Provided among other things is a method of applying force to a macromolecule such as dsDNA to make an end more accessible, such as more accessible to ligation.

Provided among other things is a method of applying force to a macromolecule such as dsDNA to make an end more accessible, such as more accessible to ligation.

Provided in the present invention are a splint nucleic acid molecule for cyclizing a single-stranded nucleic acid molecule and an application therefor. The splint nucleic acid. molecule is composed of a 5 terminal fragment and a 3 terminal fragment, the 5 terminal fragment being adapted to forming a first complementary region with a 5 terminal of the single-stranded nucleic acid molecule, and the 3 terminal fragment being suited to forming a second complementary region with a 3 terminal of the single-stranded nucleic acid molecule, the length of the first complementary region and the second complementary region being different.

Provided in the present invention are a splint nucleic acid molecule for cyclizing a single-stranded nucleic acid molecule and an application therefor. The splint nucleic acid. molecule is composed of a 5 terminal fragment and a 3 terminal fragment, the 5 terminal fragment being adapted to forming a first complementary region with a 5 terminal of the single-stranded nucleic acid molecule, and the 3 terminal fragment being suited to forming a second complementary region with a 3 terminal of the single-stranded nucleic acid molecule, the length of the first complementary region and the second complementary region being different.

The present invention relates to a kit for detecting miRNA and a method for detecting miRNA using the kit. According to the miRNA detection kit and method of the present invention, it is possible to detect a certain miRNA in a quick and accurate manner, and it also possible to perform multiplex analysis capable of detecting a plurality of miRNAs at the same time.

The present invention relates to a kit for detecting miRNA and a method for detecting miRNA using the kit. According to the miRNA detection kit and method of the present invention, it is possible to detect a certain miRNA in a quick and accurate manner, and it also possible to perform multiplex analysis capable of detecting a plurality of miRNAs at the same time.

Provided are methods and systems for encoding data into nucleic acid molecules. Methods and systems disclosed can include the use of promiscuous template nucleic acid molecules which enables data encoding using environmental modifications to yield encoded nucleic acid molecules.

A primer set includes a terminal primer A including, in its 3-terminal part, a nucleotide sequence that hybridizes to a 3-terminal part of a complementary sequence of a first target nucleic acid sequence, a k-th double-headed primer including two polynucleotides linked at their 5 terminal sides, wherein one of the two polynucleotides includes, in its 3-terminal part, a nucleotide sequence that hybridizes to a 3-terminal part of a nucleotide sequence of a k-th target nucleic acid, and the other polynucleotide includes, in its 3-terminal part, a nucleotide sequence that hybridizes to a 3-terminal part of a complementary sequence of a (k+1)th target nucleic acid, and a terminal primer B including, in its 3-terminal part, a nucleotide sequence that hybridizes to a 3-terminal part of a nucleotide sequence of a N-th target nucleic acid.

A primer set includes a terminal primer A including, in its 3-terminal part, a nucleotide sequence that hybridizes to a 3-terminal part of a complementary sequence of a first target nucleic acid sequence, a k-th double-headed primer including two polynucleotides linked at their 5 terminal sides, wherein one of the two polynucleotides includes, in its 3-terminal part, a nucleotide sequence that hybridizes to a 3-terminal part of a nucleotide sequence of a k-th target nucleic acid, and the other polynucleotide includes, in its 3-terminal part, a nucleotide sequence that hybridizes to a 3-terminal part of a complementary sequence of a (k+1)th target nucleic acid, and a terminal primer B including, in its 3-terminal part, a nucleotide sequence that hybridizes to a 3-terminal part of a nucleotide sequence of a N-th target nucleic acid.

Methods and kits for joining fragmented nucleic acid sequences together are provided, including performing an amplifying step including contacting a sample suspected of including a fragmented target nucleic acid with a pair of external primers and a pair of self-complementary internal primers, and generating a full length target nucleic acid. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, kits are provided that are designed for the detection of a target nucleic acid sequence.