The present invention relates to a method for converting at least one oligo- and/or polysaccharide into fructose comprising the steps of: a) adding to a composition comprising water, phosphate and at least one oligo- and/or polysaccharide at least four enzymes, and b) subsequently enzymatically converting the at least one oligo- and/or polysaccharide to fructose in the presence of the at least four enzymes, wherein in step a) at least one additional saccharide is added, whereby the at least one additional saccharide is selected from the group consisting of saccharides comprising 20 or less monosaccharide residues and/or combinations thereof; wherein in step a) the at least four enzymes, preferably at least five enzymes, are selected from the group consisting of transferases, phosphorylases, mutases, isomerases, hydrolases, phosphatases and combinations thereof; and wherein at least one enzyme in step a) is a phosphatase.

The present disclosure relates to a composition for tagatose production and a method for preparing tagatose using the same.

Culturing S. glucoliberatum PABB004 under conditions effective for the S. glucoliberatum PABB004 to secrete simple sugars into culture medium. In one or more embodiments, the conditions include a pH of 6.0 to 8.5. In some cases, the culture can include a second organism. A co-culture includes S. glucoliberatum PABB004 and a second organism, wherein the co-culture has a pH of 6.0 or greater. In one or more embodiments, the second organism is selected to produce a product of interest such as, for example, ethanol.

Culturing S. glucoliberatum PABB004 under conditions effective for the S. glucoliberatum PABB004 to secrete simple sugars into culture medium. In one or more embodiments, the conditions include a pH of 6.0 to 8.5. In some cases, the culture can include a second organism. A co-culture includes S. glucoliberatum PABB004 and a second organism, wherein the co-culture has a pH of 6.0 or greater. In one or more embodiments, the second organism is selected to produce a product of interest such as, for example, ethanol.

The present invention relates to isolated polypeptides having laccase activity and polynucleotides encoding the polypeptides and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

The present invention relates to isolated polypeptides having laccase activity and polynucleotides encoding the polypeptides and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Provided are certain glycosyl hydrolase family 3 (GH3) beta-xylosidases engineered to acquire beta-glucosidase activities. Provided also are compositions comprising such multi-functional GH3 enzymes and methods of use or industrial applications thereof.

Provided are certain glycosyl hydrolase family 3 (GH3) beta-xylosidases engineered to acquire beta-glucosidase activities. Provided also are compositions comprising such multi-functional GH3 enzymes and methods of use or industrial applications thereof.

A method of efficiently producing saccharides having glucose as the major component by inexpensively suppressing the non-productive adsorption of the enzyme to lignin is provided. The method of producing saccharides includes: a first step of preparing a water-soluble protein by adding at least any one of an animal protein and a vegetable protein to an aqueous sodium hydroxide solution or an aqueous calcium hydroxide solution to react with each other; a second step of adding the water-soluble protein to a slurry including a biomass; and a third step of producing saccharides having glucose as a major component by adding a degrading enzyme to the slurry for at least any one of a cellulose or a hemicellulose included in the biomass to be degraded by the degrading enzyme simultaneously with addition of the water-soluble protein to the slurry or after addition of the water-soluble protein.

A method of efficiently producing saccharides having glucose as the major component by inexpensively suppressing the non-productive adsorption of the enzyme to lignin is provided. The method of producing saccharides includes: a first step of preparing a water-soluble protein by adding at least any one of an animal protein and a vegetable protein to an aqueous sodium hydroxide solution or an aqueous calcium hydroxide solution to react with each other; a second step of adding the water-soluble protein to a slurry including a biomass; and a third step of producing saccharides having glucose as a major component by adding a degrading enzyme to the slurry for at least any one of a cellulose or a hemicellulose included in the biomass to be degraded by the degrading enzyme simultaneously with addition of the water-soluble protein to the slurry or after addition of the water-soluble protein.