The present invention relates to a lysosomal protein composition comprising a plurality of lysosomal proteins that are potentially diversely glycosylated according to a glycosylation pattern, wherein said glycosylation pattern has at least 45% paucimannosidic N-glycans; a method of manufacturing the lysosomal protein composition in a bryophyte plant or cell, and medical and non-medical uses of the lysosomal protein composition. E.g. the lysosomal protein can be α-Galactosidase for the treatment of Fabry Disease or β-Glucoceramidase for the treatment of Gaucher's Disease. The unique glycosylation results in improved therapeutic efficacy—surprisingly even without mannose-6-phosphate that is common for CHO cell produced lysosomal proteins.

The present invention relates to a Lactuca sativa seed designated 79-141 RZ. The present invention also relates to a Lactuca sativa plant produced by growing the 79-141 RZ seed. The invention further relates to methods for producing the lettuce cultivar, represented by lettuce variety 79-141 RZ.

The present invention relates to a Lactuca sativa seed designated 79-141 RZ. The present invention also relates to a Lactuca sativa plant produced by growing the 79-141 RZ seed. The invention further relates to methods for producing the lettuce cultivar, represented by lettuce variety 79-141 RZ.

The process described herein may provide the benefit of selectively generating plant-based materials with tunable cellular compositions and material properties in controlled forms without necessarily requiring whole-plant cultivation or harvest. An example process may include extracting and maintaining live plant cells via leaf maceration and liquid culturing, transferring cells from the liquid culture to a gel medium, integrating the cells into a hydrogel scaffold, and shaping the scaffold. This process, using the disclosed tissue engineering-style approach, may further allow for localized and high-density biomass production, eliminate energy intensive harvest and hauling, reduce processing, and inherently foster climate resilience.

The process described herein may provide the benefit of selectively generating plant-based materials with tunable cellular compositions and material properties in controlled forms without necessarily requiring whole-plant cultivation or harvest. An example process may include extracting and maintaining live plant cells via leaf maceration and liquid culturing, transferring cells from the liquid culture to a gel medium, integrating the cells into a hydrogel scaffold, and shaping the scaffold. This process, using the disclosed tissue engineering-style approach, may further allow for localized and high-density biomass production, eliminate energy intensive harvest and hauling, reduce processing, and inherently foster climate resilience.

Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.

Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.

A method of producing plant-derived exosomes from plant tissue culture based cell suspension cultures is provided. The method includes: obtaining the plant tissue culture based cell suspension culture; mixing the the plant tissue culture based cell suspension culture with an isolation solution; centrifuging to obtain a supernatant and an infranatant; and obtaining the plant-derived exosomes from the infranatant. The objective of the present invention is to produce homogenous plant exosomes with high volume and purity by making use of the advantages of the plant suspension culture to be used for purposes such as therapeutics and drug carriers.

A method of producing plant-derived exosomes from plant tissue culture based cell suspension cultures is provided. The method includes: obtaining the plant tissue culture based cell suspension culture; mixing the the plant tissue culture based cell suspension culture with an isolation solution; centrifuging to obtain a supernatant and an infranatant; and obtaining the plant-derived exosomes from the infranatant. The objective of the present invention is to produce homogenous plant exosomes with high volume and purity by making use of the advantages of the plant suspension culture to be used for purposes such as therapeutics and drug carriers.

A device for directionally placing a plant propagule (2) inside a hollow member (3), as exemplarily illustrated in FIGS. 4-7, comprising a platform element (20) arranged such that a foldable member (1) can be placed thereon; an actuating dispensing arrangement (90a, 90b, 100) for placing a foldable member (1) on the platform element (20); an arrangement (40)/(40a)for placing a plant propagule (2) on a foldable member (1) placed on the platform element (20); optionally, means (150) for identifying an imaginary line (9) on the foldable member (1) stretching through the root forming end (7) to the shoot forming end (8) of the propagule (2) being directionally placed during operation; an actuating folding arrangement (30, 80)/(120/80) for folding the foldable member (1) along said imaginary line (9) to form a folded foldable member (1a); an actuating dispensing arrangement (180) for providing a hollow member (3) having a first open end (4); and an actuating placing arrangement (30, 80)/(120/80) for placing said folded foldable member (1a) into the hollow member (3) through the first open end (4). Related methods for handling plant propagules, in particular somatic plant embryos.