The invention relates to systems and methods for effective production and use of microorganisms and/or the fermentation broth in which they are produced. Advantageously, the system is cost-effective, scalable, quick, versatile, efficacious, and helpful in reducing resistance to chemical compounds and residue that concerns consumers.

Methods for preparing at least one glycolipid of formula (I): [Glc]n-xOy-R (I), with [Glc]n representing an osidic motif comprising 1-7 n glucosyl units; R representing a fatty acid radical with 4-24 carbon atoms; -xOy- symbolizing the attachment of the fatty acid radical to the osidic motif; the method comprising glucosylating a hydroxylated fatty acid of formula (II): R-yOH (II), where -yOH is a hydroxyl group attached to a carbon atom Cy; bringing the hydroxylated fatty acid of formula (II) into contact with at least one -transglucosylase of the GH70 family in the presence of saccharose or a saccharose analogue.

Methods for preparing at least one glycolipid of formula (I): [Glc]n-xOy-R (I), with [Glc]n representing an osidic motif comprising 1-7 n glucosyl units; R representing a fatty acid radical with 4-24 carbon atoms; -xOy- symbolizing the attachment of the fatty acid radical to the osidic motif; the method comprising glucosylating a hydroxylated fatty acid of formula (II): R-yOH (II), where -yOH is a hydroxyl group attached to a carbon atom Cy; bringing the hydroxylated fatty acid of formula (II) into contact with at least one -transglucosylase of the GH70 family in the presence of saccharose or a saccharose analogue.

Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to express recombinant genes encoding UDP-glycosyltransferases (UGTs). Such microorganisms, plants, or plant cells can produce steviol glycosides, e.g., Rebaudioside A and/or Rebaudioside D, which can be used as natural sweeteners in food products and dietary supplements.

Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to express recombinant genes encoding UDP-glycosyltransferases (UGTs). Such microorganisms, plants, or plant cells can produce steviol glycosides, e.g., Rebaudioside A and/or Rebaudioside D, which can be used as natural sweeteners in food products and dietary supplements.

The subject invention provides materials and methods for producing high-HLB sophorolipid-based compositions and/or for increasing the water solubility of sophorolipid compositions. More specifically, the subject invention provides for the production and post-fermentation alteration of SLP molecules, which can be used for formulating a variety of useful compositions.

The subject invention provides materials and methods for producing high-HLB sophorolipid-based compositions and/or for increasing the water solubility of sophorolipid compositions. More specifically, the subject invention provides for the production and post-fermentation alteration of SLP molecules, which can be used for formulating a variety of useful compositions.

A method of making glycomonolipids; said method comprising the following steps: a) reacting glycodilipids with a hydrolase to form glycomonolipids; and b) optionally isolating the glycomonolipids.

Glucosylated chlorogenic acid (GCGA) compounds, particularly compounds selected from those having structural formulae (A), (B), (C), (D), (E), (F), salts thereof, and combinations thereof, and methods of preparing same are described. The GCGA compounds are useful in beverage products, food products, and home care products.

The invention provides materials and method for controlling pests, in particular, nematodes. The invention also provides compositions comprising biosurfactants as pesticides.