Various recovery processes are provided for the complete recovery of low soluble steviol glycosides obtained in recombinant microorganisms. Soluble -glycosyl steviol glycosides were fully recovered in downstream processing and then converted to steviol glycosides by hydrolases. The obtained steviol glycosides were purified and used as sweeteners, sweetness enhancers, flavor enhancers, and flavor modifiers in foods, beverages, cosmetics and pharmaceuticals.

The present disclosure relates to polypeptides having alpha-glucosidase activity isolated, derived or derivable from Rasamsonia or engineered polypeptides having alpha-glucosidase activity isolated, derived or derivable from Rasamsonia homologs. The present disclosure also pertains to polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, host cells and mutant cells comprising the polynucleotides. The disclosure further pertains to compositions comprising such polypeptides, methods of producing the polypeptides and compositions, as well as methods for using such polypeptides and compositions for industrial applications.

This disclosure relates to methods of isolating CDs. The method includes contacting a CD production mixture containing CD, CD, CD, and CD production byproducts with a metal salt; and forming CD-MOF complexes containing at least a metal cation and a plurality of CD components.

The present invention relates to isolated polypeptides having glucoamylase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

With a method for digesting starch, an aqueous slurry of the starch is treated with steam in a cooking vessel and in this case exposed to shear forces, wherein the starch-containing slurry is heated to a temperature of between 85C and 110C in the cooking vessel by introducing steam, and the digestion step is implemented until the desired degree of digestion has been reached. Also described is a cooking vessel that can be used when the method for digesting starch is being carried out.

With a method for digesting starch, an aqueous slurry of the starch is treated with steam in a cooking vessel and in this case exposed to shear forces, wherein the starch-containing slurry is heated to a temperature of between 85C and 110C in the cooking vessel by introducing steam, and the digestion step is implemented until the desired degree of digestion has been reached. Also described is a cooking vessel that can be used when the method for digesting starch is being carried out.

An engineered pathway for carbon fixation without rubisco incorporates carbon dioxide into fructose-6-phosphate.

An engineered pathway for carbon fixation without rubisco incorporates carbon dioxide into fructose-6-phosphate.

With a method for digesting starch, an aqueous slurry of the starch is treated with steam in a cooking vessel and in this case exposed to shear forces, wherein the starch-containing slurry is heated to a temperature of between 85 C. and 110 C. in the cooking vessel by introducing steam, and the digestion step is implemented until the desired degree of digestion has been reached. Also described is a cooking vessel that can be used when the method for digesting starch is being carried out.

With a method for digesting starch, an aqueous slurry of the starch is treated with steam in a cooking vessel and in this case exposed to shear forces, wherein the starch-containing slurry is heated to a temperature of between 85 C. and 110 C. in the cooking vessel by introducing steam, and the digestion step is implemented until the desired degree of digestion has been reached. Also described is a cooking vessel that can be used when the method for digesting starch is being carried out.