Provided is a method for manufacturing cell-containing blocks having steps of: preparing an standardized size mold by 3D printing (three dimensional printing) using a biocompatible elastic material; injecting a thermosensitive colloid into the mold to form a thermosensitive mold; injecting a hydrogel containing cells in to the thermosensitive mold and curing the hydrogel containing cells to form the cell-containing blocks; separating the thermosensitive mold and the cell-containing blocks at a temperature higher than a solidifying point of the thermosensitive colloid. Also provided are method for assembling the cell-containing blocks in a target configuration by using an assembling mold defining the target configuration and made of a thermoreversible material.

Provided herein are methods and systems for bio-printing of three-dimensional organs and organoids. Also provided herein are bio-printed three-dimensional organs and organoids for use in the generation and/or the assessment of immunological products and/or immune responses. Also provided herein are methods and system for bio-printing three-dimensional matrices.

The disclosure relates to a chondrogenic cell sheet comprising at least two layers of confluent chondrogenically differentiated cells, wherein the cell sheet is prepared from mesenchymal stem cells (MSCs), and wherein the chondrogenically differentiated cells on the basal side of the cell sheet express adhesion molecules. The disclosure also relates to a method of preparing a chondrogenic cell sheet comprising: a) culturing MSCs on temperature responsive cultureware until confluent to form a cell sheet; b) detaching the cell sheet by temperature reduction and allowing the cell sheet to contract, forming a contracted cell sheet; c) contacting the contracted cell sheet with a culture surface; and d) treating the contracted cell sheet on the culture surface with chondrogenic medium and culturing to form a chondrogenic cell sheet. Methods of using the chondrogenic cell sheets to repair cartilage tissue or treat joint disease are also disclosed.

Provided is a cell sheet support that satisfactory adheres to cultured cell and has biodegradability. The cell sheet support contains a first polymer containing a structural unit derived from p-dioxanone. The cell sheet support further contains a second polymer containing at least one selected from the group consisting of polylactic acid, polyglycolic acid, polycaproic acid, and copolymers thereof. The content ratio of the first polymer to the total amount of the first polymer and the second polymer is not less than 50% by mass. The cell sheet support has a sheet shape having an average thickness of 10 ?m to 150 ?m.

Thin film coatings and methods of manufacture thereof are presented. Ultra-thin coatings of cross-linked high-methoxyl pectin polysaccharides were fabricated by spin-casting solutions of citrus pectin followed by cross-linking upon exposure to solutions of calcium chloride (CaCl.sub.2) in ethanol. By adjusting temperature, degree of cross-linking, and pH of the surroundings, the pectin coatings can be carefully tuned for a desired response.

A diseased site where an anterior segment tissue is partly or entirely damaged or deficient can be treated using a corneal epithelium forming cell sheet that will adhere well to the anterior segment tissue. To attain this objective, a corneal epithelium forming cell sheet is produced by a process comprising the steps of cultivating under specified conditions corneal epithelium forming cells on a cell culture support comprising a substrate having its surface covered with a temperature responsive polymer of which the hydrating force varies in a temperature range of 0 C.-80 C., optionally stratifying the layer of cultured cells, and thereafter, (1) adjusting the temperature of the culture solution to either above an upper critical dissolution temperature or below a lower critical dissolution temperature, (2) bringing the cultured corneal epithelium forming cells into close contact with a carrier, and (3) detaching the sheet together with the carrier under specified conditions.

The present invention relates to a method for selective cell attachment/detachment, cell patternization and cell harvesting by means of near infrared rays. More particularly, conducting polymers or metal oxides having exothermic characteristics upon irradiation of near infrared light is used as a cell culture scaffold, thus selectively attaching/detaching cells without an enzyme treatment. The scaffold has an effect of promoting proliferation or differentiation of stem cells, and therefore, can be used as a stem cell culture scaffold. The scaffold enables cell attachment/detachment without temporal or spatial restrictions, thus enabling cell patternization.

An infusible three-dimensional network of crosslinked acrylic-type polymer fibers, where the diameter of the fibers is between 0.1 and 1.5 ?m, the size of the interstices between the fibers is between 0.1 and 50 ?m.sup.2 and the stiffness of the network includes an elastic modulus between 0.01 and 10,000 kPa.

A tissue engineered periosteum for a patient comprising a sheet of stem cells. A method of repairing bone injury on a patient comprising implanting into the patient an engineered bone graph comprising a scaffold wrapped in a tissue engineered periosteum. A tissue engineered bone graph comprising a scaffold and a tissue engineered periosteum wrapped around an exterior of the scaffold.

A system for detecting rare cells in a fluid is disclosed. The system includes a substrate and a mixture disposed on the substrate and including a carrier and a thermo-responsive polymer for capture and release of the rare cells. Also disclosed is a method for detecting rare cells in a fluid using a system including a substrate and a mixture that is disposed on the substrate. The mixture includes a carrier and a thermo-responsive polymer. The method includes providing the system and introducing a sample of fluid containing the rare cells into the system such that the sample interacts with the carrier for capturing the rare cells.