The present invention discloses a method for preserving a probiotic composition, including: providing a bacterial cell suspension, which is one or more bacterial cell suspensions of a bacterium or Saccharomyces boulardii; mixing the bacterial cell suspension with a sodium alginate solution or an alginic acid solution; and adding the mixture to a calcium ion solution until the mixture is immobilized in a shape. The technology of the present invention has the effects of long-term preservation at room temperature and resistance to high temperature, and can be applied to ordinary bacterial strains, without being limited to a small number of bacterial species able to form endospore, and without requiring the strains to be frozen for preservation. The method of the present invention can be applied to the preparation of aquatic feeds, animal feeds, or probiotics that human beings need.

A method for manufacturing a fermentation product by culturing a microorganism, the method containing steps (A) to (D): (A) culturing the microorganism with a first culture medium; (B) passing a culture solution containing cultured bacterial cells, raw materials for culture, and the fermentation product through an adsorption tower packed with an adsorbent capable of adsorbing the fermentation product from the culture solution, and then collecting an effluent containing the bacterial cells and the raw materials for culture flowing out from the adsorption tower, wherein a relationship among a size (short diameter) d of the bacterial cell, a pore size D1 of the adsorbent, and a minimum void size D2 between adsorbent particles, D1<d<D2 is satisfied; (C) eluting the fermentation product from the absorbent; and (D) culturing the microorganism with a second culture medium using collected effluent containing the bacterial cells and the raw materials for culture.

The present invention discloses a method for preparing bread by fermentation with compounded sourdough, and belongs to the technical field of food. The method compounds saccharomycetes and lactic acid bacteria to prepare the compounded sourdough. Compared with spontaneous sourdough, the fermentation performance is similar, the quality is stable, the culture period is shortened, and the compounded sourdough can be used to replace the spontaneous sourdough to prepare bread by fermentation. Moreover, the nutritional value of the compounded sourdough bread is much higher than that of the commercial yeast bread using only a single type of yeast, both in terms of the total content of free amino acids and the content of essential amino acids. The compounded sourdough provided by the present invention has extremely high industrial application value.

The present invention discloses a method for preparing bread by fermentation with compounded sourdough, and belongs to the technical field of food. The method compounds saccharomycetes and lactic acid bacteria to prepare the compounded sourdough. Compared with spontaneous sourdough, the fermentation performance is similar, the quality is stable, the culture period is shortened, and the compounded sourdough can be used to replace the spontaneous sourdough to prepare bread by fermentation. Moreover, the nutritional value of the compounded sourdough bread is much higher than that of the commercial yeast bread using only a single type of yeast, both in terms of the total content of free amino acids and the content of essential amino acids. The compounded sourdough provided by the present invention has extremely high industrial application value.

The disclosure discloses recombinant Bacillus subtilis for synthesizing guanosine diphosphate fucose and a construction method and application thereof. The recombinant Bacillus subtilis is obtained by intensively expressing guanylate kinase and nucleotide diphosphokinase genes and expressing exogenous fucokinase and phosphate guanylyltransferase genes in a genome of Bacillus subtilis 168. According to the disclosure, a bacterial strain for synthesizing the guanosine diphosphate fucose is obtained by reconstructing the Bacillus subtilis 168, with a volume of intracellular accumulation up to 196.15 g/L. According to the disclosure, by intensively expressing the guanylate kinase and nucleotide diphosphokinase genes, and enhancing the supply of intracellular GDP-L-fucose composition cofactors, the synthesis of the guanosine diphosphate fucose is promoted. The construction method for the recombinant Bacillus subtilis of the disclosure is simple and convenient to use, thus having good application prospects.

The invention pertains to novel methods to increase the tolerance of microbial host cells to toxic substance, for example terpenes and alcohols and other membrane disrupting substances, as well as modified organism with such an increased tolerance a compared to the unmodified organism.

The invention pertains to novel methods to increase the tolerance of microbial host cells to toxic substance, for example terpenes and alcohols and other membrane disrupting substances, as well as modified organism with such an increased tolerance a compared to the unmodified organism.

A method for producing PHA polymer includes using bacteria in which the bacteria are grown under heterotrophic conditions using an organic substance as carbon source and exponential growth conditions. The bacteria are then cultivated under autotrophic conditions under an atmosphere of H.sub.2, CO.sub.2 and O.sub.2, wherein the O.sub.2 content is less than 10% (v/v) and the pressure is more than 1 barg.

A method for producing PHA polymer includes using bacteria in which the bacteria are grown under heterotrophic conditions using an organic substance as carbon source and exponential growth conditions. The bacteria are then cultivated under autotrophic conditions under an atmosphere of H.sub.2, CO.sub.2 and O.sub.2, wherein the O.sub.2 content is less than 10% (v/v) and the pressure is more than 1 barg.

The present application refers to oral compositions comprising an effective amount of a Pediococcus acidilactici strain for use in regulating blood glucose levels, as well as for the prevention and/or treatment of hyperglycemia and/or a hyperglycemia-related condition. The application also refers to Pediococcus acidilactici CECT 9879, Pediococcus acidilactici CECT 9889 and Pediococcus acidilactici CECT 9967, and to these strains for use as probiotics, medicaments and for regulating blood glucose levels, as well as for the prevention and/or treatment of hyperglycemia and/or a hyperglycemia-related condition.