Methods and compositions for attaching cell-specific barcodes without formation of partitions is provided.

The present disclosure provides methods and systems for sequencing nucleic acid molecules using a single frequency during detection, or fewer frequencies than types of nucleotide bases identified during detection. Methods and systems of the present disclosure may involve transiently binding nucleotides. Methods and systems provided herein may enable sequences to be determined at a higher accuracy and efficiency.

The present disclosure provides methods and systems for sequencing nucleic acid molecules using a single frequency during detection, or fewer frequencies than types of nucleotide bases identified during detection. Methods and systems of the present disclosure may involve transiently binding nucleotides. Methods and systems provided herein may enable sequences to be determined at a higher accuracy and efficiency.

Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.

Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.

The present disclosure provides methods and systems for detecting nucleic acid sequences in a biological sample having a three-dimensional matrix. The present disclosure also provides methods and systems for processing a biological sample for use in nucleic acid sequence detection.

The present disclosure provides methods and systems for detecting nucleic acid sequences in a biological sample having a three-dimensional matrix. The present disclosure also provides methods and systems for processing a biological sample for use in nucleic acid sequence detection.

A method for extracting a target nucleic acid from a sample liquid includes providing a heating device having a heating element in contact with the sample liquid. The heating element is conjugated with at least one functional nucleic acid. The functional nucleic acid is adapted to hybridize to the target nucleic acid and bind the target nucleic acid to the heating element. Further, the method includes generating relative movement between the heating element and the sample liquid and extracting the target nucleic acid from the sample liquid by separating the heating element from the sample liquid.

A method for extracting a target nucleic acid from a sample liquid includes providing a heating device having a heating element in contact with the sample liquid. The heating element is conjugated with at least one functional nucleic acid. The functional nucleic acid is adapted to hybridize to the target nucleic acid and bind the target nucleic acid to the heating element. Further, the method includes generating relative movement between the heating element and the sample liquid and extracting the target nucleic acid from the sample liquid by separating the heating element from the sample liquid.

A method for identifying a predefined target organism includes extracting a nucleic acid from a sample to form an extracted nucleic acid, amplifying the extracted nucleic acid to form a nucleic acid amplicon, tagging the nucleic acid amplicon with a capture probe and a detector partner to form a detector partner-nucleic acid amplicon-capture probe complex, and performing a detection assay on the detector partner-nucleic acid amplicon-capture probe complex to identify whether the predefined target organism is present in the sample.