This specification discloses process for obtaining maltodextrin having DE between 17 and 19.9 and the maltodextrins obtained from the process. The disclosed maltodextrins can be provided as a powder or in shelf stable liquid form. The disclose maltodextrins have a polysaccharide profile similar to those observed for prior art maltodextrins, but make maltodextrin solutions having a high solids content, but reduced viscosity compared to prior art maltodextrins, on equivalent solids-in-solution basis. The process combines adds an alpha-amylase and a pullulanase enzyme to a polysaccharide mixture during a saccharification step. The disclosed maltodextrins make solutions at 50° C. and greater than 65% on a solids dry solids basis having a viscosity between 5,000 and 12,000 cP and having a water activity of less than 0.80.

The disclosure relates to a preparation method of a amylodextrin and belongs to the technical field of starch chemical modification. According to the method, de-clustering and complexation effects of ultrasonic waves are used to achieve de-clustering of a starch chain and complexation of an amorphous region and an emulsifier, and then -amylase and pullulanase are used to achieve complex enzymolysis. Because the amorphous region and the emulsifier form a complex which is resistant to enzymolysis, the amorphous region is prevented from being destroyed. Finally, dextrins of different molecular weights are separated by a membrane separation method, so as to obtain a amylodextrin product with low polydispersity coefficient and narrow molecular weight distribution, and the starch comprehensive utilization efficiency is increased to 70% or above.

The disclosure relates to a preparation method of a amylodextrin and belongs to the technical field of starch chemical modification. According to the method, de-clustering and complexation effects of ultrasonic waves are used to achieve de-clustering of a starch chain and complexation of an amorphous region and an emulsifier, and then -amylase and pullulanase are used to achieve complex enzymolysis. Because the amorphous region and the emulsifier form a complex which is resistant to enzymolysis, the amorphous region is prevented from being destroyed. Finally, dextrins of different molecular weights are separated by a membrane separation method, so as to obtain a amylodextrin product with low polydispersity coefficient and narrow molecular weight distribution, and the starch comprehensive utilization efficiency is increased to 70% or above.

Embodiments of the present disclosure relate to materials and methods for preparing a clathrate-forming composition comprising a plurality of linear glucomonomer chains of about 15 to about 100 D-glucopyranosyl residues linked by -1,4 linkages, wherein the linear glucomonomer chains are a product of partial amylolysis of a modified starch substrate and wherein the product is flowable at temperatures within a range of 4-20 C. at about 20% w/v solids content. The present disclosure further describes methods of using the clathrate-forming compositions to form molecular dispersions or clathrates with hydrophobic guest molecules, kits for use in these methods, and molecular dispersions or clathrates obtained from the materials.

Embodiments of the present disclosure relate to materials and methods for preparing a clathrate-forming composition comprising a plurality of linear glucomonomer chains of about 15 to about 100 D-glucopyranosyl residues linked by -1,4 linkages, wherein the linear glucomonomer chains are a product of partial amylolysis of a modified starch substrate and wherein the product is flowable at temperatures within a range of 4-20 C. at about 20% w/v solids content. The present disclosure further describes methods of using the clathrate-forming compositions to form molecular dispersions or clathrates with hydrophobic guest molecules, kits for use in these methods, and molecular dispersions or clathrates obtained from the materials.

Processes for grinding corn, ground corn products, and processes for making ethanol from the ground corn products. In some examples, a process for making ethanol can include introducing a plurality of corn pieces into a mill. The process can also include milling the corn pieces in the mill to produce a ground corn product. Greater than 25 wt % of the ground corn product can have a particle size of greater than 105 m, as measured according to AOAC 965.22-1966. Greater than 80 wt % of the ground corn product can have a particle size of 425 m or less, as measured according to AOAC 965.22-1966. The process can also include processing the ground corn product to produce a fermentation mash that can include ethanol and separating at least a portion of the ethanol from the fermentation mash to produce a stillage.

Processes for grinding corn, ground corn products, and processes for making ethanol from the ground corn products. In some examples, a process for making ethanol can include introducing a plurality of corn pieces into a mill. The process can also include milling the corn pieces in the mill to produce a ground corn product. Greater than 25 wt % of the ground corn product can have a particle size of greater than 105 m, as measured according to AOAC 965.22-1966. Greater than 80 wt % of the ground corn product can have a particle size of 425 m or less, as measured according to AOAC 965.22-1966. The process can also include processing the ground corn product to produce a fermentation mash that can include ethanol and separating at least a portion of the ethanol from the fermentation mash to produce a stillage.

Green preparation methods of rice resistant starch are disclosed. In some embodiments, a green preparation method of the rice resistant starch is characterized in that, at a temperature lower than the gelatinization temperature, the rice starch is sequentially modified by enzymes using (-amylase, glucosidase, and pullulanase to obtain the modified starch. In other embodiments, a green preparation method of the rice resistant starch is characterized by using: rice starch as a substrate; and in turn using: (a) -amylase (BA, EC 3.2.1.2) from barley (Hordeum vulgare); (b) glucoside transferase (TG, EC 2.4.1.24) from Aspergillus niger; and (c) pullulanase (PUL, EC 3.2.1.41) from Pullulanibacillus konaensis below a gelatinization temperature to modify a chain structure of the rice starch, resulting in a number of short linear chains which are effectively arranged, aggregated, and recrystallized at 4 C. to form modified rice starch with high resistant starch content.

Green preparation methods of rice resistant starch are disclosed. In some embodiments, a green preparation method of the rice resistant starch is characterized in that, at a temperature lower than the gelatinization temperature, the rice starch is sequentially modified by enzymes using (-amylase, glucosidase, and pullulanase to obtain the modified starch. In other embodiments, a green preparation method of the rice resistant starch is characterized by using: rice starch as a substrate; and in turn using: (a) -amylase (BA, EC 3.2.1.2) from barley (Hordeum vulgare); (b) glucoside transferase (TG, EC 2.4.1.24) from Aspergillus niger; and (c) pullulanase (PUL, EC 3.2.1.41) from Pullulanibacillus konaensis below a gelatinization temperature to modify a chain structure of the rice starch, resulting in a number of short linear chains which are effectively arranged, aggregated, and recrystallized at 4 C. to form modified rice starch with high resistant starch content.

The present invention relates to a method for obtaining a high titer of enzyme mixture comprising cellulases, hemicellulases and -glucosidases in reutilization of waste water generated during hot water extraction of lignocellulosic biomass or biorefinery waste water using Penicillium funiculosum MRJ-16 mutant strain. The cellulose or lignocellulosic biomass used in the fermentation process is selected from the group consisting of rice straw, wheat straw, corn stover, cotton stalk or a combination thereof. The enzyme mixture obtained by the present process is used for the saccharification of acid pretreated lignocellulosic biomass.