The invention relates to a saccharose phosphorylase that catalyzes the synthesis of glucose-1-phosphate and fructose from saccharose and phosphate, among other things. The saccharose phosphorylase according to the invention can be considered to be a mutation of the saccharose phosphorylase from Bifidobacterium adolescentis. In comparison to wild-type saccharose phosphorylase, the saccharose phosphorylase according to the invention is distinguished by improved activity, process stability, temperature stability, and lower product inhibition and is therefore particularly suitable for use in industrial processes.

The invention relates to a saccharose phosphorylase that catalyzes the synthesis of glucose-1-phosphate and fructose from saccharose and phosphate, among other things. The saccharose phosphorylase according to the invention can be considered to be a mutation of the saccharose phosphorylase from Bifidobacterium adolescentis. In comparison to wild-type saccharose phosphorylase, the saccharose phosphorylase according to the invention is distinguished by improved activity, process stability, temperature stability, and lower product inhibition and is therefore particularly suitable for use in industrial processes.

The invention concerns a compound characterised by a mono(2-hydroxyethyl) terephthalic acid (MHET) and bis(2-hydroxyethyl) terephthalic acid chemically bonded to a saccharide. Furthermore, the invention concerns a corresponding compound which is used as a synthesis component for polymers or fine chemicals.

The invention concerns a compound characterised by a mono(2-hydroxyethyl) terephthalic acid (MHET) and bis(2-hydroxyethyl) terephthalic acid chemically bonded to a saccharide. Furthermore, the invention concerns a corresponding compound which is used as a synthesis component for polymers or fine chemicals.

Methods for removing heavy metals from contaminated water including contacting contaminated water with polysaccharides from N. meningitides serotypes B and W; a fusion gene product and fusion enzyme including silica acid synthase and CMP sialic acid synthetase, and use of the fusion enzyme in a simplified process to make CMP Sialic acid and derivatives thereof. Use of CMP Sialic acid and derivatives thereof to remove heavy metals from contaminated water.

Methods for removing heavy metals from contaminated water including contacting contaminated water with polysaccharides from N. meningitides serotypes B and W; a fusion gene product and fusion enzyme including silica acid synthase and CMP sialic acid synthetase, and use of the fusion enzyme in a simplified process to make CMP Sialic acid and derivatives thereof. Use of CMP Sialic acid and derivatives thereof to remove heavy metals from contaminated water.

2-6-Sialyltransferase (2,6ST) variants having improved 2-6-specific sialidase activity as compared to the native 2,6ST enzymes are described. The variants include GT80 sialyltransferases such as P. damselae Pd2,6ST. Methods for making de-sialylated products and screening sialidase activity are also described.

Process for producing a rhamnolipid produced by Pseudomonas or Enterobacter using andiroba or murumuru seed waste, pertaining to the sector of compounds containing monosaccharide radicals, consists of producing rhamnolipids by a biotechnological process using andiroba or murumuru seed waste, following oil extraction, as a substrate for a Pseudomonas aeruginosa, Enterobacter hormaechei or Enterobacter buriae line cultivated in a bioreactor with a non-dispersive aeration system for reducing foam, producing a rhamnolipid content of 10.5 g/L for Pseudomonas aeruginosa bacteria, in bioreactors carried out in a stirred tank with non-dispersive aeration using microporous membranes, particularly of silicone tubes, which allow oxygen to be supplied by diffusion. This type of aeration allows for various configurations, and in the embodiment of the invention, the porous membrane/tube was internally located in the liquid in the bioreactor in the form of a serpentine, under the following process conditions: pure oxygen with suitable pressure and flow rate to maintain O2 pressure in the bioreactor at 20% during the first 24 hours of the assay and stirring varying from 300 to 700 rpm, using 2 radial impellers and manual adjustment according to the decrease in the concentration of dissolved oxygen. The product produced has features that can be used primarily in the cosmetic industry due to its emulsifying, stability and non toxicity capacities.

Process for producing a rhamnolipid produced by Pseudomonas or Enterobacter using andiroba or murumuru seed waste, pertaining to the sector of compounds containing monosaccharide radicals, consists of producing rhamnolipids by a biotechnological process using andiroba or murumuru seed waste, following oil extraction, as a substrate for a Pseudomonas aeruginosa, Enterobacter hormaechei or Enterobacter buriae line cultivated in a bioreactor with a non-dispersive aeration system for reducing foam, producing a rhamnolipid content of 10.5 g/L for Pseudomonas aeruginosa bacteria, in bioreactors carried out in a stirred tank with non-dispersive aeration using microporous membranes, particularly of silicone tubes, which allow oxygen to be supplied by diffusion. This type of aeration allows for various configurations, and in the embodiment of the invention, the porous membrane/tube was internally located in the liquid in the bioreactor in the form of a serpentine, under the following process conditions: pure oxygen with suitable pressure and flow rate to maintain O2 pressure in the bioreactor at 20% during the first 24 hours of the assay and stirring varying from 300 to 700 rpm, using 2 radial impellers and manual adjustment according to the decrease in the concentration of dissolved oxygen. The product produced has features that can be used primarily in the cosmetic industry due to its emulsifying, stability and non toxicity capacities.

The invention relates to recombinant microorganisms and methods for producing steviol glycosides and steviol glycoside precursors.