The disclosure provides genetically engineered microorganisms capable of producing antigen-binding molecules. Additionally, the disclosure provides engineered microorganisms comprising one or more disrupted genes to strategically divert carbon flux away from undesirable products towards products, and optionally co-products, of interest. Further, the disclosure enables co-production of useful chemicals from gaseous substrates.

Described herein are compositions of hydrogels and methods of use thereof.

Described herein are compositions of hydrogels and methods of use thereof.

A method and use of nisin-permeabilized microbial cells as whole-cell catalysts for reducing the amount of a target substrate in a sample to one of more product are provided. Specifically, a method of reducing the amount of lactose in a dairy sample using nisin-permeabilized lactic acid bacterial cell catalysts, which have been permeabilized by incubating with a nisin producing microbial cell and/or culture medium derived therof. Further provided is a nisin producing microbial cell, derived from parent strain Lactococcus lactis subsp. lactis bv. diacetylactis SD96 (NCBI accession No. SRX6686433).

A method and use of nisin-permeabilized microbial cells as whole-cell catalysts for reducing the amount of a target substrate in a sample to one of more product are provided. Specifically, a method of reducing the amount of lactose in a dairy sample using nisin-permeabilized lactic acid bacterial cell catalysts, which have been permeabilized by incubating with a nisin producing microbial cell and/or culture medium derived therof. Further provided is a nisin producing microbial cell, derived from parent strain Lactococcus lactis subsp. lactis bv. diacetylactis SD96 (NCBI accession No. SRX6686433).

The disclosure provides biosynthetic platforms that generate olivetolic acid and its analogues at high titers from microbes, and in cell free systems.

A hangover relieving composition including a dry powder, lysate or extract of yeast that produces glutathione and acetaldehyde dehydrogenase. Further, embodiments relate to a hangover relieving composition containing the dry powder, lysate or extract of Saccharomyces cerevisiae Kwon P-1 KCTC13925BP and Saccharomyces cerevisiae Kwon P-2 KCTC14122BP and Saccharomyces cerevisiae Kwon P-3 KCTC14123BP yeast that simultaneously produce glutathione and acetaldehyde dehydrogenase.

A hangover relieving composition including a dry powder, lysate or extract of yeast that produces glutathione and acetaldehyde dehydrogenase. Further, embodiments relate to a hangover relieving composition containing the dry powder, lysate or extract of Saccharomyces cerevisiae Kwon P-1 KCTC13925BP and Saccharomyces cerevisiae Kwon P-2 KCTC14122BP and Saccharomyces cerevisiae Kwon P-3 KCTC14123BP yeast that simultaneously produce glutathione and acetaldehyde dehydrogenase.

A method for manufacturing a fermentation product by culturing a microorganism, the method containing steps (A) to (D): (A) culturing the microorganism with a first culture medium; (B) passing a culture solution containing cultured bacterial cells, raw materials for culture, and the fermentation product through an adsorption tower packed with an adsorbent capable of adsorbing the fermentation product from the culture solution, and then collecting an effluent containing the bacterial cells and the raw materials for culture flowing out from the adsorption tower, wherein a relationship among a size (short diameter) d of the bacterial cell, a pore size D1 of the adsorbent, and a minimum void size D2 between adsorbent particles, D1<d<D2 is satisfied; (C) eluting the fermentation product from the absorbent; and (D) culturing the microorganism with a second culture medium using collected effluent containing the bacterial cells and the raw materials for culture.

A method for manufacturing a fermentation product by culturing a microorganism, the method containing steps (A) to (D): (A) culturing the microorganism with a first culture medium; (B) passing a culture solution containing cultured bacterial cells, raw materials for culture, and the fermentation product through an adsorption tower packed with an adsorbent capable of adsorbing the fermentation product from the culture solution, and then collecting an effluent containing the bacterial cells and the raw materials for culture flowing out from the adsorption tower, wherein a relationship among a size (short diameter) d of the bacterial cell, a pore size D1 of the adsorbent, and a minimum void size D2 between adsorbent particles, D1<d<D2 is satisfied; (C) eluting the fermentation product from the absorbent; and (D) culturing the microorganism with a second culture medium using collected effluent containing the bacterial cells and the raw materials for culture.