The invention discloses a construction method of genetic engineering fungi of filamentous fungi. Through the genetic engineering method, the filamentous fungi overexpress the positive regulation genes of ethanol synthesis, and/or down regulate the negative regulation genes of endogenous ethanol synthesis to obtain genetic engineering strains. Or overexpression of acetaldehyde dehydrogenase and ethanol dehydrogenase containing mitochondrial localization signal sequence, or overexpression of pyruvate decarboxylase and ethanol dehydrogenase containing mitochondrial localization signal sequence, or overexpression of acetaldehyde dehydrogenase, ethanol dehydrogenase and pyruvate decarboxylase containing mitochondrial localization signal sequence in filamentous fungal cells. Compared with the original strain, the ethanol synthesis ability of the obtained genetically engineered strains are improved.

The invention discloses a construction method of genetic engineering fungi of filamentous fungi. Through the genetic engineering method, the filamentous fungi overexpress the positive regulation genes of ethanol synthesis, and/or down regulate the negative regulation genes of endogenous ethanol synthesis to obtain genetic engineering strains. Or overexpression of acetaldehyde dehydrogenase and ethanol dehydrogenase containing mitochondrial localization signal sequence, or overexpression of pyruvate decarboxylase and ethanol dehydrogenase containing mitochondrial localization signal sequence, or overexpression of acetaldehyde dehydrogenase, ethanol dehydrogenase and pyruvate decarboxylase containing mitochondrial localization signal sequence in filamentous fungal cells. Compared with the original strain, the ethanol synthesis ability of the obtained genetically engineered strains are improved.

The present invention discloses an endophytic fungal strain Magnaporthaceae sp. M-B927 and an application thereof, and belongs to the technical filed of microbial applications. The deposit number of the endophytic fungal strain M-B927 is CCTCC M 2021503, and the scientific name thereof is Magnaporthaceae sp. The endophytic fungal strain M-B927 can enhance the resistance of rice against seedling leaf blast with a control efficiency of 73.06% and a disease index reduced by 52.22. Popularization and application of the biological control efficiency of the endophytic fungus M-B927 against seedling leaf blast in rice has great potential in the field of agriculture.

The present invention discloses an endophytic fungal strain Pseudophialophora sp. P-B313 and an application thereof, and belongs to the technical filed of microbial applications. The deposit number of the endophytic fungal strain P-B313 is CCTCC M 2021504, and the scientific name thereof is Pseudophialophora sp. The endophytic fungal strain P-B313 can enhance the resistance of rice against seedling leaf blast with a control efficiency of 87.56% and a disease index reduced by 62.59. The biological control efficiency of the endophytic fungus P-B313 against seedling leaf blast in rice has great value by promotion and applications thereof in the field of agriculture.

Unique carbon dioxide or lignocellulosic substrate is prepared and used to produce biosurfactants, based on different types of microorganism fermenting strains, using carbon dioxide or lignocellulose-based raw materials as the primary feedstock, subsequently utilizing a fermentation process to synthesize different structures of biosurfactants. This is a two-phase reaction where phase-one creates the feedstock for the phase-two reactions. The fermentation broth resulting from the phase-two reaction is the crude biosurfactant; it uses glycolipid or lipopeptide biosurfactant as the main component. The broth is then refined by filtration, then concentrated, and further purified to obtain the pure biosurfactant material. The biosurfactant of the present disclosure can be applied to industries such as petroleum, food or agriculture, daily chemicals, industrial chemicals, environmental protection, and medicine.

A Geomyces mutant strain and an application thereof, relating to the technical field of the screening and application of functional microorganisms; the mutant strain is obtained through a mutagenic method, capable of greatly improving the yield of red pigment; the mutant strain has been preserved in the China Center for Type Culture Collection of Wuhan University on Jan. 24, 2019, and the preservation number is CCTCC NO: M2019086; the mutant strain can be widely used in the field of natural pigment production, can reduce the production cost of red pigment, and has a bright application prospect.

A Geomyces mutant strain and an application thereof, relating to the technical field of the screening and application of functional microorganisms; the mutant strain is obtained through a mutagenic method, capable of greatly improving the yield of red pigment; the mutant strain has been preserved in the China Center for Type Culture Collection of Wuhan University on Jan. 24, 2019, and the preservation number is CCTCC NO: M2019086; the mutant strain can be widely used in the field of natural pigment production, can reduce the production cost of red pigment, and has a bright application prospect.

This document relates to methods for degrading Low Density Polyethylene (LDPE) and remediating leachate. This document provides methods involved in contacting pretreated LDPE or plastic waste comprising LDPE with at least one white-rot fungus, achieving near 100% LDPE degradation.

This document relates to methods for degrading Low Density Polyethylene (LDPE) and remediating leachate. This document provides methods involved in contacting pretreated LDPE or plastic waste comprising LDPE with at least one white-rot fungus, achieving near 100% LDPE degradation.

In preferred embodiments, the subject invention provides two-vessel fermentation systems for producing microbe-based products comprising fungal mycelia and/or spores, and/or bacterial endospores, wherein the systems comprise both a submerged fermentation vessel and a solid state fermentation (SSF) vessel. Advantageously use of the two phases improves the efficiency of producing microorganisms by catering to the different requirements for biomass and/or vegetative cell accumulation as well as the requirements for mycelial growth and/or sporulation.