Method for removing all types of particulate matter with exclusion of biological multiplying microorganisms in indoor environments characterized by comprising the following two steps:the atomization of compounds that oxidize particulate matter to form complexes and to precipitate them;the atomization of a liquid containing a mixture of spores of Gram-positive aerobic and/or of facultative anaerobic spore formers to act as nuclei for extra precipitation, to cover the precipitated particulate matter; and to metabolize precipitated particles and to take up precipitated particles by the Gram-positive bacteria/thereby preventing the precipitated particles to become airborne again;whereby the drop size in both atomization steps is held between 5 and 50 m to yield a dry nebula.

In place of a step of centrifugation during preparation of outer membrane vesicles (OMVs) from bacteria, the invention utilises ultrafiltration. This allows much larger amounts of OMV-containing supernatant to be processed in a much shorter time. Thus the invention provides a process for preparing bacterial OMVs, comprising a step of ultrafiltration. The ultrafiltration step is performed on an aqueous suspension of OMVs after they have been prepared from bacteria and the OMVs remain in suspension after the filtration step. The invention is particularly useful for preparing OMVs from Neisseria meningitidis.

A method for deep dehydration and desiccation of cyanobacteria includes steps of flocculation conditioning, high pressure diaphragm plate-frame pressure filtration, quartz glass box-type desiccation and negative pressure paddle drying.

In place of a step of centrifugation during preparation of outer membrane vesicles (OMVs) from bacteria, the invention utilizes ultrafiltration. This allows much larger amounts of OMV-containing supernatant to be processed in a much shorter time. Thus the invention provides a process for preparing bacterial OMVs, comprising a step of ultrafiltration. The ultrafiltration step is performed on an aqueous suspension of OMVs after they have been prepared from bacteria and the OMVs remain in suspension after the filtration step. The invention is particularly useful for preparing OMVs from Neisseria meningitidis.

According to the present invention, a powder containing a carotenoid for feed having improved color enhancing ability, and a method for producing the same are provided. A method for producing a dried bacterial cell powder containing a carotenoid, comprising a step of drying via conductive heat transfer and a pulverization step and a dried bacterial cell powder produced by the method are provided.

A method for filtering a sample comprising the steps of: (a) providing a sample with eukaryotic cells and containing or suspected to contain a micro-organism; (b) performing a selective lysis of the eukaryotic cells to obtain a lysed sample; (c) filtering the lysed sample obtained in step (b) through a filter arranged to retain the micro-organism; and (d) washing the filter with a detergent based wash buffer to selectively solubilize proteins originating from the eukaryotic cells retained by the filter, by passing the detergent based wash buffer through the filter, to remove protein clogs from the filter in order to allow an additional step (c) of filtering said lysed sample.

An improved pasteurisation protocol for pasteurising microbial cells is disclosed. The protocol has three stages, a first heating stage, a second plateau stage at which the cells are held at a (maximum and) constant temperature, and a third cooling stage. Both the heating and the cooling stages are rapid, with the temperature of the cells passing through 40 to 80 C. in no more than 30 minutes in the heating stage. The heating rate is at least 0.5 C./minute and during cooling is at least 0.5 C./minute. The plateau maximum temperature is from 70 to 85 C. By plotting the pasteurisation protocol on a time (t, minutes) versus temperature (T, C.) graph, one obtains a trapezium having an area less than 13,000 C. minute. Not only does this result in a smaller energy input (and so a reduction in costs), but a better quality (and less oxidised) oil results having a peroxide value (POV) of less than 1.5 and an anisidine value (AnV) of less than 1.0.

Arginine deiminase (ADI)-containing genetically engineered Corynebacterium glutamicum (C. glutamicum), a fusion protein cipA-arc, use thereof, and a method for preparing [.sup.14/15N]-L-citrulline through enzymatic catalysis are provided. The ADI-containing genetically engineered Corynebacterium glutamicum (C. glutamicum) has a deposit number of CGMCC No. 19404, which expresses a fusion protein cipA-arc. Both the genetically engineered strain and the fusion protein cipA-arc can be used to convert [.sup.14/15N]-L-arginine into [.sup.14/15N]-L-citrulline.

By swinging a culture vessel before aspiration removal of a medium, a centrally dense state (83) of the cell population is formed. In the centrally dense state (83), a cell cluster (84) is separated from an outlet port (58a). After aspiration removal of the medium, a new medium is introduced into the culture vessel. After the introduction of the new medium, an overall dispersed state of the cell population is formed.

The present invention relates to a method for producing a microalgae concentrate with low viscosity, the method comprising a step of adjusting the pH of a microalgae fermentation broth and a heat treatment step, in which utility costs and investment costs can be reduced in a dehydration and drying step to secure price competitiveness for production and commercialization of dried microalgae products.