The invention is directed to methods involved in the production of flavonoids, anthocyanins and other organic compounds. The invention provides cells engineered for the production of flavonoids, anthocyanins and other organic compounds, where the engineered cells include one or more genetic modifications that increase flavonoid production by increasing metabolic flux to flavonoid precursors and/or reducing carbon losses resulting from the production of byproducts.

The present invention relates to a microbial host cell genetically modified to intracellularly produce a cannabinoid glycoside, said cell expressing a heterologous gene encoding a glycosyl transferase which has a at least 70% identity to the glycosyl transferase comprised in SEQ ID NO: 157 or 207, capable of intracellularly glycosylating a cannabinoid acceptor with a glycosyl donor thereby producing the cannabinoid glycoside.

The present invention relates to a microbial host cell genetically modified to intracellularly produce a cannabinoid glycoside, said cell expressing a heterologous gene encoding a glycosyl transferase which has a at least 70% identity to the glycosyl transferase comprised in SEQ ID NO: 157 or 207, capable of intracellularly glycosylating a cannabinoid acceptor with a glycosyl donor thereby producing the cannabinoid glycoside.

Provided is a means for increasing mannosylerythritol lipid (MEL) production efficiency. The present invention is a mannosylerythritol-lipid-producing microorganism transformed with an expression vector having a gene that encodes a lipase under the control of E5Pgap promoter or E5Ptef promoter.

Provided is a means for increasing mannosylerythritol lipid (MEL) production efficiency. The present invention is a mannosylerythritol-lipid-producing microorganism transformed with an expression vector having a gene that encodes a lipase under the control of E5Pgap promoter or E5Ptef promoter.

The present disclosure relates to methods of producing organic compounds in a subterranean environment, and systems for the practice thereof. A benefit of the methods disclosed herein can include cost-effectively converting a carbon dioxide feedstock stored in a subterranean environment into one or more organic compounds.

The present invention provides an acidic sophorolipid-containing composition with suppressed browning and a method for producing the composition. The acidic sophorolipid-containing composition with suppressed browning according to the present invention is characterized in that a filtrate of a hydrolysate of a composition containing lactonic sophorolipid and acidic sophorolipid, i.e., a filtrate of the acidic sophorolipid-containing composition, has at least one of the following features (1) and (2): (1) hue value (Abs. 440): 5 or less; and (2) ethanol-insoluble content: less than 3 mass %.

In various aspects, the present invention is directed to a scalable method of producing rhamnolipids by bacterial fermentation with higher product concentrations, yields and productivities and preventing excessive foaming during the cell growth phase when the cell respiration rate is higher. It has been found that by slowing the growth rate of the bacteria by altering the ratio of the nitrogen source to the non-nitrogen source in the initial fermentation medium and supplementing the nitrogen source, excessive foaming in the growth phase can be prevented. Further, by using the non-nitrogen source as the limiting nutrient that initiates the stationary phase and then supplementing fermentation broth with the nitrogen and carbon sources, the length of the standing phase, and with it the time during which rhamnolipid production occurs can be greatly extended.

In various aspects, the present invention is directed to a scalable method of producing rhamnolipids by bacterial fermentation with higher product concentrations, yields and productivities and preventing excessive foaming during the cell growth phase when the cell respiration rate is higher. It has been found that by slowing the growth rate of the bacteria by altering the ratio of the nitrogen source to the non-nitrogen source in the initial fermentation medium and supplementing the nitrogen source, excessive foaming in the growth phase can be prevented. Further, by using the non-nitrogen source as the limiting nutrient that initiates the stationary phase and then supplementing fermentation broth with the nitrogen and carbon sources, the length of the standing phase, and with it the time during which rhamnolipid production occurs can be greatly extended.

Provided is a means for efficiently producing a monoacyl MEL. This means comprises culturing a monoacyl-MEL-producing microorganism in the presence of a surfactant.