C12N1/06

Arrayed lyser and homogenizer systems with multiple agitator devices

Systems and methods for the efficient agitation of tissue samples. A device may include a plurality of chambers that each receives samples therein. The plurality of chambers may be uniformly spaced with respect to a least one dimension, to form a one dimensional or two dimensional array. Each of the chambers may include an opening and an agitator device in fluid contact with the sample disposed within the chamber. The agitator devices may include a micromotor which provides rotational motion to a shaft and an impeller fixed to the shaft such that the impeller and the shaft rotate together upon provision of the rotational motion by the micromotor. The system may include an electrical energy source electrically coupled to the plurality of micromotors to rotate the impellers sufficient to agitate the sample as required for a particular activity (e.g., homogenization, lysis).

Yeast proteins

The present invention relates to a method for obtaining yeast proteins comprising the following steps: a) providing a yeast cream; b) exposing this yeast cream to a thermal plasmolysis at a temperature between 70 and 95° C. for a period between 30 seconds and 4 hours, preferably between 1 minute and 3 hours, more preferably between 40 minutes and 2 hours; b′) separating the insoluble fraction and the soluble fraction; c) subjecting the insoluble fraction to the activity of at least one ribonuclease and a glucanase, sequentially or simultaneously, at a temperature between 40 and 65° C., preferably 60° C., for a period between 8 and 24 hours, preferably 18 hours; d) separating the insoluble fraction from the soluble fraction; wherein the insoluble fraction collected in step d) has no taste, having a nucleotide content less than 3% and a true protein content of at least 72%. Step b′) is optional. In this case, the entirety of the composition obtained after thermal plasmolysis of the yeast cream is subjected to enzymatic activity.

System, apparatus and method for material preparation and/or handling

Oscillating angularly rotating a container containing a material may cause the material to be separate. Denser or heavier material may unexpectedly tend to collected relatively close to the axis of rotation, while less dense or light material may tend to collect relatively away from the axis of rotation. Oscillation along an arcuate path provides high lysing efficiency. Alternatively, a micromotor may drive an impeller removably received in a container. Lysing may be implemented in batch mode, flow-through stop or semi-batch mode, or flow-through continuous mode. Lysing particulate material may exceed material to be lysed or lysed material and/or air may be essentially eliminated from a chamber to increase lysing efficiency.

Processes for obtaining colors from algal biomasses
11473114 · 2022-10-18 · ·

The present invention discloses processes for obtaining a color from an algal biomass. The process includes subjecting an algal biomass to cavitation, thus at least partially disrupting cells of the algal biomass and extracting color from the disrupted, algal biomass.

Processes for obtaining colors from algal biomasses
11473114 · 2022-10-18 · ·

The present invention discloses processes for obtaining a color from an algal biomass. The process includes subjecting an algal biomass to cavitation, thus at least partially disrupting cells of the algal biomass and extracting color from the disrupted, algal biomass.

System for obtaining protein-rich nutrient supplements from bacterial fermentation process

Protein-rich nutrient supplements and animal feed supplements derived from an anaerobic bacterial process are generated through a myriad of cell rupturing and protein fractionation/purification processes. Bacterial fermentation systems and methods of obtaining one or more protein-containing portions from a fermentation process using carbon monoxide-containing gaseous substrates are provided. The invention further provides compositions of protein-rich nutrient supplements with useful applications for intake by a variety of different animals and humans.

System for obtaining protein-rich nutrient supplements from bacterial fermentation process

Protein-rich nutrient supplements and animal feed supplements derived from an anaerobic bacterial process are generated through a myriad of cell rupturing and protein fractionation/purification processes. Bacterial fermentation systems and methods of obtaining one or more protein-containing portions from a fermentation process using carbon monoxide-containing gaseous substrates are provided. The invention further provides compositions of protein-rich nutrient supplements with useful applications for intake by a variety of different animals and humans.

TRANSPORT MEDIA FOR CLINICAL SPECIMEN COLLECTION AND MOLECULAR DIAGNOSTIC APPLICATIONS AND METHODS OF PRODUCTION AND USE THEREOF

A transport medium is disclosed that can be utilized for both sample collection and molecular diagnostic applications. The transport medium can be utilized with multiple types of biological samples and maintains the stability of nucleic acid present in the biological samples so that one or more nucleic acid assay target(s) present in the biological sample is not substantially degraded during storage and shipping. Also disclosed are kits containing the transport medium, mixtures that include a biological sample disposed in the transport medium, and methods of producing and using the medium.

Method for Producing Biomass from a Microalgae

A method for producing biomass from a microalgae includes culturing the microalgae in an effluent diluted in seawater. A method for bioremediating an effluent includes culturing a microalgae in the effluent diluted in seawater. The microalgae is at least one of a strain of the genus Nodularia, a strain of the genus Chrysoreinhardia, a strain of the genus Halochlorella, or combinations thereof. At the beginning of culturing, the diluted effluent exhibits concentrations of total nitrogen (N) in the range of 30-150 mg/l and concentrations of total phosphorus (P) in the range of 1-15 mg/l. The N/P quotient is in the range of 5-40.

METHOD FOR RECOVERING PHAS FROM A BIOMASS

A method for recovering polyhydroxyalkanoates from a biomass is disclosed. According to the method, polynucleotide chains are cleaved by addition of an endonuclease. A lysing agent is used to disrupt cell walls of the microorganism cells and release the intracellular polyhydroxyalkanoates from the cells. Proteins are also degraded by addition of a peptidase. The polyhydroxyalkanoates are then separated from cellular debris of the cells. According to the present disclosure, this method is carried out without the use of organic solvents in the cleaving, lysing, and degrading steps.