The invention relates to improved processes for the enzymatic production of allulose using enzymes which have been characterized as having improved expression, improved stability, and low allulose to fructose conversion activity, relative to enzymes in other allulose production methods. Improved processes include steps of converting fructose-6-phosphate to allulose 6-phopsphate A6P) using an allulose 6-phosphate epimerase, and converting A6P to allulose using an allulose-6-phosphate phosphatase.

The present invention relates to polypeptide, preferably to a glucose isomerase, comprising an amino acid sequence, wherein the amino acid sequence of the polypeptide, preferably the glucose isomerase, is at least 95% identical to an amino acid sequence of SEQ ID NO:1, wherein the amino acid sequence of the polypeptide, preferably the glucose isomerase, comprises an amino acid substitution at one or more amino acid positions, wherein the one or more amino acid positions is/are each and independently selected from the group consisting of SEQ ID NO: 1 amino acid positions 89, 90, 95, 0, 33, 34, 35, and 59. The present invention further relates to methods preparing glucose including the use of the polypeptides of the invention for preparing glucose.

The present invention relates to polypeptide, preferably to a glucose isomerase, comprising an amino acid sequence, wherein the amino acid sequence of the polypeptide, preferably the glucose isomerase, is at least 95% identical to an amino acid sequence of SEQ ID NO:1, wherein the amino acid sequence of the polypeptide, preferably the glucose isomerase, comprises an amino acid substitution at one or more amino acid positions, wherein the one or more amino acid positions is/are each and independently selected from the group consisting of SEQ ID NO: 1 amino acid positions 89, 90, 95, 0, 33, 34, 35, and 59. The present invention further relates to methods preparing glucose including the use of the polypeptides of the invention for preparing glucose.

The present invention relates to a composition for inhibiting liver fat accumulation comprising allulose as an active ingredient and a pharmaceutical composition and a food composition using thereof.

The present invention relates to a composition for inhibiting liver fat accumulation comprising allulose as an active ingredient and a pharmaceutical composition and a food composition using thereof.

The present application relates to a psicose-6-phosphate phosphatase, a microorganism comprising the same, and a method for producing psicose using the same.

The present invention relates to a composition for preparing tagatose, wherein the composition is used for preparing tagatose from fructose and contains a protein including any one amino acid sequence of SEQ ID NOS: 1 to 7 or a microorganism expressing the protein. In addition, the present invention relates to a method for preparing tagatose from fructose, the method comprising a step of allowing the composition to react with fructose.

The present invention relates to a composition for preparing tagatose, wherein the composition is used for preparing tagatose from fructose and contains a protein including any one amino acid sequence of SEQ ID NOS: 1 to 7 or a microorganism expressing the protein. In addition, the present invention relates to a method for preparing tagatose from fructose, the method comprising a step of allowing the composition to react with fructose.

A method of preparing D-psicose includes a step of reacting D-fructose as a substrate and an epimerase thereof in microorganisms at a temperature of 40° C. or higher. The method may further includes inducing the microorganisms to have resting cells by culturing the microorganisms in a medium not containing the D-fructose before the reaction. The method of preparing D-psicose may significantly improve the production amount of D-psicose and production rate of D-psicose.

A method of preparing D-psicose includes a step of reacting D-fructose as a substrate and an epimerase thereof in microorganisms at a temperature of 40° C. or higher. The method may further includes inducing the microorganisms to have resting cells by culturing the microorganisms in a medium not containing the D-fructose before the reaction. The method of preparing D-psicose may significantly improve the production amount of D-psicose and production rate of D-psicose.