A biomolecular characteristic identification method includes: breeding a plurality of Taiwan Tilapias in a predetermined environment to obtain a plurality of baited Taiwan Tilapias; seeking at least one nucleotide mark in the baited Taiwan Tilapias to obtain at least one feature sequence marker therefrom; producing at least one design of primer pair according to the feature sequence marker for identification; and identifying an unknown DNA sample of the baited Taiwan Tilapias with the at least one primer pair in a biomolecular tracing procedure to obtain an identification result.

The present disclosure provides a molecular marker for identifying traits of efficiency of duck feed utilization based on a neuropeptide gene NPY, a method and use thereof. The NPY gene has a nucleotide sequence shown in SEQ ID NO: 1, the molecular marker is T or C, and the molecular marker is located at position 577 of the nucleotide sequence. In the present disclosure, mutation of the NPY gene is detected using a polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) method, the traits of efficiency of duck feed utilization are selected based on genotypes, and a breeding method for early selection of efficiency of poultry feed utilization is established. The method is simple, rapid, and low-cost, does not need special instrument, and satisfies experimental needs.

The present disclosure relates methods and compositions useful for prevention of porcine reproductive and respiratory syndrome virus (PRRSv) in animals, including animals of the species Sus scrofa. The present teachings relate to swine wherein at least one allele of a CD163 gene has been inactivated, and to specific methods and nucleic acid sequences used in gene editing to inactivate the CD163 gene. Swine wherein both alleles of the CD163 gene are inactivated are resistant to porcine reproductive and respiratory syndrome virus (PRRSv). Elite lines comprising homozygous CD163 edited genes retain their superior properties.

A process for analysing chromosome regions and interactions relating to physical performance.

Described herein are assays and kits capable of specifically detecting Enterocytozoon hepatopenaei (EHP) in a nucleic acid sample. In some embodiments, the nucleic acid sample is obtained from shrimp tissue, shrimp feed, or a feed ingredient.

Disclosed are nucleic acid molecules from the genome of Dirofilaria spp. nematodes that contain single nucleotide polymorphisms related to reduced responsiveness of the nematodes to macrocyclic lactones. In one example, the species of Dirofilaria is Dirofilaria immitis (the agent of heartworm in animals). Also disclosed are methods for determining the responsiveness of Dirofilaria spp. nematodes to macrocyclic lactones, methods for selecting a treatment to treat an animal infected with a Dirofilaria spp. nematode, and kits for determining the responsiveness of Dirofilaria spp. nematodes to macrocyclic lactones.

The invention features methods, systems, and panels for rapid detection of tick-borne pathogens in a sample (including Borrelia spp. such as B. burgdorferi, B. afzelii, and B. garinii) and for diagnosis and monitoring of tick-transmitted diseases, including Lyme disease, Rocky Mountain spotted fever, Q-fever, babesiosis, ehrlichiosis, tularemia, and anaplasmosis.

The invention encompasses methods for increasing genetic progress in livestock, and for genetic dissemination, including the use of amniocentesis to obtain fetal amniocytes for use in genomic evaluation and cloning.

The invention provides methods of predicting the weight loss propensity of an animal of the genus Equus, through the assessment of various markers in a sample obtained from the animal. Such markers include fermentation products and/or metabolites thereof such as volatile fatty acids, indicators of bacterial population diversity, and the abundance/relative abundance of specific bacterial taxa.

The present disclosure provides an in vitro method of generating bovine monocyte-derived macrophages from monocytes that produce nitric oxide and use as an indicator of innate immune response potential. The culture system includes culturing bovine monocytes in serum-free media supplemented with granulocyte-macrophage stimulating factor (GM-CSF) to generate monocyte-derived macrophages that produce nitric oxide.