organic solid waste based on forced hot air convection is also provided.

A particulate carbon adsorbent for use in anaerobic digestion is provided. The particulate carbon adsorbent is substantially planar and comprising between 40-90 wt % carbon. Methods of manufacture of the particulate carbon adsorbent are also provided.

An organic waste digester system is provided. The system includes a heated hopper unit within the housing to receive organic waste. An agitation mechanism mixes the organic waste along with a microbe mixture to aid breakdown of the waste. Liquefied organic waste is discharged through an outlet and conveyed to a drying unit downstream of the hopper unit. The drying unit includes a microwave dryer unit.

The present invention relates to a process for production of fermentation products, including bioethanol by non-pressurised pre-treatment, enzymatic hydrolysis and fermentation of waste fractions containing mono- and/or polysaccharides, having a relatively high dry matter content. The process in its entirety, i.e. from non-pressurised pre-treatment over enzymatic hydrolysis and fermentation to sorting of fermentable and non-fermentable solids can be processed at a relatively high dry matter content in a single vessel or similar device using free fall mixing for the mechanical processing of the waste fraction.

A method of producing a sugar liquid from cellulose-containing biomass includes (1) to (3): (1): subjecting a cellulose-containing biomass to hydrothermal treatment and thereafter separating the treated cellulose-containing biomass into a hydrothermally-treated liquid and a cellulose-containing solid content; (2): adding a filamentous fungus-derived cellulase to the cellulose-containing solid content obtained in (1) to hydrolyze the cellulose and thereafter separating the hydrolysate into a saccharification residue and a sugar liquid; and (3): washing the saccharification residue obtained in (2) with the hydrothermally-treated liquid obtained in (1) to elute the filamentous fungus-derived cellulase adsorbed to the saccharification residue into the hydrothermally-treated liquid and thereafter obtaining a solution component comprising the filamentous fungus-derived cellulase by solid-liquid separation.