Systems and methods for rapid determination of microorganism growth and antimicrobial agent susceptibility and/or resistance are disclosed.

Systems and methods for rapid determination of microorganism growth and antimicrobial agent susceptibility and/or resistance are disclosed.

The current disclosure relates to therapeutic treatments and diagnostic methods for esophageal cancer based on the expression level of biomarker miRNAs. Aspects of the disclosure relate to a method of treating esophageal cancer (EC) in a patient, said method comprising: diagnosing the patient with esophageal cancer when the patient is determined to have an elevated or decreased level of expression of one or more miRNAs selected from mir-15b, miR-17, mir-18a, mir-21, mir-23a, mir-24-2, mir-25, mir-27a, mir-93, mir-103, mir-106b, mir-129-2, mir-139, mir-146b, mir-148a, mir-151, miR-155, mir-181a-1, mir-181a, mir-181b-1, mir-181b, mir-182, mir-183, mir-192, mir-194-1, mir-194-2, mir-196a-1, mir-196a-2, mir-196b, mir-205, mir-215, mir-223, mir-224, mir-335, mir-338, mir-375, mir-421, mir-484, mir-505, mir-769, mir-944, mir-1468, mir-3648, and let-7i in a sample from a patient relative to the expression level of the one or more miRNAs in a control sample; and administering an effective amount of an esophageal treatment to the diagnosed patient.

The invention relates to the field of biotechnology and pharmaceutics. Proposed is a method for ultra-high performance screening of biological objects which is based on microfluidic generation of droplets of a biocompatible water-in-oil-in-water double emulsion, and also a method for producing a monodisperse biocompatible water-in-oil-in-water double emulsion. The invention can be used in diagnosing conditions and diseases in mammals, as well as for investigating biological objects.

The invention relates to the field of biotechnology and pharmaceutics. Proposed is a method for ultra-high performance screening of biological objects which is based on microfluidic generation of droplets of a biocompatible water-in-oil-in-water double emulsion, and also a method for producing a monodisperse biocompatible water-in-oil-in-water double emulsion. The invention can be used in diagnosing conditions and diseases in mammals, as well as for investigating biological objects.

The present invention provides liquid crystal (LC)-infused materials and methods for detecting compounds or impurities in liquid samples using such materials. These slippery materials comprise a lubricating liquid, preferably a thermotropic liquid crystal, and a solid substrate able to immobilize or host the lubricating liquid. The portion of the substrate coated by the lubricating fluid forms a slippery surface able to allow droplets of various materials to slide off the slippery surface in a manner dependent on the chemical composition of the droplet, which can be used to detect the presence of analytes, impurities and other molecules within the droplet.

The present invention provides liquid crystal (LC)-infused materials and methods for detecting compounds or impurities in liquid samples using such materials. These slippery materials comprise a lubricating liquid, preferably a thermotropic liquid crystal, and a solid substrate able to immobilize or host the lubricating liquid. The portion of the substrate coated by the lubricating fluid forms a slippery surface able to allow droplets of various materials to slide off the slippery surface in a manner dependent on the chemical composition of the droplet, which can be used to detect the presence of analytes, impurities and other molecules within the droplet.

Provided is a cell analysis device including: a display unit; a storage unit; an analysis result input receiving unit to receive inputs of culture conditions and measured values of items obtained by analyzing a cell cultured under each condition, and store them in the storage unit associating the measured values with a corresponding one culture condition; a culture condition designation input receiving unit to receive input of designation of the culture condition; a correlation evaluation unit to read out measured values of items on the cell cultured under the designated culture condition from the storage unit, and evaluate a magnitude of correlation between the measured values of two items for all combinations of two of the items; and a correlation item presentation unit to display, on the display unit, a predetermined number of combinations extracted from among all the combinations based on an evaluation result by the correlation evaluation unit.

An object of the present invention is to provide a method of producing an intestinal epithelial cell, which has a large number of cells per area and a high accuracy of kinetic prediction for a CYP3A4 substrate drug such as midazolam, by inducing the differentiation of a pluripotent stem cell, as well as the intestinal epithelial cell, a cell sheet, an evaluation method for a test substance, a screening kit for a test substance, and a cell preparation. According to the present invention, there is provided a production method for an intestinal epithelial cell, including a first differentiation step of differentiating a pluripotent stem cell into an intestinal stem cell, a proliferation step of proliferating the intestinal stem cell obtained in the differentiation step, and a second differentiation step of differentiating the intestinal stem cell obtained in the proliferation step into an intestinal epithelial cell, in which the proliferation step is a step of bringing the intestinal stem cell into a specific state.

With the aim of proving an antibacterial composition against oral bacteria and the like capable of inducing Th1 cell proliferation or activation in an intestinal tract, the present inventors have found out that bacteria that suppress colonization and the like of the oral bacteria and the like in the intestinal tract are present in an intestinal microbiota. Moreover, the present inventors have succeeded in isolating intestinal bacteria that suppress intestinal colonization and the like of oral bacteria and the like.