The present disclosure discloses a real-time fluorescence quantitative polymerase chain reaction (PCR) detection method and kit based on a metal ruthenium complex. The present disclosure is capable of establishing the detection method for performing a real-time fluorescence quantitative PCR by using the metal ruthenium complex as fluorescence dye.

The present disclosure discloses a real-time fluorescence quantitative polymerase chain reaction (PCR) detection method and kit based on a metal ruthenium complex. The present disclosure is capable of establishing the detection method for performing a real-time fluorescence quantitative PCR by using the metal ruthenium complex as fluorescence dye.

Provided herein are compositions, systems, and methods for detecting microorganisms. In particular, provided herein are compositions, systems, and methods for rapid, multiplex detection of microorganism in unpurified biological samples.

Provided herein are compositions, systems, and methods for detecting microorganisms. In particular, provided herein are compositions, systems, and methods for rapid, multiplex detection of microorganism in unpurified biological samples.

Method and composition for identifying cognate nucleotides in a Sequencing By Binding™ procedure, wherein one or more labeled nucleotides are detected in ternary complexes but never incorporated. Labeled nucleotides can be incorporable nucleotides that contact preformed blocked primed template nucleic acids. Alternatively, labeled nucleotides are labeled non-incorporable nucleotides. Labeled nucleotides, including labeled non-incorporable nucleotides, can be detected in ternary complexes in the same reaction mixture that incorporates a reversible terminator nucleotide to create a blocked primed template nucleic acid. Detection of ternary complexes can take place in the presence of a catalytic metal ion.

Method and composition for identifying cognate nucleotides in a Sequencing By Binding™ procedure, wherein one or more labeled nucleotides are detected in ternary complexes but never incorporated. Labeled nucleotides can be incorporable nucleotides that contact preformed blocked primed template nucleic acids. Alternatively, labeled nucleotides are labeled non-incorporable nucleotides. Labeled nucleotides, including labeled non-incorporable nucleotides, can be detected in ternary complexes in the same reaction mixture that incorporates a reversible terminator nucleotide to create a blocked primed template nucleic acid. Detection of ternary complexes can take place in the presence of a catalytic metal ion.

The invention displays aflatoxin B.sub.1 (AFB.sub.1) detection kit and AFB.sub.1 detection method. The invention belongs to the technical field of detecting harmful substances. The AFB.sub.1 detection kit was fabricated with DNA walker structure, endonuclease, hairpin H1 and H2. The AFB.sub.1 detection kit has benefits of high sensitivity and short detection time based on signal amplification strategy of DNA Walker structure and hyperbranched fluorescent nanotrees. The present invention can realize high sensitive and rapid detection of AFB.sub.1.

The invention displays aflatoxin B.sub.1 (AFB.sub.1) detection kit and AFB.sub.1 detection method. The invention belongs to the technical field of detecting harmful substances. The AFB.sub.1 detection kit was fabricated with DNA walker structure, endonuclease, hairpin H1 and H2. The AFB.sub.1 detection kit has benefits of high sensitivity and short detection time based on signal amplification strategy of DNA Walker structure and hyperbranched fluorescent nanotrees. The present invention can realize high sensitive and rapid detection of AFB.sub.1.

The present disclosure provides a low cost, high sensitivity, high specificity and rapid diagnostic apparatus and method to detect nucleic acids in a sample at room temperature. As low as tens of copies of nucleic acids can be detected without any additional equipment.

The present disclosure provides a low cost, high sensitivity, high specificity and rapid diagnostic apparatus and method to detect nucleic acids in a sample at room temperature. As low as tens of copies of nucleic acids can be detected without any additional equipment.