Production of nutrient-rich media, from an initial minimal medium, the rich media being suitable for cultivating heterotrophic cells, is described. These methods employ gas fermentation of photoautotrophic and/or chemoautotrophic microbes, under chemoautotrophic conditions, using carbon in common industrial waste gases to feed the growing biomass. The microbes also transform some of the carbon into organic nutrients that are released into the minimal medium thereby enriching the minimal medium. In further methods the nutrient-rich medium is then used to cultivate heterotrophic cells.

The present disclosure relates to a method for preparing killed lactic acid bacteria using a bioreactor including a culture device and a membrane filter, and to killed lactic acid bacteria prepared by the preparation method.

Provided are a method of decolorizing a composition including pectate lyase, and a decolorizing composition for the composition including pectate lyase.

A method for producing a basic acid such as L-lysine is provided. A basic amino acid or a fermentation product containing the same is produced by a method including the following steps (A) and (B): (A) a step of culturing a microorganism able to produce a basic amino acid in a culture medium so that bicarbonate ions and/or carbonate ions serve as counter ions for the basic amino acid, to obtain a fermentation broth containing the basic amino acid; (B) a step of subjecting the fermentation broth to a heat treatment under a pressure sufficient for preventing generation of carbon dioxide gas from the fermentation broth.

Food products are provided in which microbial protein sources, such as single cell protein or protein hydrolysis products, are incorporated, and from which endotoxins, and optionally nucleic acids, have been reduced or removed.

The invention concerns a process for deactivating a cellulolytic microorganism enabling the production of an enzymatic cocktail, said cocktail being used without separating the cellulolytic microorganism during the biochemical conversion of lignocellulosic materials, comprising at least one step for bringing a gaseous stream into contact with a medium containing said microorganism, said gaseous stream comprising more than 25% by weight of CO.sub.2 and comprising less than 0.5 molar % of O.sub.2.

The present invention provides a method of controlling bacterial contamination using synergistic interactions of antimicrobials. The invention consists of combinations of chlorine dioxide and organic acid whose combined antimicrobial effect is greater than the sum of their individual activities, i.e., synergistic.

A method of obtaining and producing an antimicrobial bioactive extract from nitrogen stress-grown microalgae is provided. An antimicrobial activity study is carried out with the antimicrobial bioactive extract obtained from the microalgae genus Auxenochlorella protothecoides.

A method for concentrating, in order to favour the subsequent extraction process of intracellular lipids, a cell suspension containing a biomass of oleaginous yeasts fermented in fermentation broth under conditions that allow the intracellular accumulation of lipids, where the biomass contains significant quantities of mucilaginous material. The method includes:

a) cultivating the oleaginous yeasts in a fermentation broth to obtain a cell suspension containing the mucilaginous biomass;

b) subjecting the cell suspension obtained from a) to heat treatment, at a temperature between 95 C. and 120 C. and to acid treatment, to obtain a treated cell suspension containing the mucilaginous biomass containing intact oleaginous yeast cells; and

c) concentrating the treated cell suspension obtained from b), by removing at least part of the fermentation broth to obtain a concentrated cell suspension.

The present invention relates to a method for optimising the downstream processing of a protein-rich microalgae biomass of the Chlorella genus previously prepared by fermentation in heterotrophic conditions and in the absence of light, comprising: 1) providing biomass comprising more than 50% protein by dry weight of biomass; next, at low temperature, carrying out the following steps: 2) harvesting the biomass at the end of fermentation, 3) washing and concentrating the biomass, 4) optionally, lysing the biomass, next, without low temperature stress, 5) optionally, concentrating the biomass slurry, 6) applying heat treatment, 7) drying the biomass obtained in this way in order to obtain the product, a step of adjusting the pH to 7 being applied before or after the heat treatment step 6).