Disclosed is a high-throughput transcriptional assay format in Actinomycete bacteria, and Streptomyces spp. in particular, that leverages eGFP, inserted both at a neutral site and inside the biosynthetic cluster of interest, as a read-out for secondary metabolite synthesis. Using this approach, a silent gene cluster in Streptomyces albus J1074 was induced. The cytotoxins etoposide and ivermectin were revealed as potent inducers, allowing the isolation and structural characterization of nearly 20 novel small molecule products of the chosen cluster. One of these molecules is a novel antifungal, while several others inhibit a cysteine protease implicated in cancer. Studies addressing the mechanism of induction by the two elicitors led to the identification of a pathway-specific transcriptional repressor that silences the gene cluster under normal growth conditions. The successful implementation of this approach will allow future discovery of cryptic metabolites with useful bioactivities from Actinomycete bacteria.

Provided herein are devices and methods that enable co-incubation of microorganisms. Also provided are methods of making such devices for co-incubation of microorganisms, and various applications of such devices.

Provided herein are devices and methods that enable co-incubation of microorganisms. Also provided are methods of making such devices for co-incubation of microorganisms, and various applications of such devices.

A method of forming an ethanol fermentation enhancement mixture is provided and involves: hydrating a dried yeast with at least 0.1% Baclyte or a banana extract by volume, and a yeast growth media to produce a pre-fermentation mixture; and maintaining the pre-fermentation mixture at a temperature between 20° C. and 40° C. for between 30 minutes and 8 hours. Alternatively, the method involves: providing a solution of hydrated activated yeast; supplementing the solution of hydrated activated yeast with 0.1% to 25% BacLyte or banana extract by volume; and maintaining the solution of hydrated activated yeast at a temperature between 20° C. and 40° C. for between 30 minutes and 8 hours. A fermentation method involves preparing an ethanol enhancement mixture; adding the mixture to a bulk fermentation mixture containing a sugar source; and maintaining the bulk fermentation mixture at temperature of between 2° C. and 40° C. to allow fermentation of the sugar source to ethanol.

A method of forming an ethanol fermentation enhancement mixture is provided and involves: hydrating a dried yeast with at least 0.1% Baclyte or a banana extract by volume, and a yeast growth media to produce a pre-fermentation mixture; and maintaining the pre-fermentation mixture at a temperature between 20° C. and 40° C. for between 30 minutes and 8 hours. Alternatively, the method involves: providing a solution of hydrated activated yeast; supplementing the solution of hydrated activated yeast with 0.1% to 25% BacLyte or banana extract by volume; and maintaining the solution of hydrated activated yeast at a temperature between 20° C. and 40° C. for between 30 minutes and 8 hours. A fermentation method involves preparing an ethanol enhancement mixture; adding the mixture to a bulk fermentation mixture containing a sugar source; and maintaining the bulk fermentation mixture at temperature of between 2° C. and 40° C. to allow fermentation of the sugar source to ethanol.

A method for producing a polyhydroxyalkanoate that includes the following steps: (a) a step for culturing halobacteria with a culture medium containing sea water; and (b) a step for obtaining a polyhydroxyalkanoate as product of the culture.

The present invention relates to a method for producing a recombinant protein in yeast cells, wherein the cells are subjected to a temperature shift at a specific timepoint of the cell culture. It also relates to a method for producing a recombinant protein in yeast cells by culturing said yeast cells in a medium having a high concentration of potassium ions compared to the concentration of sulfate and/or phosphate ions.

The present invention relates to a method for producing a recombinant protein in yeast cells, wherein the cells are subjected to a temperature shift at a specific timepoint of the cell culture. It also relates to a method for producing a recombinant protein in yeast cells by culturing said yeast cells in a medium having a high concentration of potassium ions compared to the concentration of sulfate and/or phosphate ions.

Provided are a lactic acid bacteria culture medium comprising red ginseng-derived dietary fiber; and a method for culturing lactic acid bacteria by using same, wherein red ginseng-derived dietary fiber is used in a lactic acid bacteria culture medium so that the number of viable lactic acid bacteria can be increased and the culturing thereof can be improved, and the method uses the culture medium so as to improve culturing under the same conditions, and thus is advantageous in terms of cost.

An application of geraniol in preparation of formulation for promoting synthesis of Pseudomonas aeruginosa 3OC.sub.12-HSL signal molecules is provided. It was found that geraniol slightly inhibits growth of Pseudomonas aeruginosa PAO1 strain, but can significantly promote synthesis of the 3OC.sub.12-HSL signal molecules of the bacterium, and thus can be applied to preparation of formulation for promoting synthesis of the Pseudomonas aeruginosa 3OC.sub.12-HSL signal molecules.