The invention pertains to a method for separating biomass from solid fermentation product wherein a slurry comprising biomass and solid fermentation product is provided to the top of a biomass separator unit and an aqueous medium is provided to the bottom of a biomass separator unit, while a product stream comprising solid fermentation product is withdrawn from the bottom of the biomass separator unit and a waste stream comprising biomass is withdrawn from the top of the biomass separator unit. It has been found that the method according to the invention makes it possible to provide a solid fermentation product which comprises very low amounts of remaining biomass, while product loss can be limited.

The invention pertains to a method for separating biomass from solid fermentation product wherein a slurry comprising biomass and solid fermentation product is provided to the top of a biomass separator unit and an aqueous medium is provided to the bottom of a biomass separator unit, while a product stream comprising solid fermentation product is withdrawn from the bottom of the biomass separator unit and a waste stream comprising biomass is withdrawn from the top of the biomass separator unit. It has been found that the method according to the invention makes it possible to provide a solid fermentation product which comprises very low amounts of remaining biomass, while product loss can be limited.

Provided is a technique capable of easily performing a microbial test with high accuracy. A microbial test kit of the present disclosure includes: a first syringe capable of collecting a specimen and having a first syringe needle; a second syringe containing an ATP extraction reagent and having a second syringe needle; a sealed reaction container having a first opening, a first sealing member fitted to the first opening, a nozzle, a nozzle cap covering the nozzle, and a filter disposed so as to partition the first opening and the nozzle; a waste liquid container including a second opening and a second sealing member fitted to the second opening, and sealed under reduced pressure; and a luminescence measurement container that includes a third opening and a third sealing member fitted to the third opening, stores a luminescent reagent that emits light in presence of ATP, and is sealed under reduced pressure.

Provided is a technique capable of easily performing a microbial test with high accuracy. A microbial test kit of the present disclosure includes: a first syringe capable of collecting a specimen and having a first syringe needle; a second syringe containing an ATP extraction reagent and having a second syringe needle; a sealed reaction container having a first opening, a first sealing member fitted to the first opening, a nozzle, a nozzle cap covering the nozzle, and a filter disposed so as to partition the first opening and the nozzle; a waste liquid container including a second opening and a second sealing member fitted to the second opening, and sealed under reduced pressure; and a luminescence measurement container that includes a third opening and a third sealing member fitted to the third opening, stores a luminescent reagent that emits light in presence of ATP, and is sealed under reduced pressure.

A cell manipulation method is provided including culturing cells in a liquid; disposing a flow path through which a gas is able to be introduced in the liquid; forming an air bubble at an end portion of the flow path; and attaching the cells to the air bubble.

The present invention discloses a bacterium and an obtaining method and application thereof. The bacterium has a property of coproducing 1,3-propanediol and D-lactic acid. Further, the bacterium is Klebsiella oxytoca, including Klebsiella oxytoca PDL-5 CCTCC M 2016185. The obtaining method of the bacterium may be to obtain the bacterium by directly screening wild bacteria that satisfy conditions from the environment or performing gene engineering modification to wild bacteria. The present invention has the advantages that the bacteria can coproduce 1,3-propanediol and D-lactic acid through fermentation, the molar conversion rate and the concentration of the two products are very high, the types of byproducts are few, the concentration is low, the product extraction process is simplified, the high-efficiency biological production of 1,3-propanediol and D-lactic acid can be realized, and the industrial application prospect is very great.

The present disclosure relates to a method of colony picking. The method includes the steps of mixing a bacterial suspension with an oil-based carrier liquid for generating a plurality of droplets comprising bacteria contained in the bacterial suspension and incubating the plurality of droplets for a predetermined period of time to allow growth of bacteria within the plurality of droplets. The method further includes the steps of screening each of the plurality of droplets that flows through one or more microfluidic channels of a microfluidic device to determine an opacity degree of each of the plurality of droplets, wherein the opacity degree is indicative of colony formation in the plurality of droplets, and sorting the plurality of droplets based on the opacity degree of each of the plurality of droplets.

The present disclosure relates to a method of colony picking. The method includes the steps of mixing a bacterial suspension with an oil-based carrier liquid for generating a plurality of droplets comprising bacteria contained in the bacterial suspension and incubating the plurality of droplets for a predetermined period of time to allow growth of bacteria within the plurality of droplets. The method further includes the steps of screening each of the plurality of droplets that flows through one or more microfluidic channels of a microfluidic device to determine an opacity degree of each of the plurality of droplets, wherein the opacity degree is indicative of colony formation in the plurality of droplets, and sorting the plurality of droplets based on the opacity degree of each of the plurality of droplets.

A cell picking device that sucks cells from a liquid sample in a sample container and accommodates the sucked cells in any of a plurality of wells of an accommodating plate, includes a suction discharger that sucks cells in the sample container and discharges the sucked cells into any of the plurality of wells, the receiver that receives selection of a well in which cells are to be accommodated out of the plurality of wells of the accommodating plate as a selection well, a driver that adjusts a relative positional relationship between the suction discharger and the plurality of wells of the accommodating plate, and a work controller that controls the driver such that the suction discharger discharges cells into the received selection well out of the plurality of wells of the accommodating plate.

A cell picking device that sucks cells from a liquid sample in a sample container and accommodates the sucked cells in any of a plurality of wells of an accommodating plate, includes a suction discharger that sucks cells in the sample container and discharges the sucked cells into any of the plurality of wells, the receiver that receives selection of a well in which cells are to be accommodated out of the plurality of wells of the accommodating plate as a selection well, a driver that adjusts a relative positional relationship between the suction discharger and the plurality of wells of the accommodating plate, and a work controller that controls the driver such that the suction discharger discharges cells into the received selection well out of the plurality of wells of the accommodating plate.