A system for quickly determining antibiotic sensitivity of a microorganism comprising a triangular-shaped plate and cover for culturing, recovering, and re-suspending recovered microbial colonies and a second triangular-shaped plate comprising a non-liquid medium cut with concentric trenches over which the re-suspended microbial colonies are distributed by centrifugation and contacted with antimicrobial strips or disks.

The present disclosure provides a method for extracting alpha-ketoglutarate and pyruvate simultaneously from microbial fermentation broth or enzyme transformation solution, which is related to the technical field of biological separation and extraction. The method comprises the following steps: centrifuging the microbial fermentation broth or enzymatic conversion solution containing α-KG and PA to remove the cells and other visible solids; removing the macromolecular impurities by ultrafiltration; evaporating and concentrating under reduced pressure conditions; extracting with the water-insoluble extraction after acidification; separating crude crystals of α-KG and crude liquid of PA by evaporation crystallization method (if concentration of PA is great higher than that of α-KG, crystallization separation should be conducted after distilling partial pure pyruvate); washing the crude crystal of α-KG with water-insoluble organic solvent as ethyl acetate or butyl acetate, drying and crushing to obtain qualified α-KG; distilling to gain qualified PA product applying high vacuum distillation (or molecular distillation).

The present disclosure provides a method for extracting alpha-ketoglutarate and pyruvate simultaneously from microbial fermentation broth or enzyme transformation solution, which is related to the technical field of biological separation and extraction. The method comprises the following steps: centrifuging the microbial fermentation broth or enzymatic conversion solution containing α-KG and PA to remove the cells and other visible solids; removing the macromolecular impurities by ultrafiltration; evaporating and concentrating under reduced pressure conditions; extracting with the water-insoluble extraction after acidification; separating crude crystals of α-KG and crude liquid of PA by evaporation crystallization method (if concentration of PA is great higher than that of α-KG, crystallization separation should be conducted after distilling partial pure pyruvate); washing the crude crystal of α-KG with water-insoluble organic solvent as ethyl acetate or butyl acetate, drying and crushing to obtain qualified α-KG; distilling to gain qualified PA product applying high vacuum distillation (or molecular distillation).

A therapeutic vaccination method includes growing and harvesting viruses, bacteria, fungi, parasites, or tumor cells on a cell culture or other appropriate medium; killing the viruses, bacteria, fungi, parasites, or tumor cells in the cell culture or other appropriate medium with a dose of methylene blue; separating the dead viruses, bacteria, fungi, parasites, or tumor cells from a remainder of the cell culture or other appropriate medium using a filter and/or centrifuge; adding antivirals, antibacterials, antifungals, antiparasitics, and/or anti-neoplastic medications at non-toxic therapeutic concentrations to the dead viruses, bacteria, fungi, parasites, or tumor cells so as to form a therapeutic vaccine; and administering the therapeutic vaccine to a patient in need thereof to simultaneously produces a therapeutic response and a humoral and cellular immune response in the body of the patient without resulting in deleterious side effects to the patient.

The present invention relates to a process for the production and recovery of a bio-surfactant extract from Bacillus sp. MCC0156. Further, the present invention relates to a thermostable bio-surfactant extract consisting of 1-Pentanonacontene and 3-hydroxy-16-methylheptadecanoic acid with excellent emulsification and oil displacement activity for applications in agriculture and oil recovery.

The present invention relates to a process for the production and recovery of a bio-surfactant extract from Bacillus sp. MCC0156. Further, the present invention relates to a thermostable bio-surfactant extract consisting of 1-Pentanonacontene and 3-hydroxy-16-methylheptadecanoic acid with excellent emulsification and oil displacement activity for applications in agriculture and oil recovery.

The present disclosure discloses a medium for primary isolation of Porphyromonas gingivalis, and a method for preparing the medium and use thereof. The medium for primary isolation of Porphyromonas gingivalis includes the following components in parts by weight: 10-20 parts of a mixed peptone, 5-10 parts of a yeast extract powder, 1-5 parts of sodium chloride, 10-15 parts of agar, 0.5-1.0 parts of glucose, 0.2-0.8 parts of sodium bicarbonate, 0.1-0.5 parts of an L-cysteine salt, 0.1-0.5 parts of soluble sodium pyrophosphate, 0.0001-0.0005 parts of hemin, 0.00001-0.00005 parts of vitamin K, 500-1000 parts of water and 5-10 parts of a sterile defiberized sheep blood. By adopting the medium for primary isolation of Porphyromonas gingivalis provided by examples of the present disclosure, a culture period of primary isolation of Porphyromonas gingivalis can be greatly shortened, and the primary isolation of Porphyromonas gingivalis can be quickly conducted.

The present disclosure discloses a medium for primary isolation of Porphyromonas gingivalis, and a method for preparing the medium and use thereof. The medium for primary isolation of Porphyromonas gingivalis includes the following components in parts by weight: 10-20 parts of a mixed peptone, 5-10 parts of a yeast extract powder, 1-5 parts of sodium chloride, 10-15 parts of agar, 0.5-1.0 parts of glucose, 0.2-0.8 parts of sodium bicarbonate, 0.1-0.5 parts of an L-cysteine salt, 0.1-0.5 parts of soluble sodium pyrophosphate, 0.0001-0.0005 parts of hemin, 0.00001-0.00005 parts of vitamin K, 500-1000 parts of water and 5-10 parts of a sterile defiberized sheep blood. By adopting the medium for primary isolation of Porphyromonas gingivalis provided by examples of the present disclosure, a culture period of primary isolation of Porphyromonas gingivalis can be greatly shortened, and the primary isolation of Porphyromonas gingivalis can be quickly conducted.

A cell picking device for sucking cells from a liquid sample in a sample container includes a sucking member to which a pipette tip is attachable, a driver that moves the sucking member and performs suction through the sucking member and the pipette tip, a work mode switcher that switches a work mode of the driver between a first mode and a second mode, and a controller that controls the driver.

A cell picking device for sucking cells from a liquid sample in a sample container includes a sucking member to which a pipette tip is attachable, a driver that moves the sucking member and performs suction through the sucking member and the pipette tip, a work mode switcher that switches a work mode of the driver between a first mode and a second mode, and a controller that controls the driver.