A device for blood (1) is provided with a column (50) and a micro flow path (20) located downstream of the column (50). The column (50) includes a porous material as a solid phase, and blood that has contacted with the porous material flows through the micro flow path (20). In the device for blood (1), the column (50) and the micro flow path (20) are provided as separated bodies. The column (50) has a connecting part (55), the micro flow path (20) has an inlet (21a), the connecting part (55) and the inlet (21a) are connected to each other to integrate the column (50) with the micro flow path (20), and blood (BL) is allowed to pass from the column (50).

The present invention provides compositions, systems, kits, and methods for analyzing the interaction of T-cells and neoantigen presenting cells (and other cells) via discrete entity (e.g., droplet) microfluids. In certain embodiments, a microfluidic device is used to merge a discrete entity containing a T-cell, and a discrete entity containing a neoantigen presenting cell, at a merger region via a trapping element in order to generate a combined discrete entity. In particular embodiments, at least one thousand of such combined discrete entities are formed in about one second. In some embodiments, whether the receptor on the T-cell sufficiently binds the neoantigen to activate the T-Cell is detected (e.g., via detection of cytokine or granzyme B release). In certain embodiments, provided herein are methods for identifying polyfunctional T-cells or NK-cells, as well as methods of screening for such cells that would be cytotoxic if injected into a subject.

A method for separating cells in a biofluid includes pretreating the biofluid by introducing a predetermined amount of a cocktail of antibodies, flowing the pretreated biofluid through a microfluidic separation channel, and applying acoustic energy to the pretreated biofluid within the microfluidic separation channel. A system for microfluidic cell separation, capable of separating target cells from non-target cells in a biofluid includes at least one microfluidic separation channel, a source of biofluid, a source of an additive including the cocktail of antibodies, and at least one acoustic transducer coupled to the microfluidic separation channel. A kit for microfluidic cell separation is also disclosed. A method of facilitating separation of cells is also disclosed.

The present invention is directed to systems and devices that allow for separation of cells based on size and electric properties and for high-throughput cell sorting. The system may comprise a microfluidic platform having a main microfluidic channel and cavity acoustic transducers (CATs). The microfluidic platform may be coupled to an external acoustic source. The system may further comprise a fluid disposed through the main microfluidic channel comprising cells having different sizes and electric properties. The fluid may intersect the CATs to form one or more interfaces. The system may further comprise electrodes underneath the microfluidic platform. The CATs may oscillate the interfaces to produce one or more microstreaming vortices, such that each microstreaming vortex is capable of selectively trapping cells based on size. The set of electrodes may apply an AC to cause the cells to move relative to the set of electrodes based on electric properties.

Microparticles and nanoparticles made of various materials that are used in various configurations are disclosed. The particles may be perfluorocarbon droplets with a lipid coating. The particles may be used in an acoustic cell selection process. The droplets are highly acoustically responsive and can be retained against fluid flow by an acoustic field. Such particles can be used in the separation, segregation, differentiation, modification or filtration of a system.

A workstation for processing particles entrained in a fluid includes a consumable portion and a reusable portion. The consumable portion is mounted to the reusable portion to form a fluid chamber in which an acoustic wave can be generated. The consumable portion implements a closed, isolated fluid environment that is managed using components of the reusable portion, such as valves, sensors and pumps. The workstation can be operated to retain particles from the fluid via the acoustic wave and provide a new fluid media to the retained particles. Following processing, the consumable portion can be removed and discarded.

A cell purification module, configured to purify multiple cells from a fluid sample is provided. The cell purification module includes a hollow column, multiple hollow fiber membranes, at least one first magnetic component, a fluid sample inlet end, and a fluid sample outlet end. The hollow column has a first opening, a second opening, and an accommodating space connecting the first opening and the second opening. The hollow fiber membranes are disposed in the accommodating space and each hollow fiber membrane has multiple pores. The first magnetic component is disposed at a periphery of the hollow column. The fluid sample inlet end and the fluid sample outlet end are respectively disposed at two ends of the hollow column. The hollow fiber membranes extend in an axial direction of the hollow column, and are arranged in a radial direction of the hollow column. A cell purification system is also provided.

An object of the present invention is to provide a separation substrate having a high megakaryocyte blocking rate and a high platelet permeation rate, and a cell separation filter and a method for producing a platelet which use the same. The separation substrate of the present invention is a separation substrate including non-woven fabric for separating a platelet from a cell suspension containing a megakaryocyte and the platelet, in which an average pore diameter of the separation substrate is 2.0 μm to 15.0 μm, and a thickness of the separation substrate is 10 μm to 500 μm.

A system for selective microcapsule extraction includes a non-planar core-shell microfluidic device. The non-planar core-shell microfluidic device generates microcapsules defining a core-shell configuration. A subset of the microcapsules contain aggregates, tissues, or at least one cell. A camera captures images of the microcapsules. A detection module includes a processor and a memory. The memory includes instructions that when executed by the processor causes the detection module to provide the images of the microcapsules as an input to a machine learning model. The machine learning model identifies microcapsules containing aggregates, tissues, or at least one cell. A force generator generates a force to extract the microcapsules. A microcontroller selectively activates the force generator to generate the force when the detection module identifies a microcapsule containing aggregates, tissues, or at least one cell to extract the microcapsule.

In order to cut a plurality of clumps having an approximately uniform shapes and approximately uniform dimensions out of a cell aggregate which has proliferated and appropriately eliminate contamination with fragments of different shapes or dimensions, when cutting the clumps of approximately uniform shape out of the cell aggregate which has proliferated, cutting lines along which the clumps of a specific shape are cut out are set such that the area of a peripheral part of the cell aggregate which is not cut by the cutting line exceeds the surface area of one of the clumps, and the cell aggregate is cut by irradiating with laser light in such a way as to trace the cutting lines.