A sonicator system for sonicating materials in a sample array includes a transducer layer configured to emit acoustic energy; and a multifocus acoustic lens layer configured to focus the acoustic energy from the transducer layer to the sample array to thereby simultaneously sonicate materials in the sample array.

An apparatus, multi-well plate and method for automated cell lysis and nucleic acid purification and processing. The plate includes a lysis well, at least one wash well, and an elution well. The apparatus includes a vertically aligned rotor mixer comprising a magnetic tip and actuators for moving the rotor mixer in a vertical and horizontal directions, to transfer magnetic beads from well to well. The rotor mixer is used to vortex lysis mixtures, wherein the vortexing speed is sufficient to overcome the magnetic attraction between the beads and mixer tip and disperse the beads in solution, to collect nucleic acids such as DNA.

Some embodiments are directed to a method for tracking lysis progress in an automated lysing device. For example, the lysing device may be configured to execute multiple iterations of lysing at a defined location on the tissue section and imaging the defined location on the tissue section using a camera. From the images a lysing parameter may be determined to improve lysing.

Lysis apparatus including a chamber for receiving a sample and at least one magnetic actuator located within the chamber as well as at least two magnetic elements arranged outside the chamber. Above that, such a lysis apparatus includes a driving unit for effecting a rotational relative movement between the chamber and the at least two magnetic elements arranged outside the chamber, wherein the polarity of the magnetic elements is opposite with respect to the circular path of the rotational relative movement and hence, for example, the chamber, such that the magnetic actuator arranged within the chamber is moved both translationally and rotationally to effect lysis of the sample. Here, the chamber is configured, e.g., by its dimensioning or a flexible outer shell, to enable the at least one magnetic actuator located within the chamber to move both translationally and rotationally.

The embodiments according to the present disclosure provide a protective solution, a kit, and a method for isolating mitochondria. The kit includes an extraction tube, a protective solution, and a suction needle. The extraction tube is used to contain cells. The protective solution is used to mix with the cells in the extraction tube to form a mixture solution, and the osmolarity of the protective solution is greater than 0 and less than or equal to 220 mOsm/L. The suction needle is used to suck the mixed solution back and forth to facilitate the release of mitochondria from cells. This method and the protective solution may isolate mitochondria from cells with high efficiency in a simple and convenient way, and the isolated mitochondria have excellent good function.

According to present disclosure, there is disclosed an algae growth and cultivation system that provides a cost-efficient means of producing algae biomass as feedstock for algae-based products, such as, fertilizer, feed, biofuel manufacture, and desirably impacts, nutrient recovery from waste streams for valued byproducts production, recycle water, and alternative/renewable energy production. The system as discussed herein is an integrated systems approach to wastewater treatment, algal strains selection for byproducts production, and recycle of algal biomass-processing waste or additional algae harvested as feedstock for products such as fertilizer production. Embodiments of a system as discussed herein present an economically viable algae production system and process that allows algae-derived products such as fertilizer, feed, biofuels, etc. to compete with non-organic or petroleum products in the marketplace.

Provided are an oleaginous microorganism disruption process using a supersonic disperser and a method for producing bio-oil using the same. The method for producing bio-oil according to the present invention induces a cell disruption of oleaginous microorganisms without a separate drying process, thereby providing a method for continuously producing bio-oil in an economical and simple manner. In addition, the method of the present invention induces a cell disruption of oleaginous microorganisms without a heating process, thereby producing bio-oil without a change in physical properties due to the heat.

The present disclosure relates to the manufacture and use of redox electrodes and their use in cell lysis. In certain embodiments, the redox electrodes are manufactured using a hybrid material approach, such as using a redox polymer in combination with a support substrate, such as cellulose fibers or paper. In certain implementations, the redox electrodes are suitable for use at voltages greater than 25 Volts.

A process carried out for lysing biological species present in an aqueous solution and for recovering a biological material resulting from the lysis of the biological species, the process being carried out by using a system which includes a vessel delimiting a volume, a first electrode and a second electrode each including an end placed in the volume formed by the vessel, a voltage-pulse-generating circuit. The process includes applying at least one voltage pulse between the two electrodes with the voltage-pulse-generating circuit connected to the two electrodes, so as to bring about the creation of an electric arc between the end of the first electrode and the end of the second electrode and the generation of at least one shockwave in the solution.

An apparatus, vortex generator assembly and method for automated cell lysis and nucleic acid purification and processing. The vortex generator assembly includes sample holder having a lysis well, at least one wash well, and an elution well. The vortex generator assembly also includes a sample holder cover having a plurality of vibration rods for creating a vortex in the wells of the sample holder. The apparatus includes motor operating a rotating cam to cause the vibration rods to vibrate and create the vortex in a well holding fluid and magnetic beads, wherein the vortexing speed is sufficient to overcome the magnetic attraction between the beads and disperse the beads in solution, to collect nucleic acids such as DNA.