The present disclosure relates to hollow fiber tangential flow filters, including hollow fiber tangential flow depth filters, for various applications, including bioprocessing and pharmaceutical applications, systems employing such filters, and methods of filtration using the same.

A perfusion bioprocessing system (10) includes a bioreactor (12) and a recirculation flow path (14) provided with at least in one first feed flow control device (46) and at least one second feed control device (48). The perfusion bioprocessing system (10) further includes a first tangential flow filter (16) coupled to the bioreactor (12) via the recirculation flow path (14) and a second tangential flow filter (18) coupled to the bioreactor (12) via the recirculation flow path (14). The first tangential flow filter and the second tangential flow filter are coupled to a permeate flow path and a retentate flow path. Additionally, the perfusion bioprocessing system (10) includes a control unit (90) coupled to the at least one first feed flow control device (38) and the at least one second feed control device (40).

The invention relates to the isolation or extraction of exosomes.

A hydrogen peroxide and gluconic acid production method and system is disclosed that can include receiving an aqueous solution having glucose, water, and glucose oxidase at a reaction chamber. Here, the reaction chamber facilitates an enzymatic reaction between a gas phase and a liquid phase of the aqueous solution, thereby yielding a first solution comprising hydrogen peroxide, gluconic acid, and the glucose oxidase. The method can further include receiving the first solution at a separation chamber, wherein the separation chamber is comprised of a semi-permeable membrane having a pre-defined molecular weight barrier for separating the glucose oxidase, thereby resulting in a combined hydrogen peroxide and gluconic acid solution. The method can further include at least partially converting the gluconic acid into a gluconate salt, and separating and concentrating the hydrogen peroxide from the gluconic acid or gluconate salt via vacuum flash evaporation and vacuum distillation.

The present invention relates to a large-scale magnetic purification system, including: a liquid storage apparatus, capable of being connected to a liquid source and a waste liquid barrel through a liquid path system; a stirring system mounted above the liquid storage apparatus, where a stirring paddle of the stirring system is inserted into the liquid storage apparatus for stirring; magnets mounted around the liquid storage apparatus; a magnet actuating mechanism for manipulating the magnets to move away from or close to the liquid storage apparatus; and a control system, connected to the liquid path system, the magnet actuating mechanism, and the stirring system and controlling the liquid path system, the magnet actuating mechanism, and the stirring system.

Systems and methods for containing and manipulating liquids, including vessels and unit operations or components of cell culture, cell containment, bioreactor, and/or pharmaceutical manufacturing systems, are provided. In certain embodiments, such vessels and unit operations are directed to continuous perfusion reactor or bioreactor systems and may include one or more disposable components. For instance, in one aspect, a system includes an apparatus in the form of a bioreactor for harvesting cells which produce one or more products. The apparatus may include a disposable, collapsible bag adapted for containing a liquid, the collapsible bag in fluid communication with a liquid-solids (e.g., cell) separation device. For example, an outlet of the collapsible bag may be connected to an inlet of the separation device, and an outlet of the separation device may be connected to an inlet of the collapsible bag for recycle. Accordingly, after separating the cells from the liquid in the separation device, the cells can be flowed back into the collapsible bag where they can be re-harvested. Meanwhile, product contained in the liquid can be collected in a separate container. The efficiency of product formation in such a system may be enhanced by using mixing systems described herein.

The subject invention provides methods and systems for producing microbe-based compositions that can be used in the oil and gas industry, environmental cleanup, agriculture, and many other applications. More specifically, the subject invention provides methods and systems for producing microorganisms and/or growth by-products thereof using a hybrid solid state-submerged fermentation method, wherein a matrix comprised of a solid material covered in an alginate coating is formed inside a liquid fermentation vessel. In some embodiments, the solid material is a plurality of natural loofa sponges.

Filter elements for perfusion systems and methods are provided. A filter element sheet includes a microporous membrane having a mean pore size of at least about 0.65 μm and a feed spacer comprising woven fibers and having an open area of at least about 35%. The filter element sheet can be arranged within a filter element, for example, in a spiral-wound format or in a cassette format. A perfusion system includes at least one filter element and a pump configured to control flow of a liquid feed through the at least one filter element.

Through sourcing net-primary productivity additive algae-based biomass feedstock, the exclusive use of renewable energy in processing, and the appropriate formulation and processing, a novel algae-derived bio-based plastic is both carbon-negative and provides some performance advantages over existing algae-based film plastics especially with regard to optical clarity. A system may be provided that produces a carbon-negative bioplastic. The production of the bioplastic in a process chamber may be controlled by an electronic controller. The electronic controller may be controlled by a host system, such a server. The electronic controller may be configured to direct production of the bioplastic in the process chamber using hydrocolloid, which is derived from algae.

Spent stillage remaining after the fermentation of a feedstock for ethanol production may be processed to recover, use, and/or recycle the constituent components of the stillage. Stillage may be mixed, heated, and held at a desired temperature for a period of time. The stillage may then be cooled and treated with an enzyme. The enzymatically treated stillage may be emulsified with oil and water, and then permitted to settle into discrete layers. Individual layers may then be processed. A system and method for separating stillage after the fermentation process of ethanol production has concluded are provided.