A method of producing trilobatin is disclosed. The method comprising contacting a polypeptide comprising an amino acid sequence at least 90% identical to SEQ ID NO: 8 and having a 4-O-glycosyltransferase activity with phloretin and UDP-glucose under conditions which allow the formation of trilobatin, thereby producing trilobatin. A method of producing a plant which produces trilobatin, transgenic plant or plant cell and methods of producing transgenic plants are disclosed. Composition comprising trilobatin are also disclosed.

A method of producing trilobatin is disclosed. The method comprising contacting a polypeptide comprising an amino acid sequence at least 90% identical to SEQ ID NO: 8 and having a 4-O-glycosyltransferase activity with phloretin and UDP-glucose under conditions which allow the formation of trilobatin, thereby producing trilobatin. A method of producing a plant which produces trilobatin, transgenic plant or plant cell and methods of producing transgenic plants are disclosed. Composition comprising trilobatin are also disclosed.

Provided herein are methods and compositions for producing phenylpropanoid derivatives, such as chalcones and stilbenes, and dihydrophenylpropanoid derivatives, such as dihydrochalcones and dihydrostilbenes, in microorganisms. In particular, the disclosure provides recombinant microorganisms and methods of use thereof for producing phenylpropanoid derivative compounds and dihydrophenylpropanoid derivative compounds.

Provided herein are methods and compositions for producing phenylpropanoid derivatives, such as chalcones and stilbenes, and dihydrophenylpropanoid derivatives, such as dihydrochalcones and dihydrostilbenes, in microorganisms. In particular, the disclosure provides recombinant microorganisms and methods of use thereof for producing phenylpropanoid derivative compounds and dihydrophenylpropanoid derivative compounds.

The subject invention provides a novel SL that is widely applicable to various fields including food or beverages, cosmetics, pharmaceuticals, and quasi-drugs. A sophorolipid compound represented by Formula (I) below: ##STR00001##
(in Formula (I), R.sub.1 represents H or a methyl group; R.sub.3 and R.sub.4 each represents H or an acetyl group; one of five R.sub.5 is a saturated or unsaturated fatty acid residue that may have an OH group, and the remaining four R.sub.5 are H; R.sub.2 represents a C.sub.9-18 alkylene group, or C.sub.9-18 alkenylene group having 1 to 3 double bonds; R.sub.6 represents hydroxy, or may form a single bond together with one of five R.sub.7 in the compound represented by Formula (II) below) ##STR00002##
(in Formula (II), R.sub.1 represents H or methyl group; R.sub.3 and R.sub.4 each represents H or an acetyl group; R.sub.2 represents a C.sub.9-18 alkylene group, or C.sub.9-18 alkenylene group having 1 to 3 double bonds).

The subject invention provides a novel SL that is widely applicable to various fields including food or beverages, cosmetics, pharmaceuticals, and quasi-drugs. A sophorolipid compound represented by Formula (I) below: ##STR00001##
(in Formula (I), R.sub.1 represents H or a methyl group; R.sub.3 and R.sub.4 each represents H or an acetyl group; one of five R.sub.5 is a saturated or unsaturated fatty acid residue that may have an OH group, and the remaining four R.sub.5 are H; R.sub.2 represents a C.sub.9-18 alkylene group, or C.sub.9-18 alkenylene group having 1 to 3 double bonds; R.sub.6 represents hydroxy, or may form a single bond together with one of five R.sub.7 in the compound represented by Formula (II) below) ##STR00002##
(in Formula (II), R.sub.1 represents H or methyl group; R.sub.3 and R.sub.4 each represents H or an acetyl group; R.sub.2 represents a C.sub.9-18 alkylene group, or C.sub.9-18 alkenylene group having 1 to 3 double bonds).

The present invention relates to the production of steviol glycoside rebaudiosides D4, WB1 and WB2 and the production of rebaudioside M from Reb D4.

The purpose of the present invention is to: determine the structure of a novel steviol glycoside which is detected from species containing a large amount of Reb. C (also referred to as dulcoside B), and a trace amount of which is capable of influencing the quality of taste; and understand the taste characteristics of said steviol glycoside. The present invention provides a compound represented by formula (1) or a salt thereof, or a hydrate thereof.

##STR00001##

The purpose of the present invention is to: determine the structure of a novel steviol glycoside which is detected from species containing a large amount of Reb. C (also referred to as dulcoside B), and a trace amount of which is capable of influencing the quality of taste; and understand the taste characteristics of said steviol glycoside. The present invention provides a compound represented by formula (1) or a salt thereof, or a hydrate thereof.

##STR00001##

The present invention relates to genetically modified bacteria and methods of optimizing genetically modified bacteria for the production of a metabolite.