The present invention provides novel Lactococcus lactis lactic acid bacterium strains having improved texturing properties and methods of using the strains for producing food products.

Disclosed is a 3D porous medium and a method of manufacture. The 3D porous medium includes (i) a support structure of transparent hydrogel particles or emulsion droplets, (ii) bacterial nutrient in open volumes between the transparent hydrogel particles, as well as within micropores in the transparent hydrogel particles, and (iii) bacterial cells within the open volumes in the support structure.

Disclosed is a 3D porous medium and a method of manufacture. The 3D porous medium includes (i) a support structure of transparent hydrogel particles or emulsion droplets, (ii) bacterial nutrient in open volumes between the transparent hydrogel particles, as well as within micropores in the transparent hydrogel particles, and (iii) bacterial cells within the open volumes in the support structure.

Methods to increase viability and revivability of bacteria compared to their wild type counterparts, are disclosed herein. New strains of an anerobic bacteria is also disclosed obtained by the methods described herein. Further, disclosed are compositions comprising these bacteria as well as methods of treatment including the use of the bacteria described.

Crystalline gastroprotected granular bacteria, such as bacterial strains in granular form coated with a lipid coating matrix, preferably in a reduced amount, said lipid coating matrix having a crystalline form and related compositions and process of preparation are described.

Crystalline gastroprotected granular bacteria, such as bacterial strains in granular form coated with a lipid coating matrix, preferably in a reduced amount, said lipid coating matrix having a crystalline form and related compositions and process of preparation are described.

The disclosure provides methods and compositions for the preservation of bacteria. Aspects of the present disclosure provide methods of preparing a preserved bacterial composition comprising flash freezing a bacterial composition and lyophilizing the flash frozen bacterial composition to produce a preserved bacterial composition. In some embodiments, the bacterial composition comprises one or more bacterial strains. In some embodiments, the one or more bacterial strains comprise one or more anaerobic bacterial strains. In some embodiments, the anaerobic bacterial strains are strict anaerobic bacteria.

The disclosure provides methods and compositions for the preservation of bacteria. Aspects of the present disclosure provide methods of preparing a preserved bacterial composition comprising flash freezing a bacterial composition and lyophilizing the flash frozen bacterial composition to produce a preserved bacterial composition. In some embodiments, the bacterial composition comprises one or more bacterial strains. In some embodiments, the one or more bacterial strains comprise one or more anaerobic bacterial strains. In some embodiments, the anaerobic bacterial strains are strict anaerobic bacteria.

The present disclosure discloses a method for determining optimal preservation temperature of the anaerobic ammonia oxidation biofilm in wastewater treatment, and belongs to the technical field of environmental engineering. The method of the present disclosure characterizes the ratio of living cells, early apoptotic cells, late apoptotic cells and dead cells in the anaerobic ammonia oxidation biofilm by flow cytometry, and the optimum storage temperature can be measured within a few hours. The method of the present disclosure performs correlation analysis on the characteristic indexes of the anaerobic ammonia oxidation biofilm activity recovery process to verify the reliability of the data. By using the method of the present disclosure, the step of recovering the biofilm activity can be omitted, the removal rates of ammonia nitrogen and total nitrogen were over 90% and 85%, respectively.

The present invention relates to polymeric particles comprising a biodegradable polymer, and at least one microorganism in a total concentration of at least 10.sup.8 CFU/g dry weight that is stable for at least 35 weeks at 30° C. and optionally additional carriers and additives as well as to methods for producing polymeric particles and use thereof.