Described are coelenterazine analogues, methods for making the analogues, kits comprising the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays.

The present disclosure provides a method and a platform for enhancing detection activity of an interaction between a spike protein receptor binding domain of coronavirus from a specimen and a human angiotensin-converting enzyme II. The method and the platform of the present disclosure use a cleavable luciferase as a report test for the combination of the spike protein receptor binding domain of coronavirus (such as novel coronavirus) and angiotensin-converting enzyme II. Screening is carried out at the cellular level. The strength of the drug's influence on the interaction between the two molecules can be judged by the strength of the luminescence signal. The detection time can be completed within 20 minutes.

Provided herein are compositions and methods for stabilizing coelenterazine and analogs or derivatives thereof, and for improving the solubility and reconstitution efficiency of coelenterazine and analogs and derivatives thereof.

The present invention provides a method for assessing the possibility of onset or progression of chronic kidney allograft rejection or chronic kidney disease in a test subject, by using, as an indicator, the level of SYT17 protein in a urine-derived specimen collected from the test subject. The present invention also provides: a pharmaceutical composition for preventing and/or treating chronic kidney allograft rejection or chronic kidney disease, the pharmaceutical composition including at least one substance selected from the group consisting of a specific antibody to SYT17, a derivative of the specific antibody, and an inhibitory nucleic acid against SYT17; and a test kit for use in assessing the possibility of onset or progression of chronic kidney allograft rejection or chronic kidney disease, the test kit including a specific antibody to SYT17 or a derivative of the specific antibody.

The present invention provides a cell-based assay for measuring T cell activation mediated by a T cell-dependent bispecific antibody (TDB). In some aspects, the assay is useful for detecting a TDB in a composition, quantitating the amount of TDB in a composition, determining the potency and/or specificity of a TDB, or determining if a population of cells expresses a target antigen. Compositions and kits are also contemplated.

The present invention provides a cell-based assay for measuring T cell activation mediated by a T cell-dependent bispecific antibody (TDB). In some aspects, the assay is useful for detecting a TDB in a composition, quantitating the amount of TDB in a composition, determining the potency and/or specificity of a TDB, or determining if a population of cells expresses a target antigen. Compositions and kits are also contemplated.

It is an object of the present invention to provide a fluorescence imaging probe capable of selectively visualizing target cells such as cells expressing β-galactosidase (lacZ expressing cells) at a single-cell level in a red fluorescence region, and of performing co-staining together with GFP. An intracellularly-retainable red fluorescent probe comprising a compound represented by the following formula (I) or a salt thereof: ##STR00001## wherein: A represents a monovalent group cleaved by an enzyme; R.sup.1 represents a hydrogen atom, or one to four of the same or different substituents bonded to a benzene ring; R.sup.3, R.sup.4, R.sup.5, and R.sup.6 each independently represent —CFR.sup.10R.sup.11, —CF.sub.2R.sup.12, a hydrogen atom, a hydroxyl group, an alkyl group, or a halogen atom, wherein at least one of R.sup.3, R.sup.4, R.sup.5, and R.sup.6 is —CFR.sup.10R.sup.11 or —CF.sub.2R.sup.12; R.sup.2 and R.sup.7 each independently represent a hydrogen atom, a hydroxyl group, an alkyl group, or a halogen atom; R.sup.8 and R.sup.9 each independently represent a hydrogen atom or an alkyl group; R.sup.10, R.sup.11, and R.sup.12 each independently represent a hydrogen atom, an alkyl group, or an alkenyl group; X represents Si(R.sup.a) (R.sup.b), wherein R.sup.a and R.sup.b each independently represent a hydrogen atom or an alkyl group; and Y is —C(═O)— or —R.sup.cC(═O)—, wherein R.sup.c is an alkylene group having 1-3 carbon atoms.

It is an object of the present invention to provide a fluorescence imaging probe capable of selectively visualizing target cells such as cells expressing β-galactosidase (lacZ expressing cells) at a single-cell level in a red fluorescence region, and of performing co-staining together with GFP. An intracellularly-retainable red fluorescent probe comprising a compound represented by the following formula (I) or a salt thereof: ##STR00001## wherein: A represents a monovalent group cleaved by an enzyme; R.sup.1 represents a hydrogen atom, or one to four of the same or different substituents bonded to a benzene ring; R.sup.3, R.sup.4, R.sup.5, and R.sup.6 each independently represent —CFR.sup.10R.sup.11, —CF.sub.2R.sup.12, a hydrogen atom, a hydroxyl group, an alkyl group, or a halogen atom, wherein at least one of R.sup.3, R.sup.4, R.sup.5, and R.sup.6 is —CFR.sup.10R.sup.11 or —CF.sub.2R.sup.12; R.sup.2 and R.sup.7 each independently represent a hydrogen atom, a hydroxyl group, an alkyl group, or a halogen atom; R.sup.8 and R.sup.9 each independently represent a hydrogen atom or an alkyl group; R.sup.10, R.sup.11, and R.sup.12 each independently represent a hydrogen atom, an alkyl group, or an alkenyl group; X represents Si(R.sup.a) (R.sup.b), wherein R.sup.a and R.sup.b each independently represent a hydrogen atom or an alkyl group; and Y is —C(═O)— or —R.sup.cC(═O)—, wherein R.sup.c is an alkylene group having 1-3 carbon atoms.

A method for detecting the presence of bacterial spores is described by measuring microbial metabolic activity over time. Spores are distinguished from vegetative cells and other microorganisms by detecting a burst of metabolic activity indicating germination of spores. This method may be used to detect bacterial spores in a commercial process system, such as a papermaking system within the time frame of a typical work shift.

A method for detecting the presence of bacterial spores is described by measuring microbial metabolic activity over time. Spores are distinguished from vegetative cells and other microorganisms by detecting a burst of metabolic activity indicating germination of spores. This method may be used to detect bacterial spores in a commercial process system, such as a papermaking system within the time frame of a typical work shift.