The present invention relates to the production of steviol glycoside rebaudiosides D4, WB1 and WB2 and the production of rebaudioside M from Reb D4.

The invention relates to a method for production of tyrosol, wherein a transgenic bacterial cell that heterologously expresses phenylpyruvate decarboxylase and that overexpresses phospho-2-dehydro-3-deoxyheptonate and prephenate dehydrogenase, and wherein pheAL and feaB are both inactivated or removed, is grown in a medium comprising a metabolic precursor of phosphoenolpyruvate (PEP) and erythrose 4-phosphate (E4P), particularly glucose, and optionally, phenylalanine as a supplement; and tyrosol is extracted from said medium. The invention also relates to a method for production of salidroside, wherein the transgenic cell additionally heterologously expresses uridine diphosphate dependent glycosyltransferase (UGT85A1, EC:2.4.1.)

The invention relates to a method for production of tyrosol, wherein a transgenic bacterial cell that heterologously expresses phenylpyruvate decarboxylase and that overexpresses phospho-2-dehydro-3-deoxyheptonate and prephenate dehydrogenase, and wherein pheAL and feaB are both inactivated or removed, is grown in a medium comprising a metabolic precursor of phosphoenolpyruvate (PEP) and erythrose 4-phosphate (E4P), particularly glucose, and optionally, phenylalanine as a supplement; and tyrosol is extracted from said medium. The invention also relates to a method for production of salidroside, wherein the transgenic cell additionally heterologously expresses uridine diphosphate dependent glycosyltransferase (UGT85A1, EC:2.4.1.)

The present invention relates to an enzyme-catalyzed process for producing GDP-fucose from low-cost substrates guanosine and L-fucose or guanosine and D-Mannose in a single reaction mixture. Said process can be operated (semi)continuously or in batch mode. Further, said process can be adapted to produce fucosylated molecules and biomolecules including glycans, such as human milk oligosaccharides, proteins, peptides, glycoproteins or glycopeptides.

The present invention relates to an enzyme-catalyzed process for producing GDP-fucose from low-cost substrates guanosine and L-fucose or guanosine and D-Mannose in a single reaction mixture. Said process can be operated (semi)continuously or in batch mode. Further, said process can be adapted to produce fucosylated molecules and biomolecules including glycans, such as human milk oligosaccharides, proteins, peptides, glycoproteins or glycopeptides.

The invention relates to systems and methods for effective production and use of microorganisms and/or the fermentation broth in which they are produced. Advantageously, the system is cost-effective, scalable, quick, versatile, efficacious, and helpful in reducing resistance to chemical compounds and residue that concerns consumers.

The invention relates to systems and methods for effective production and use of microorganisms and/or the fermentation broth in which they are produced. Advantageously, the system is cost-effective, scalable, quick, versatile, efficacious, and helpful in reducing resistance to chemical compounds and residue that concerns consumers.

The subject invention provides environmentally-friendly compositions and methods for enhancing production of textiles and leather. In certain embodiments, the methods of the subject invention comprise incorporating the application of a biological amphiphilic molecule into a textile or leather-making process to reduce chemical usage, reduce water usage, reduce water pollution and/or provide an added benefit to the process. In certain embodiments, the methods of the subject invention comprise substituting a chemical surfactant with a biological amphiphilic molecule in one or more steps involved in textile or leather-making process that would traditionally utilize a chemical surfactant.

The subject invention provides environmentally-friendly compositions and methods for enhancing production of textiles and leather. In certain embodiments, the methods of the subject invention comprise incorporating the application of a biological amphiphilic molecule into a textile or leather-making process to reduce chemical usage, reduce water usage, reduce water pollution and/or provide an added benefit to the process. In certain embodiments, the methods of the subject invention comprise substituting a chemical surfactant with a biological amphiphilic molecule in one or more steps involved in textile or leather-making process that would traditionally utilize a chemical surfactant.

The subject invention provides systems and apparatuses for producing microbe-based compositions that can be used in the oil and gas industry, environmental cleanup, as well as for other applications. More specifically, the present invention includes biological reactors, equipment, and materials for fermenting microbe-based compositions.