Provided is a method for improving the yield of rhamnolipids comprising culturing in medium containing a triglyceride containing oil and sweetener as a carbon source.

Provided is a method for improving the yield of rhamnolipids comprising culturing in medium containing a triglyceride containing oil and sweetener as a carbon source.

Pseudomonas chlororaphis NRRL B-30761 produces monorhamnolipids with predominantly 3-hydroxydodecenoyl-3-hydroxydecanoate (C.sub.12:1-C.sub.10) or 3-hydroxydodecanoyl-3-hydroxydecanoate (C.sub.12-C.sub.10) as the lipid moiety under static growth conditions. The cloning and sequencing of three genes and proteins involved in the biosynthesis of monorhamnose-lipid (R.sub.1L) is described. Expression of two of these genes, i.e., rhlA and rhlB, together in P. chlororaphis NRRL B-30761 increases R.sub.1L production by at least 10-fold. Also the generation of a recombinant P. chlororaphis NRRL B-30761 capable of synthesizing dirhamnose-lipid (R.sub.2L) is described. Characterization of R.sub.1L and R.sub.2L produced by the recombinant P. chlororaphis NRRL B-30761 is also described.

Pseudomonas chlororaphis NRRL B-30761 produces monorhamnolipids with predominantly 3-hydroxydodecenoyl-3-hydroxydecanoate (C.sub.12:1-C.sub.10) or 3-hydroxydodecanoyl-3-hydroxydecanoate (C.sub.12-C.sub.10) as the lipid moiety under static growth conditions. The cloning and sequencing of three genes and proteins involved in the biosynthesis of monorhamnose-lipid (R.sub.1L) is described. Expression of two of these genes, i.e., rhlA and rhlB, together in P. chlororaphis NRRL B-30761 increases R.sub.1L production by at least 10-fold. Also the generation of a recombinant P. chlororaphis NRRL B-30761 capable of synthesizing dirhamnose-lipid (R.sub.2L) is described. Characterization of R.sub.1L and R.sub.2L produced by the recombinant P. chlororaphis NRRL B-30761 is also described.

The present invention relates to a method of preparing at least one rhamnolipid comprising: contacting a recombinant cell with a medium containing a carbon source; and culturing the cell under suitable conditions for preparation of the rhamnolipid from the carbon source by the cell, wherein the recombinant cell has been genetically modified such that, compared to the wild-type of the cell, the cell has an increased activity of at least one of the enzymes E.sub.1, E.sub.2 and E.sub.3, wherein the enzyme E.sub.1 is an / hydrolase, the enzyme E.sub.2 is a rhamnosyltransferase I and the enzyme E.sub.3 is a rhamnosyl-transferase II, and wherein the carbon source is a C.sub.4 molecule.

The present invention relates to a method of preparing at least one rhamnolipid comprising: contacting a recombinant cell with a medium containing a carbon source; and culturing the cell under suitable conditions for preparation of the rhamnolipid from the carbon source by the cell, wherein the recombinant cell has been genetically modified such that, compared to the wild-type of the cell, the cell has an increased activity of at least one of the enzymes E.sub.1, E.sub.2 and E.sub.3, wherein the enzyme E.sub.1 is an / hydrolase, the enzyme E.sub.2 is a rhamnosyltransferase I and the enzyme E.sub.3 is a rhamnosyl-transferase II, and wherein the carbon source is a C.sub.4 molecule.

1-2-fucosyltransferases, and methods and compositions for making and using 1-2-fucosyltransferases, are described herein.

1-2-fucosyltransferases, and methods and compositions for making and using 1-2-fucosyltransferases, are described herein.

The present invention relates to a composition comprising one or more steviol glycosides which composition comprises nitrogen in an amount of no more than about 1000 ppm. The invention also relates to a method for preparing a steviol glycoside composition, which method comprises: providing a steviol glycoside composition; combining the steviol glycoside composition with water to form a steviol glycoside solution; and crystallizing a steviol glycoside composition from the solution. The invention also relates to a method for reducing the nitrogen content of a steviol glycoside composition, which method comprises: providing a steviol glycoside composition which comprises nitrogen; combining the steviol glycoside composition with water to form a steviol glycoside solution; and crystallizing a steviol glycoside composition from the solution, thereby to reduce the amount of nitrogen in the steviol glycoside composition.

Methods for producing anthocyanin by expression in a microorganism are disclosed including culturing of the microorganism under anthocyanin producing conditions, wherein the microorganism has an operative metabolic pathway including at least one heterologous enzyme activity, the pathway producing anthocyanin from simple sugars or other simple carbon sources.