The methods and compositions described herein relate to the identification, isolation, and characterization of genes which encode proteins useful for the biosynthesis of transglycosylase inhibitors such as moes. The methods and compositions also relate to the production of such proteins, and their use in the synthesis of moes, the expression of moes, and the production of modified moes.

The methods and compositions described herein relate to the identification, isolation, and characterization of genes which encode proteins useful for the biosynthesis of transglycosylase inhibitors such as moes. The methods and compositions also relate to the production of such proteins, and their use in the synthesis of moes, the expression of moes, and the production of modified moes.

Provided is a means for increasing mannosylerythritol lipid (MEL) production efficiency. The present invention is a mannosylerythritol-lipid-producing microorganism transformed with an expression vector having a gene that encodes a lipase under the control of E5Pgap promoter or E5Ptef promoter.

Provided is a means for increasing mannosylerythritol lipid (MEL) production efficiency. The present invention is a mannosylerythritol-lipid-producing microorganism transformed with an expression vector having a gene that encodes a lipase under the control of E5Pgap promoter or E5Ptef promoter.

The present invention relates to a recombinant microbial system for generation of neo-glycocins, a substrate and to co-evolve its glycosyltransferase enzyme. The recombinant microbial system comprises a gene cassette A encoding for microbial O- and S-glycosyltransferase and its suitable acceptor substrate in conjugation with a cleavable dual affinity tag under the control of two independent inducible promoters. The gene cassette is expressed in a microbial host such as E. coli for the co-expression of glycosyltransferase and its suitable acceptor substrate. The invention further discloses method for production and bioactivity guided screening of O- and or S-neo-glycocins using the recombinant microbial system. The system provides optimized construct design, and methods for high yield production of glycocins and neo-glycocins for downstream applications.

The present invention relates to a recombinant microbial system for generation of neo-glycocins, a substrate and to co-evolve its glycosyltransferase enzyme. The recombinant microbial system comprises a gene cassette A encoding for microbial O- and S-glycosyltransferase and its suitable acceptor substrate in conjugation with a cleavable dual affinity tag under the control of two independent inducible promoters. The gene cassette is expressed in a microbial host such as E. coli for the co-expression of glycosyltransferase and its suitable acceptor substrate. The invention further discloses method for production and bioactivity guided screening of O- and or S-neo-glycocins using the recombinant microbial system. The system provides optimized construct design, and methods for high yield production of glycocins and neo-glycocins for downstream applications.

Biomass (e.g., plant biomass, animal biomass, microbial, and municipal waste biomass) is processed to produce useful products, such as food products and amino acids.

Biomass (e.g., plant biomass, animal biomass, microbial, and municipal waste biomass) is processed to produce useful products, such as food products and amino acids.

The subject invention provides microbe-based products, as well as their use to improve oil production and refining efficiency by, for example, remediating the disposable layers in oil tanks and other oil storage units. In preferred embodiments, the microbe-based products comprise biochemical-producing yeast and growth by-products thereof, such as, e.g., biosurfactants. The subject invention can be used to remediate rag layer and/or other dissolved solid layers that form in water-oil emulsions. Furthermore, the subject invention can be used for remediating solid impurities, such as sand, scale, rust and clay, in produced water, flow-back, brine, and/or fracking fluids.

The subject invention provides microbe-based products, as well as their use to improve oil production and refining efficiency by, for example, remediating the disposable layers in oil tanks and other oil storage units. In preferred embodiments, the microbe-based products comprise biochemical-producing yeast and growth by-products thereof, such as, e.g., biosurfactants. The subject invention can be used to remediate rag layer and/or other dissolved solid layers that form in water-oil emulsions. Furthermore, the subject invention can be used for remediating solid impurities, such as sand, scale, rust and clay, in produced water, flow-back, brine, and/or fracking fluids.