Methods for the rapid detection of the presence or absence of Herpes simplex virus 1 and 2 (HSV-1 and HSV-2) in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers, probes targeting the genes for HSV-1 viral DNA polymerase B (HSV-1 Pol) and HSV-1 thymidine kinase C (HSV-1 TK), and also target the genes for HSV-2 thymidine kinase C (HSV-2 TK) and HSV-2 glycoprotein B (HSV-2 gB), along with kits are provided that are designed for the detection of HSV-1 and/or HSV-2.

Methods for the rapid detection of the presence or absence of Herpes simplex virus 1 and 2 (HSV-1 and HSV-2) in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers, probes targeting the genes for HSV-1 viral DNA polymerase B (HSV-1 Pol) and HSV-1 thymidine kinase C (HSV-1 TK), and also target the genes for HSV-2 thymidine kinase C (HSV-2 TK) and HSV-2 glycoprotein B (HSV-2 gB), along with kits are provided that are designed for the detection of HSV-1 and/or HSV-2.

Methods and kits for determining the efficiency of plasma separation from whole blood are provided, using real-time PCR amplification of two amplicons, namely, a short amplicon of e.g. 70-150 bps and a long amplicon of e.g. 350-600 bps. The separation efficiency is determined based on the difference in amplification patterns of the two amplicons.

Methods and kits for determining the efficiency of plasma separation from whole blood are provided, using real-time PCR amplification of two amplicons, namely, a short amplicon of e.g. 70-150 bps and a long amplicon of e.g. 350-600 bps. The separation efficiency is determined based on the difference in amplification patterns of the two amplicons.

The invention relates to methods, kits and uses thereof for detecting gene mutations. The method comprises the step of: providing a mixture of nucleic acid molecules comprising both a genomic DNA and a RNA molecule derived from said genomic DNA, performing a reverse transcription reaction with a reverse transcriptase to provide cDNA molecules from said RNA molecule in said mixture; and performing PCR using both said genomic DNA and said cDNA molecules as templates. This method can simultaneously detect a trace amount of a mutation in both DNA and RNA from a whole blood sample using a small amount of sample. At the same time, by adjusting the reaction system, the detection limit of a mutant in a background of wild-type molecules can reach 0.01% or lower.

The invention relates to methods, kits and uses thereof for detecting gene mutations. The method comprises the step of: providing a mixture of nucleic acid molecules comprising both a genomic DNA and a RNA molecule derived from said genomic DNA, performing a reverse transcription reaction with a reverse transcriptase to provide cDNA molecules from said RNA molecule in said mixture; and performing PCR using both said genomic DNA and said cDNA molecules as templates. This method can simultaneously detect a trace amount of a mutation in both DNA and RNA from a whole blood sample using a small amount of sample. At the same time, by adjusting the reaction system, the detection limit of a mutant in a background of wild-type molecules can reach 0.01% or lower.

The present invention discloses primers, probes, a kit and a method for detecting Enterocytozoon hepatopenaei (EHP) pathogens of Litopenaeus vannamei. A PCR reaction system adopted in the method includes: a forward primer having a nucleotide sequence as shown in SEQ ID NO: 1, a reverse primer having a nucleotide sequence as shown in SEQ ID NO: 2, a forward primer having a nucleotide sequence as shown in SEQ ID NO: 4, a reverse primer having a nucleotide sequence as shown in SEQ ID NO: 5, a probe having a nucleotide sequence as shown in SEQ ID NO: 3, and a probe having a nucleotide sequence as shown in SEQ ID NO: 6. The method can perform duplex quantitative fluorescence PCR, an internal reference and a sample to be detected are put in the same reaction, thereby eliminating unstable factors of two reactions and improving the reliability of the result.

The present invention discloses primers, probes, a kit and a method for detecting Enterocytozoon hepatopenaei (EHP) pathogens of Litopenaeus vannamei. A PCR reaction system adopted in the method includes: a forward primer having a nucleotide sequence as shown in SEQ ID NO: 1, a reverse primer having a nucleotide sequence as shown in SEQ ID NO: 2, a forward primer having a nucleotide sequence as shown in SEQ ID NO: 4, a reverse primer having a nucleotide sequence as shown in SEQ ID NO: 5, a probe having a nucleotide sequence as shown in SEQ ID NO: 3, and a probe having a nucleotide sequence as shown in SEQ ID NO: 6. The method can perform duplex quantitative fluorescence PCR, an internal reference and a sample to be detected are put in the same reaction, thereby eliminating unstable factors of two reactions and improving the reliability of the result.

Methods and compositions for the detection and quantification of nucleic acids are provided. In certain embodiments, methods involve the use of cleavable probes that comprise a ribonucleotide position that is susceptible to endoribonuclease (e.g., RNase H) cleavage in the presence of target nucleic acid molecules. Probes of the embodiments may also comprise non-natural nucleotide linked to a reporter and/or quenching moiety.

Methods and compositions for the detection and quantification of nucleic acids are provided. In certain embodiments, methods involve the use of cleavable probes that comprise a ribonucleotide position that is susceptible to endoribonuclease (e.g., RNase H) cleavage in the presence of target nucleic acid molecules. Probes of the embodiments may also comprise non-natural nucleotide linked to a reporter and/or quenching moiety.