An ultraviolet (UV) absorbance assay for measuring the concentration of large RNA molecules such as mRNA in suspensions comprising RNA-lipid nanoparticles (RNA-LNPs) is described.

Provided herein are methods of detecting one or more nucleic acids in a biofluid sample. The methods include adding to the biofluid sample a composition comprising a sufficient amount of dextran sulphate to provide between 50 nM and 5 μM dextran sulphate when the composition is added to the biofluid sample.

Provided herein are methods of detecting one or more nucleic acids in a biofluid sample. The methods include adding to the biofluid sample a composition comprising a sufficient amount of dextran sulphate to provide between 50 nM and 5 μM dextran sulphate when the composition is added to the biofluid sample.

The present application relates to compositions and methods for sequencing by synthesis, where one or more palladium scavengers were used to improve sequencing metrics such phasing and prephasing values.

The present application relates to compositions and methods for sequencing by synthesis, where one or more palladium scavengers were used to improve sequencing metrics such phasing and prephasing values.

The present invention relates to the field of viral nucleic acid detection. In particular, the present invention provides a pretreatment method for viral nucleic acid detection. The method includes mixing a pretreatment solution containing a sample with a nucleic acid releasing agent and a qPCR amplification reagent, wherein the pretreatment solution includes Tris-HCl, EDTA-2Na, sodium chloride, a ribonuclease (RNase) inhibitor, and an antibiotic; and the pretreatment solution has a pH of 6.5-8.0.

The present invention relates to the field of viral nucleic acid detection. In particular, the present invention provides a pretreatment method for viral nucleic acid detection. The method includes mixing a pretreatment solution containing a sample with a nucleic acid releasing agent and a qPCR amplification reagent, wherein the pretreatment solution includes Tris-HCl, EDTA-2Na, sodium chloride, a ribonuclease (RNase) inhibitor, and an antibiotic; and the pretreatment solution has a pH of 6.5-8.0.

A method of biochip production includes a step A of applying a solution containing a selective binding substance, a salt, and a condensing agent to the surface of a substrate, a step B of immobilizing the selective binding substance to the surface of the substrate, a step C of drying the applied solution to allow the salt in the solution to precipitate on the surface of the substrate, and a step D of using an image detection device to detect a precipitate of the salt formed in the step C, wherein, in the step A, the condensing agent in the solution has a concentration of not less than 30 mM and not more than 80 mM.

A method of biochip production includes a step A of applying a solution containing a selective binding substance, a salt, and a condensing agent to the surface of a substrate, a step B of immobilizing the selective binding substance to the surface of the substrate, a step C of drying the applied solution to allow the salt in the solution to precipitate on the surface of the substrate, and a step D of using an image detection device to detect a precipitate of the salt formed in the step C, wherein, in the step A, the condensing agent in the solution has a concentration of not less than 30 mM and not more than 80 mM.

The present invention provides a method for detecting Brucella infection, i.e., a serum and blood cell synchronous detection method. The detection method comprises two operation steps of serum sample detection and living blood cell sample detection and uses a supporting kit. The kit can be used for pretreatment of blood samples for clinically detecting Brucella in vitro. The serum and blood cell synchronous detection method can be used for early clinical rapid diagnosis of Brucella infection and medication guidance in the treatment process, and can also be used for prognosis, epidemiological survey of brucellosis, etc. The present invention can also be used for early clinical rapid diagnosis of other intracellular parasitic infection.