Provided is a labeling method for nucleic acid including a reaction step for hybridizing a nucleic acid probe that has a nucleotide sequence complementary to that of a nucleic acid to be labeled and contains a reactive nucleobase derivative incorporated at a position complementary to that of a target nucleobase as a target of labeling in the nucleic acid to be labeled, to the nucleic acid to be labeled; a transferring step for transferring a transfer group contained in the reactive nucleobase derivative to the nucleotide residue containing the target nucleobase in the nucleic acid to be labeled; and a labeling step for labeling the transfer group transferred to the nucleotide residue with a radioactive material.

Provided is a labeling method for nucleic acid including a reaction step for hybridizing a nucleic acid probe that has a nucleotide sequence complementary to that of a nucleic acid to be labeled and contains a reactive nucleobase derivative incorporated at a position complementary to that of a target nucleobase as a target of labeling in the nucleic acid to be labeled, to the nucleic acid to be labeled; a transferring step for transferring a transfer group contained in the reactive nucleobase derivative to the nucleotide residue containing the target nucleobase in the nucleic acid to be labeled; and a labeling step for labeling the transfer group transferred to the nucleotide residue with a radioactive material.

A method is described to define the binding of fragments onto RNA targets and to use this profiling to enable the design of small molecules targeting RNA. The method comprises exposing a labeled RNA target to a small molecule fragment appended with diazirine and an alkyne moiety. Exposure of the compounds to light produce a reactive intermediate from the diazirine moiety that will react with sites in the RNA that are proximal to the small molecule fragments binding site. The RNAs that are reacted with the fragments are captured by using a biotin azide or azide-displaying beads that react with the alkyne moiety in the presence of a Cu(I) catalyst using click chemistry. Biotinylated products are captured with streptavidin resin. The amount of labeled RNA captured by the resin/beads is measured, thereby identifying which fragments bind an RNA target. The binding site of the fragment is determined by RT-PCR.

A method is described to define the binding of fragments onto RNA targets and to use this profiling to enable the design of small molecules targeting RNA. The method comprises exposing a labeled RNA target to a small molecule fragment appended with diazirine and an alkyne moiety. Exposure of the compounds to light produce a reactive intermediate from the diazirine moiety that will react with sites in the RNA that are proximal to the small molecule fragments binding site. The RNAs that are reacted with the fragments are captured by using a biotin azide or azide-displaying beads that react with the alkyne moiety in the presence of a Cu(I) catalyst using click chemistry. Biotinylated products are captured with streptavidin resin. The amount of labeled RNA captured by the resin/beads is measured, thereby identifying which fragments bind an RNA target. The binding site of the fragment is determined by RT-PCR.

Nucleotides are provided with at least one isotope. The isotope-modified nucleotides can be used for data storage, increasing the data density compared to only natural nucleotides. Described is a method of storing data on a DNA strand, the method comprising providing a DNA strand having at least one isotope-modified nucleotide comprising at least one isotope of carbon, nitrogen, oxygen or hydrogen, assigning a bit pattern to the at least one isotope-modified nucleotide that is different than a bit pattern assigned to a non-isotope-modified nucleotide. Data could be stored on any molecule that can be isotope-modified.

Nucleotides are provided with at least one isotope. The isotope-modified nucleotides can be used for data storage, increasing the data density compared to only natural nucleotides. Described is a method of storing data on a DNA strand, the method comprising providing a DNA strand having at least one isotope-modified nucleotide comprising at least one isotope of carbon, nitrogen, oxygen or hydrogen, assigning a bit pattern to the at least one isotope-modified nucleotide that is different than a bit pattern assigned to a non-isotope-modified nucleotide. Data could be stored on any molecule that can be isotope-modified.

Methods of sequencing and producing nucleic acid sequences are provided. Accordingly there are provided methods of sequencing a nucleic acid sequence comprising L-nucleotides comprising subjecting the nucleic acid sequence comprising the L-nucleotides to a chemical sequencing method. Also provided is a method of reverse transcribing a ribose nucleic acid sequence into a deoxyribose nucleic acid sequence comprising catalyzing reverse transcription of the ribose nucleic acid sequence with a Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4).

Methods of sequencing and producing nucleic acid sequences are provided. Accordingly there are provided methods of sequencing a nucleic acid sequence comprising L-nucleotides comprising subjecting the nucleic acid sequence comprising the L-nucleotides to a chemical sequencing method. Also provided is a method of reverse transcribing a ribose nucleic acid sequence into a deoxyribose nucleic acid sequence comprising catalyzing reverse transcription of the ribose nucleic acid sequence with a Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4).

In order to quantitatively analyze nucleic acids present in a sample or a complex medium, a nucleic acid extraction or purification process is required. However, the yield of nucleic acid extraction and purification is greatly variable depending on the purification principle and the characteristics of kit and sample used. Hence, efficient normalization of nucleic acid extraction and purification yield is a prerequisite for accurate quantitative analysis of nucleic acid based on the original sample. The present invention relates to a quantitative analysis method of a nucleic acid present in a sample or a complex medium without amplification of a target nucleic acid.

In order to quantitatively analyze nucleic acids present in a sample or a complex medium, a nucleic acid extraction or purification process is required. However, the yield of nucleic acid extraction and purification is greatly variable depending on the purification principle and the characteristics of kit and sample used. Hence, efficient normalization of nucleic acid extraction and purification yield is a prerequisite for accurate quantitative analysis of nucleic acid based on the original sample. The present invention relates to a quantitative analysis method of a nucleic acid present in a sample or a complex medium without amplification of a target nucleic acid.