The present invention provides a construction method for ecologically protecting an expansive soil slope by combining phosphogypsum with microbial mineralization. The method includes: (1) placing Bacillus pasteurii in a culture medium to prepare a microbial solution, and mixing urea, calcium chloride and water to prepare a cementing solution; (2) preparing a mixture with phosphogypsum fly ash and soil; mixing the mixture, the microbial solution and water well, and adding the cementing solution and water to prepare an improving mixture slurry; and (3) spraying the improving mixture slurry to a face of the slope by wet spraying, and covering with a non-woven fabric by tying and fixing.

Described are means, methods and compositions of matter useful for enhancing quality of organ transplants by induction of postmortem regeneration. The disclosure provides administration of regenerative cells and/or factors in a brain dead recipient whose body is maintained in a viable state by life supporting machinery.

The invention provides a method and related compositions for preparing an effective probiotic bacteria formulation that can be stored, transported and used for protecting a variety of marine organisms against infection by potential pathogens.

The present invention provides methods or kits with inflammatory cytokines to pretreat 1-ISCs to augment their immune modulatory effect, in prevention and treatment of various diseases such as multiple sclerosis, arthritis, lupus, sepsis, hepatitis, cirrhosis, Parkinson's disease, chronic infections, and GvHD. The present invention relates to novel methods for enhancing the immunosuppressive or the immune stimulatory activities of mesenchymal stem cells (JvfSCs).

The present disclosure relates to a composition for promoting the growth of lactic acid bacteria comprising growth factors, and more particularly, to a composition for promoting the growth of lactic acid bacteria comprising growth factors, the composition being capable of efficiently promoting the growth of various lactic acid bacteria strains.

Aspects of the invention are directed to mineral-based nanoparticles comprising silicate nanoparticles that induce human mesenchymal stem cells (hMSCs) into a cartilage-lineage through the upregulation of cartilage-specific genes resulting in the transformation of the cell phenotype into that of a chondrocyte, i.e., a cartilage producing cell. The silicate nanoparticles are synthesized through a process where the precipitate of sodium silicate is mixed with one or more elements and compounds and milled into nanoparticles.

An apparatus and method to maintain pH within a range conducive for cell growth in a bicarbonate-containing cell culture system without the addition of base. The method relies on the gas transfer characteristics of the bioreactor system to modulate the CO.sub.2 transfer to and from the cell culture such that the pH of the cell culture can be maintained within a desired range.

The present invention is directed generally to dry cell culture media or feeds in pellet formats which can be reconstituted into liquid media for culturing cells in vitro. Each pellet composition may comprise the same or a different composition; for example, different vitamins, amino acids, buffers, trace salts, pH, iron chelators, etc. The invention also relates to methods of making dry cell culture media by altering ratios of different pellet compositions, or, methods of making modular dry cell culture media, or customizing media formulations for growing a cell type using pellets. The media pellets may be easier to handle either before reconstitution, during shipping and handling; and/or during reconstitution. Media pellets may be used in any container like bags including sterile, single use bags for preparing media formulations. The invention also relates to kits and culture systems using media pellets.

Provided is a method of producing an oil containing polyunsaturated fatty acids by using Schizochytrium sp. The method comprises: performing fermentation using Schizochytrium sp.; resuspending the resultant cells after fermentation in water, adding cellulase and neutral protease for enzymolysis; mixing the enzymatic hydrolyzate with n-hexane, shaking, extracting, centrifuging, and collecting the n-hexane phase; concentrating the n-hexane phase under reduced pressure to remove the n-hexane, and drying to obtain an oil; performing a first crystallization and a second crystallization, and then performing cold filtration to obtain a liquid oil after the second crystallization; and subjecting the liquid oil to deacidification and decolorization. By regulating fermentation conditions and conducting concentration processing on polyunsaturated fatty acids, the content of eicosapentaenoic acid in the Schizochytrium sp. is increased to more than 12%, and the obtained oil is rich in docosahexaenoic acid, docosapentaenoic acid and eicosapentaenoic acid.

Provided is a recombinant yeast expressing germacrene A synthetase or a fusion protein thereof, wherein the fusion protein is germacrene A synthetase and farnesyl pyrophosphate synthase. The recombinant yeast improves the yield of germacrene A, and is suitable for the industrialized production of -elemene and/or germacrene A.