A homogenized and integrated device with a coaxial line and double-high pressure cylinder, includes a long oil cylinder, two main connecting sleeves, two high pressure cylindrical homogenized main bodies, two auxiliary connecting sleeves and two short oil cylinders. The two main connecting sleeves, two high pressure cylindrical homogenized main bodies, two auxiliary connecting sleeves and two short oil cylinders are respectively and symmetrically arranged at two ends of the long oil cylinder and are assembled with the long oil cylinder along a same axial line. Each high pressure cylindrical homogenized main body is integrally connected with the long oil cylinder by virtue of one of the main connecting sleeves. Each high pressure cylindrical homogenized main body is integrally connected with the corresponding short oil cylinder by virtue of one of the auxiliary connecting sleeves.

Systems and methods for the efficient agitation of tissue samples. A device may include a plurality of chambers that each receives samples therein. The plurality of chambers may be uniformly spaced with respect to a least one dimension, to form a one dimensional or two dimensional array. Each of the chambers may include an opening and an agitator device in fluid contact with the sample disposed within the chamber. The agitator devices may include a micromotor which provides rotational motion to a shaft and an impeller fixed to the shaft such that the impeller and the shaft rotate together upon provision of the rotational motion by the micromotor. The system may include an electrical energy source electrically coupled to the plurality of micromotors to rotate the impellers sufficient to agitate the sample as required for a particular activity (e.g., homogenization, lysis).

A method for purifying a target protein in high yield is disclosed. The target protein includes a membrane protein. The purification method optimizes crushing and elution conditions during processes of separation and purification of membrane proteins, and when using the method to purify membrane proteins, the membrane proteins can be purified in a higher yield that is not less than 100 times compared to conventional homogenizer or sonic pulverization process. In addition, the purification method render removals of nuclei, peroxisomes, and lysosomes, thereby reducing DNA contamination and protein damage by proteases.

Protein-rich nutrient supplements and animal feed supplements derived from an anaerobic bacterial process are generated through a myriad of cell rupturing and protein fractionation/purification processes. Bacterial fermentation systems and methods of obtaining one or more protein-containing portions from a fermentation process using carbon monoxide-containing gaseous substrates are provided. The invention further provides compositions of protein-rich nutrient supplements with useful applications for intake by a variety of different animals and humans.

Protein-rich nutrient supplements and animal feed supplements derived from an anaerobic bacterial process are generated through a myriad of cell rupturing and protein fractionation/purification processes. Bacterial fermentation systems and methods of obtaining one or more protein-containing portions from a fermentation process using carbon monoxide-containing gaseous substrates increasing protein product yield from bacterial cells are provided. The invention further provides compositions of protein-rich nutrient supplements with useful applications for intake by a variety of different animals and humans.

Protein-rich nutrient supplements and animal feed supplements derived from an anaerobic bacterial process are generated through a myriad of cell rupturing and protein fractionation/purification processes. Bacterial fermentation systems and methods of obtaining one or more protein-containing portions from a fermentation process using carbon monoxide-containing gaseous substrates are provided. The invention further provides compositions of protein-rich nutrient supplements with useful applications for intake by a variety of different animals and humans.

Protein-rich nutrient supplements and animal feed supplements derived from an anaerobic bacterial process are generated through a myriad of cell rupturing and protein fractionation/purification processes. Bacterial fermentation systems and methods of obtaining one or more protein-containing portions from a fermentation process using carbon monoxide-containing gaseous substrates are provided. The invention further provides compositions of protein-rich nutrient supplements with useful applications for intake by a variety of different animals and humans.

A method of producing a nutritional supplement from microbial cells. The method includes cultivating microbial cells to obtain a biomass; incubating the biomass with a heat treatment at temperature from 55? C. up to 80? C. for an incubation time from 10 minutes up to 60 minutes; concentrating the biomass by separating and removing a liquid phase from a solid phase to obtain a dry matter content from 2% up to 40% of total weight of the nutritional supplement. Disclosed also is a system of producing a nutritional supplement from microbial cells using the aforementioned method. The system includes a bioreactor; a heat-exchanger and a separator for carrying out the respective steps of the aforementioned method.

Reference is made to the Identification of the Microorganism, with the identification reference given by the Depositor SoF1, having the Accession number given by the INTERNATIONAL DEPOSITORY AUTHORITY, VTT E-193585, received on Jun. 11, 2019

Methods and devices are disclosed for processing stromal precursor cells (i.e., cells which can differentiate into connective tissue cells, such as in muscles, ligaments, or tendons) which can be obtained from fatty tissue extracts obtained via liposuction. Normal processing of a liposuction extract involves centrifugation, to concentrate the stromal cells into a semi-concentrated form called spun fat. That spun fat can then be treated by mechanical processing (such as pressure-driven extrusion through 0.5 mm holes) under conditions which can gently pry the stromal cells away from extra-cellular collagen fibers and other debris in the spun fat. The extruded mixture is then centrifuged again, to separate a highly-enriched population of stromal cells which is suited for injection back into the patient (along with platelet cells, if desired, to further promote tissue repair or regeneration).

Methods and devices are disclosed for processing stromal precursor cells (i.e., cells which can differentiate into connective tissue cells, such as in muscles, ligaments, or tendons) which can be obtained from fatty tissue extracts obtained via liposuction. Normal processing of a liposuction extract involves centrifugation, to concentrate the stromal cells into a semi-concentrated form called spun fat. That spun fat can then be treated by mechanical processing (such as pressure-driven extrusion through 0.5 mm holes) under conditions which can gently pry the stromal cells away from extra-cellular collagen fibers and other debris in the spun fat. The extruded mixture is then centrifuged again, to separate a highly-enriched population of stromal cells which is suited for injection back into the patient (along with platelet cells, if desired, to further promote tissue repair or regeneration).