A system includes an algae bioreactor that contains an algae slurry, a heat exchanger in fluid communication with the algae bioreactor to receive the algae slurry from the algae bioreactor and heat and increase a pressure of the algae slurry, and one or more valves and a flash vessel in fluid communication with a discharge of the heat exchanger to flash the algae slurry and create steam and algae biomass. A separator receives the algae biomass from the flash vessel and separates oils from the algae biomass to generate a biofuel.

A kit for magnetic cell isolation includes first stopcock manifold having at least four stopcocks, a separation chamber configured for use with a centrifugal processing chamber of the cell processing device, the separation chamber in fluid communication with the first stopcock manifold, a mixing bag configured for use with a heating/cooling mixing chamber of a cell processing device, the mixing bag in fluid communication with the first stopcock manifold, a second stopcock manifold having at least four stopcocks, the second stopcock manifold in fluid communication with the first stopcock manifold, a magnetic cell isolation holder in fluid communication with the second stopcock manifold, the magnetic cell isolation holder configured for use with a magnetic field generator of a magnetic cell isolation device, and a plurality of cell processing bags in fluid communication with the first and/or second stopcock manifolds.

Provided are a cell dispensing and storing apparatus and a method of the same. The method includes: moving a freezing container with a test tube in it and a temporary storage bottle containing sample cells into a dispensing area. Connect the temporary storage bottle with an injecting assembly. A dispensing clamp moves the test tube from the freezing container to a tube rack on a rotating platform. A rotating clamp removes a tube cover from the test tube. An injecting nozzle extracts the sample cells from the temporary storage bottle and dispenses the sample cells into the test tube. The rotating clamp puts the tube cover back on the test tube. The dispensing clamp moves the test tube back into the freezing container. A freezing clamp moves the freezing container into a freezer.

Disclosed is a mass-cultivation system for microalgae, including a reactor that contains a cultivation liquid in the interior thereof, wherein the liquid includes functional particles. According to the mass-cultivation system for microalgae according to the present invention, because various functions that are necessary for cultivation of microalgae may be uniformly distributed in a cultivation liquid by allowing functional particles having various functions to flow in the cultivation liquid, a suitable environment may be created based on the cultivation of a large amount of microalgae and the growth of microalgae so that a high efficiency cultivation system may be realized while the problems of mass-cultivation of an existing cultivation system may be solved.

Spent stillage remaining after the fermentation of a feedstock for ethanol production may be processed to recover, use, and/or recycle the constituent components of the stillage. Stillage may be mixed, heated, and held at a desired temperature for a period of time. The stillage may then be cooled and treated with an enzyme. The enzymatically treated stillage may be emulsified with oil and water, and then permitted to settle into discrete layers. Individual layers may then be processed.

The present disclosure features methods and compositions for increasing the amount of products of cellular metabolism, e.g., proteins, by lowering the temperature of cells expressing the product at one or more steps while culturing the cells, expressing the product, and/or recovering the product.

Application of the present invention enables quantification of fractions of candidate pharmaceutical compounds (a parent compound and its metabolites), one excreted to the basolateral (Basal/Basolateral)-side via transporters and by diffusion, one excreted to the lumen (Apical)-side, and one remained in the cells. This enables determination of the total amount of the administered candidate pharmaceutical compounds and the distribution ratio of the fractions. The kinetics of the administered candidate pharmaceutical compounds can be evaluated, thereby enabling in vitro screening of an enormous number of candidate pharmaceutical compounds for drug candidates exhibiting the efficacy. The object of the present invention is to provide an apparatus and method for understanding a total picture of pharmacokinetics in vitro by quantifying a fraction of basolateral (Basal/Basolateral) efflux, a fraction of lumen (Apical)-side excretion, and a fraction remaining in a cell of a drug which has been administered to the cell to determine the distribution ratio of each fraction.

A flowcell device, including a flow pathway and an optical subassembly, has a flowcell body that is continuous with a sample being analyzed and a temperature controlled surface. The flowcell body can be disposed between a thermalplate, actively regulated by a thermoelectric cooler, and an insulating member. The flowcell device can be employed in a bioreactor monitoring system.

This patent document relates to the field of biotechnological apparatuses. It relates particularly to a biotechnological apparatus comprising a bioreactor, an exhaust gas temperature control device and a method for treating an exhaust gas stream. This patent document further relates to a connection device for a sterile single-use fluid conduit of a single-use bioreactor and to a method for treating a fluid stream. The reusable connection device comprises a receptacle in which a portion of the fluid conduit can be detachably arranged, and a coupling member connecting the connection device to a temperature control device, the receptacle having a contact surface which is arranged such that the contact surface abuts a fluid conduit arranged in the receptacle, and wherein a portion of a fluid conduit can be introduced into the receptacle in a direction running substantially orthogonally to a longitudinal axis of the connection device.

Application of the present invention enables quantification of fractions of candidate pharmaceutical compounds (a parent compound and its metabolites), one excreted to the basolateral (Basal/Basolateral)-side via transporters and by diffusion, one excreted to the lumen (Apical)-side, and one remained in the cells. This enables determination of the total amount of the administered candidate pharmaceutical compounds and the distribution ratio of the fractions. The kinetics of the administered candidate pharmaceutical compounds can be evaluated, thereby enabling in vitro screening of an enormous number of candidate pharmaceutical compounds for drug candidates exhibiting the efficacy. The object of the present invention is to provide an apparatus and method for understanding a total picture of pharmacokinetics in vitro by quantifying a fraction of basolateral (Basal/Basolateral) efflux, a fraction of lumen (Apical)-side excretion, and a fraction remaining in a cell of a drug which has been administered to the cell to determine the distribution ratio of each fraction.