The present disclosure provides systems, devices, and methods for purifying microorganism nucleic acid from a host sample, such as a whole blood sample from a human. In certain embodiments, devices and systems with multiple filters are employed and provide for the selective removal of blood cells and host nucleic acids from a sample in order to enrich for microorganism nucleic acid.

The present disclosure provides systems, devices, and methods for purifying microorganism nucleic acid from a host sample, such as a whole blood sample from a human. In certain embodiments, devices and systems with multiple filters are employed and provide for the selective removal of blood cells and host nucleic acids from a sample in order to enrich for microorganism nucleic acid.

The present disclosure provides methods for the isolation of a cell free complex comprising a circulating, cell free nucleic acid component. The nucleic acid component of the complex is, at least in part, associated with cellular components, such as polypeptides and lipids. The methods of the present disclosure provide for the isolation of circulating cell free nucleic acids that are longer and less fragmented as compared to prior art methods. Furthermore, the circulating, cell free nucleic acid represent biologically relevant nucleic acid targets as the methods described selectively remove non-functional nucleic acids from the isolated material. The nucleic acid component of the isolated complexes reflects a wide spectrum of genomic representation enabling successful detection of mutations, polymorphisms, methylation status and other genomic markers for use in diagnostic and therapeutic applications. Furthermore, the additional cellular components in the cell free complexes isolated provide information regarding the proteomic and lipidomic status of the subject

The present disclosure provides methods for the isolation of a cell free complex comprising a circulating, cell free nucleic acid component. The nucleic acid component of the complex is, at least in part, associated with cellular components, such as polypeptides and lipids. The methods of the present disclosure provide for the isolation of circulating cell free nucleic acids that are longer and less fragmented as compared to prior art methods. Furthermore, the circulating, cell free nucleic acid represent biologically relevant nucleic acid targets as the methods described selectively remove non-functional nucleic acids from the isolated material. The nucleic acid component of the isolated complexes reflects a wide spectrum of genomic representation enabling successful detection of mutations, polymorphisms, methylation status and other genomic markers for use in diagnostic and therapeutic applications. Furthermore, the additional cellular components in the cell free complexes isolated provide information regarding the proteomic and lipidomic status of the subject

The disclosure provides methods of characterizing a sample of oligonucleotides of interest using liquid chromatography and mass spectrometry.

The disclosure provides methods of characterizing a sample of oligonucleotides of interest using liquid chromatography and mass spectrometry.

The present invention relates to a magnetic gradient-based nucleic acid isolation kit that provides nucleic acid (RNA/DNA) isolation from biological materials. The kit comprises a flat-bottomed tube (1) made of glass or its derivatives, configured to contain biological material: a modular and reusable magnetic gradient patch (2) that is made of ferromagnetic metal powder and polymer, that is positioned outside and below the tube such that it is not in direct contact with the biological material to be placed in the tube (1), that is in direct contact with the entire flat bottom of the tube (1); and a mold (3) positioned under the flat-bottom tube (1), configured such that the magnetic gradient patch (1) is put into it. By means of the present invention, a kit is provided for target nucleic acid retention that does not require the direct binding of magnetic elements to nucleic acids and their cleaning, that has a magnetic gradient patch that can be used repeatedly, that accelerates and facilitates the nucleic acid isolation step, thereby accelerating the process in studies that require the use of nucleic acids.

The present invention relates to a magnetic gradient-based nucleic acid isolation kit that provides nucleic acid (RNA/DNA) isolation from biological materials. The kit comprises a flat-bottomed tube (1) made of glass or its derivatives, configured to contain biological material: a modular and reusable magnetic gradient patch (2) that is made of ferromagnetic metal powder and polymer, that is positioned outside and below the tube such that it is not in direct contact with the biological material to be placed in the tube (1), that is in direct contact with the entire flat bottom of the tube (1); and a mold (3) positioned under the flat-bottom tube (1), configured such that the magnetic gradient patch (1) is put into it. By means of the present invention, a kit is provided for target nucleic acid retention that does not require the direct binding of magnetic elements to nucleic acids and their cleaning, that has a magnetic gradient patch that can be used repeatedly, that accelerates and facilitates the nucleic acid isolation step, thereby accelerating the process in studies that require the use of nucleic acids.

Techniques for replacing nanopores within a nanopore based sequencing chip are provided. A first electrolyte solution is added to the external reservoir of the sequencing chip, introducing an osmotic imbalance between the reservoir and the well chamber located on the opposite side of a lipid bilayer membrane. The osmotic imbalance causes the membrane to change shape, and a nanopore within the membrane to be ejected. A second electrolyte solution is then added to the external reservoir to provide replacement nanopores and to restore the membrane shape. The replacement nanopores can be inserted into the membrane, effectively replacing the initial pore without causing the destruction of the membrane.

Techniques for replacing nanopores within a nanopore based sequencing chip are provided. A first electrolyte solution is added to the external reservoir of the sequencing chip, introducing an osmotic imbalance between the reservoir and the well chamber located on the opposite side of a lipid bilayer membrane. The osmotic imbalance causes the membrane to change shape, and a nanopore within the membrane to be ejected. A second electrolyte solution is then added to the external reservoir to provide replacement nanopores and to restore the membrane shape. The replacement nanopores can be inserted into the membrane, effectively replacing the initial pore without causing the destruction of the membrane.