A microfabricated device having at least one gas-entrapping feature formed therein in a configuration that entraps air bubbles upon wetting the feature with a solvent or solution is described. The device includes a sacrificial residue in contact with the gas-entrapping feature, the dissolution of which guides the wetting of the gas-entrapping feature.

A microfabricated device having at least one gas-entrapping feature formed therein in a configuration that entraps air bubbles upon wetting the feature with a solvent or solution is described. The device includes a sacrificial residue in contact with the gas-entrapping feature, the dissolution of which guides the wetting of the gas-entrapping feature.

The present invention is concerned with a method for detection of presence and/or quantities of silver nanoparticles in a specimen. The method includes the steps of a) providing a detection organism suspended in a medium, b) treating the detection organism with zinc ions thus effecting auto-fluorescence therefrom, and then measuring degree of fluorescence of the detection organism suspended medium, c) adding the specimen to the detection organism suspended medium, treating the detection organism therein with the specimen for a period of time, and measuring change of fluorescence of the detection organism-suspended medium over time, d) calculating amount of silver ions intracellularly dissolved from the silver nanoparticles and accumulated in the detection organism in view of the change of fluorescence, and e) extrapolating quantity of silver nanoparticles in the specimen in view of the change of fluorescence and the amount of the intracellularly dissolved silver ions.

The present invention is concerned with a method for detection of presence and/or quantities of silver nanoparticles in a specimen. The method includes the steps of a) providing a detection organism suspended in a medium, b) treating the detection organism with zinc ions thus effecting auto-fluorescence therefrom, and then measuring degree of fluorescence of the detection organism suspended medium, c) adding the specimen to the detection organism suspended medium, treating the detection organism therein with the specimen for a period of time, and measuring change of fluorescence of the detection organism-suspended medium over time, d) calculating amount of silver ions intracellularly dissolved from the silver nanoparticles and accumulated in the detection organism in view of the change of fluorescence, and e) extrapolating quantity of silver nanoparticles in the specimen in view of the change of fluorescence and the amount of the intracellularly dissolved silver ions.

Detecting single bacterial cells in a sample includes collecting, from a sample provided to an imaging apparatus, a multiplicity of images of the sample over a length of time; assessing a trajectory of each bacterial cell in the sample; and assessing, based on the trajectory of each bacterial cell in the sample, a number of bacterial cell divisions that occur in the sample during the length of time.

Provided herein are compounds useful for imaging granzyme B. An exemplary compound provided herein is useful as a radiotracer for position emission tomography (PET) and/or single photon emission tomography (SPECT) imaging. Methods of imaging granzyme B, combination therapies, and kits comprising the granzyme B imaging agents are also provided.

Provided herein are compounds useful for imaging granzyme B. An exemplary compound provided herein is useful as a radiotracer for position emission tomography (PET) and/or single photon emission tomography (SPECT) imaging. Methods of imaging granzyme B, combination therapies, and kits comprising the granzyme B imaging agents are also provided.

The present invention is intended to provide a cell treatment apparatus and a method for treating cells that can suppress a variation of a treatment time of treating cells using laser light. The cell treatment apparatus of the present invention includes: a cell treatment chamber in which cells in a cell culture vessel are treated; an observation unit that can observe the cells; a laser projection unit that can project a laser image onto the cells; a laser moving unit that can move the laser projection unit; and a control unit. The laser projection unit includes: a laser light source; and a laser image generation portion that generates the laser image to be projected onto the cells from laser light oscillated from the laser light source. The control unit controls generation of the laser image by the laser image generation portion. By moving the laser moving unit from a projection start position of the laser image at one end of the cell culture vessel to a projection end position of the laser image at the other end of the cell culture vessel, the laser projection unit projects the laser image from the projection start position of the laser image at one end of the cell culture vessel to the projection end position of the laser image at the other end of the cell culture vessel.

Disclosed herein include embodiments of a system, a device, and a method for sorting a plurality cells of a sample. A plurality of raw images comprising pixels of complex values in a frequency space can be generated from a plurality of channels of fluorescence intensity data of fluorescence emissions of fluorophores, the fluorescence emissions being elicited by fluorescence imaging using radiofrequency-multiplexed excitation in a temporal space. Spectral unmixing can be performed on the raw images prior to a sorting decision being made.

Disclosed herein include embodiments of a system, a device, and a method for sorting a plurality cells of a sample. A plurality of raw images comprising pixels of complex values in a frequency space can be generated from a plurality of channels of fluorescence intensity data of fluorescence emissions of fluorophores, the fluorescence emissions being elicited by fluorescence imaging using radiofrequency-multiplexed excitation in a temporal space. Spectral unmixing can be performed on the raw images prior to a sorting decision being made.