The purpose of the present invention is to: determine the structure of a novel steviol glycoside which is detected from species containing a large amount of Reb. C (also referred to as dulcoside B), and a trace amount of which is capable of influencing the quality of taste; and understand the taste characteristics of said steviol glycoside. The present invention provides a compound represented by formula (1) or a salt thereof, or a hydrate thereof. ##STR00001##

The purpose of the present invention is to: determine the structure of a novel steviol glycoside which is detected from species containing a large amount of Reb. C (also referred to as dulcoside B), and a trace amount of which is capable of influencing the quality of taste; and understand the taste characteristics of said steviol glycoside. The present invention provides a compound represented by formula (1) or a salt thereof, or a hydrate thereof. ##STR00001##

The disclosure discloses recombinant Bacillus subtilis for synthesizing e lacto-N-neotetraose yield. The recombinant Bacillus subtilis is obtained by integrating two -1,4-galactotransferase genes on a genome of a host bacterium Bacillus subtilis 168amyE:P.sub.43-lacY, P.sub.43-lgtB, P.sub.xylA-comK and exogenously expressing a -1,3-N-glucosaminotransferase gene. Compared with a strain before transformation, the recombinant Bacillus subtilis of the disclosure improves the yield of the synthesized lacto-N-neotetraose from 720 mg/L to 1300 mg/L, laying a foundation for further metabolic engineering transformation of Bacillus subtilis for producing the lacto-N-neotetraose.

The disclosure discloses recombinant Bacillus subtilis for synthesizing e lacto-N-neotetraose yield. The recombinant Bacillus subtilis is obtained by integrating two -1,4-galactotransferase genes on a genome of a host bacterium Bacillus subtilis 168amyE:P.sub.43-lacY, P.sub.43-lgtB, P.sub.xylA-comK and exogenously expressing a -1,3-N-glucosaminotransferase gene. Compared with a strain before transformation, the recombinant Bacillus subtilis of the disclosure improves the yield of the synthesized lacto-N-neotetraose from 720 mg/L to 1300 mg/L, laying a foundation for further metabolic engineering transformation of Bacillus subtilis for producing the lacto-N-neotetraose.

Provided are a Streptomyces avermitilis strain C63-51 with a high yield of ivermectin B1b and a method for producing ivermectin B1b by using this strain. By using the above-mentioned strain and method, efficient production of single ivermectin B1b can be achieved.

Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to express recombinant genes encoding UDP-glycosyltransferases (UGTs). Such microorganisms, plants, or plant cells can produce steviol glycosides, e.g., Rebaudioside A and/or Rebaudioside D, which can be used as natural sweeteners in food products and dietary supplements.

Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to express recombinant genes encoding UDP-glycosyltransferases (UGTs). Such microorganisms, plants, or plant cells can produce steviol glycosides, e.g., Rebaudioside A and/or Rebaudioside D, which can be used as natural sweeteners in food products and dietary supplements.

The present invention provides a method for preparing a mogroside having no -1,6-glucoside bond comprising the step of reacting glycosidase ASBGL2, AOBGL2, AOBGL1, ASBGL1, or a variant thereof with a mogroside having at least one -1,6-glucoside bond, thereby cleaving said -1,6-glucoside bond.

The present invention provides a method for preparing a mogroside having no -1,6-glucoside bond comprising the step of reacting glycosidase ASBGL2, AOBGL2, AOBGL1, ASBGL1, or a variant thereof with a mogroside having at least one -1,6-glucoside bond, thereby cleaving said -1,6-glucoside bond.

Process for producing a rhamnolipid produced by Pseudomonas or Enterobacter using andiroba or murumuru seed waste, pertaining to the sector of compounds containing monosaccharide radicals, consists of producing rhamnolipids by a biotechnological process using andiroba or murumuru seed waste, following oil extraction, as a substrate for a Pseudomonas aeruginosa, Enterobacter hormaechei or Enterobacter buriae line cultivated in a bioreactor with a non-dispersive aeration system for reducing foam, producing a rhamnolipid content of 10.5 g/L for Pseudomonas aeruginosa bacteria, in bioreactors carried out in a stirred tank with non-dispersive aeration using microporous membranes, particularly of silicone tubes, which allow oxygen to be supplied by diffusion. This type of aeration allows for various configurations, and in the embodiment of the invention, the porous membrane/tube was internally located in the liquid in the bioreactor in the form of a serpentine, under the following process conditions: pure oxygen with suitable pressure and flow rate to maintain O2 pressure in the bioreactor at 20% during the first 24 hours of the assay and stirring varying from 300 to 700 rpm, using 2 radial impellers and manual adjustment according to the decrease in the concentration of dissolved oxygen. The product produced has features that can be used primarily in the cosmetic industry due to its emulsifying, stability and non toxicity capacities.