A filtration system for downstream processing of a suspension containing a labile solid material in a liquid medium, wherein dissolved gas in liquid environment is controlled to ensure optimal process conditions. The dissolved gas can be controlled by a gas-liquid interface by bubbling; a liquid-liquid interphase transfer process using a gas vector substance; a liquid-solid interphase transfer process using a gas permeable solid or a reaction evolving oxygen or other gas/gases. The retained solid material is removed in batch operations, continuous operations or mixed-type operations. The process can also include further filtration, heat-treatment, and removal of at least a part of the liquid. The filtration system could also process a concentrated suspension which is devoid of the free liquid, then heat-treated and the liquid produced by the treatment removed by further filtration. The solid material can be a biomass.

The invention includes novel, systems, methods and compositions for the in vitro production of polynucleotides, and in particular the production of mRNA for use in therapeutic applications.

The present invention relates to cell free protein manufacturing, and more particularly, for integrating machine learning into a portable cell-free bioprocessing system for producing proteins with increased and consistent purity, potency and quality wherein such proteins are prepared on-demand and for point-of-care delivery.

The invention concerns an installation for treatment of biological liquid by chromatography, extending in a longitudinal direction and comprising a supply valve (20b), a supply pump (30) downstream of the valve, instrument members downstream of the pump including distribution valves (81a-c, 82a-c, 83a-c) and devices (78a-c, 85a-c, 86a-c) for measuring physico-chemical parameters of the liquid, chromatography columns (99a-c) downstream of the instrument members and pipes connecting the valve, the pump, the instrument members and the columns so as to form at least one supply line for biological liquid to treat of a treatment circuit of said installation, the chromatography columns being disposed relative to each other in a direction of extension generally transverse to said generally longitudinal direction of extension of the installation.

Viral filters include a filter member featuring a first surface and a second surface and having a thickness extending between the first and second surfaces in a first direction, and a plurality of channels formed in the filter member, each of the channels having a channel axis, where during use, a solution carrying a viral load flows in a direction parallel to the first surface, and at least a portion of the viral load enters the membrane through the first surface and propagates in the first direction, and where for at least 50% of the channels in the filter member, the channel axis is oriented at an angle of between 5 degrees and 85 degrees relative to the first direction.

Provided herein is a column-based continuous viral inactivation system, comprising one or both of a pH feedback controller to adjust feed flow rates and a minimum residence time (MRT) feedback controller to adjust feed flow rates. Methods of viral inactivation with the system are also provided.

Container for sample preparation or processing, such as biomass culturing or processing, and optionally sample purification. In certain embodiments, the reactor is a bioreactor that includes a stirred cell device that simulates a tangential flow filter to reduce or eliminate clogging that can be caused by the solids generated. In certain embodiments, the solids comprise a precipitate or floc or beads, such as one that includes a polymer that binds the biomolecule(s) of interest, and impurities. In its method aspects, embodiments disclosed herein include purification and isolation of biomolecules of interest derived from cell culture fluids. The methods include carrying out sample preparation or processing in a container, culturing a biomass; generating solids by precipitating or flocculating a biomolecule of interest from the cultured broth; preventing the solids from settling in the container by agitation; and purification, such as by eluting the biomolecule of interest and filtering the same.

The present disclosure describes a DNA fragment mixture for the prevention or for the treatment of at least one pathogenic, parasitic or infesting species of plants or of the environment, wherein the DNA mixture consists of random fragments of total DNA of at least one pathogenic, parasitic, or infesting species, and/or at least one phylogenetically similar species, against which the prevention and treatment are directed. Further, the disclosure describes a process and related system for improvement of the production/growth of microorganisms at high yield in bioreactors or photobioreactors, or of plants in different culture systems, where the nucleic acids of the organisms produced/grown by such a process are removed from the culture medium and the culture medium, deprived of these nucleic acid, is used again in the process.

An automatic purification system using magnetic particles comprises: 1) a first container module; and 2) a system controller module. The first container module comprises a first container and a first magnetic field supply device disposed outside the first container. A first container liquid inlet is formed in the upper portion of the first container, and a first container liquid outlet is formed in the bottom of the first container. The system controller module can generate a variable magnetic field in the first container by controlling the first magnetic field supply device. A method for purifying a target component from a biological sample comprises a step for allowing a biological sample containing a target component to be in contact with magnetic particles capable of specifically binding the target component, in the first container in the automatic purification system. The automatic purification system and method help to efficiently separate a target product from a biological sample, and helps to reduce process costs and time at the same time.

A tissue processing apparatus, a filter and a method for processing tissue therefrom #! The problem to be solved is to provide a portable apparatus for processing harvested fat to form a good quality graft that does not clog syringes, and the problem is solved by providing an apparatus as in the present invention with a filter that is inclined to the base of the apparatus and comprises of plurality of elongated protrusion that are structured at an angle on the surface of the filter and blocks the fibres when the harvested fat moves on the inclined surface of the filter, thereby filtering the harvested fat and avoiding clogging of syringes.